Report of Mollicutes in the Ear Canal of Domestic Dogs in Brazil - Open Access Library Journal

OALibJ > Vol.3 No.7, July 2016

Report of Mollicutes in the Ear Canal of Domestic Dogs in Brazil ()

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DOI: 10.4236/oalib.1102790 836 Downloads 1,454 Views Citations

Author(s)

Sandra Batista dos Santos^*, Maína de Souza Almeida, Luana Thamires Rapôso da Silva, Júnior Mário Baltazar de Oliveira, Carlos Adriano de Santana Leal, José Wilton Pinheiro Júnior, Rinaldo Aparecido Mota

Affiliation(s)

Laboratory of Infectious Disease of Animals, Universidade Federal Rural de Pernambuco, Recife, Brazil.

ABSTRACT

Several microorganisms as bacteria, fungi, yeast and ectoparasites compose canine ear canal microbiota. These agents can be native or invasive pathogens that cause clinical otitis. The aim of this study was to associate Mollicutes in external ear canal with clinical otitis. 41 domestic dogs were examined, and a total of 82 otologic samples were collected using sterile swabs, placed in a plastic storage tube containing 2 milliliters of sterile phosphate buffer saline (PBS, pH 7.2) and appropriately labeled. The samples were processed for isolation of Mollicutes using Hayflick’s medium. The frequency of Mollicutes class in the isolation was 32.9% (27/82). 14.8% (4/27) of the dogs, which were positive for Mollicutes, had external otitis and 85.2% (23/27) were healthy dogs. The genus Mycoplasma spp. was detected in 7.4% (2/27) by digitonin test. It was the first isolation of Mollicutes from the external ear canal in dogs. Although there have been important findings in this study, the presence of these bacteria cannot indicate the onset and perpetuation of external otitis in dogs.

KEYWORDS

Mollicutes, Otites, Canines, Microbiota, Brazil

Share and Cite:

Santos, S. , Almeida, M. , Silva, L. , Oliveira, J. , Leal, C. , Júnior, J. and Mota, R. (2016) Report of Mollicutes in the Ear Canal of Domestic Dogs in Brazil. Open Access Library Journal, 3, 1-5. doi: 10.4236/oalib.1102790.

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