Author(s)

Yan Ma^*, Yan Lin, Liping Sun, Lina Song, Rongfeng Cui, Yibin Qie

Beijing Key Laboratory of Green Plants Breeding, Beijing Institute of Landscape Architecture, Beijing, China.

Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII), with five various fluence ranges (1 × 10⁹ - 1 × 10¹¹ ions/cm²) of 40 MeV and four various fluence ranges (1 × 10¹⁰ - 5 × 10¹¹ ions/cm²) of 35 MeV, respectively. M₁ generations of various SII-treated Oenothera speciosa lines can overwinter, and the highest overwinter rate (41.3%) was observed in Oenothera speciosa lines treated with 35 MeV and fluence 5 × 10¹⁰ ions/cm². M₂ and M₃ generations of all treated lines were able to overwinter smoothly. The results indicated that SII treatment can enhance the cold-resistance of Oenothera speciosa heritably. Furthermore, physiological indexes including relative electrical conductivity, MDA contents and proline contents of SII-treated Oenothera speciosa pot seedlings were detected after low temperature stress. The results revealed that relative electrical conductivities and MDA contents of M₁, M₂ and M₃ generations of SII-treated Oenothera speciosa plants were lower than that of control, whereas the proline contents were higher than control in the -5^°C cold stress. Taken together, the cold resistance of SII-treated Oenothera speciosa plants was improved, which made it possible to be used as a perennial flower in landscaping in Beijing.

Silico Ion Implantation, Oenothera speciosa, Cold Resistant Breeding

Ma, Y. , Lin, Y. , Sun, L. , Song, L. , Cui, R. and Qie, Y. (2015) Cold-Resistant Breeding of Oenothera speciosa Using Silico Ion Implantation. Open Journal of Forestry, 5, 546-554. doi: 10.4236/ojf.2015.55047.