Recently, uncertainty measurement is more and more recognizable in modern data management, conformity assessment, and laboratory accreditation system because of its importance. In this paper, a set of reasonable probability explanations are introduced and an effective method is pro- posed to quantify the assessment indices for the uncertainty measurement of electrical testing laboratory. First of all, the influence from uncertainty factors during the test process is taken into account. With the use of ISO/IEC Guide 98-3 standard and probability theory, the index and model for the measurement uncertainty assessment of a laboratory is then derived. From the simulation results of safety testing, laboratory uncertainty measurement assessment activity for actual electrical appliances, and the confirmation of Monte Carlo simulation method, the appropriateness and correctness of proposed method are verified.