ABSTRACT
Objective: The aim of this
study was correlation proliferative activity, markers express stem cells, and
lipid peroxides of undifferentiated stem cells of human adult dental follicle
(DF) following culture. Methods: For this study, we used 8 samples from DF of
impacted third molars to maintain culture conditions and evaluated the growth
curve, cell viability, production of lipid peroxidation, cell cycle phases, and
proliferative index during 25 days of culture. Results: Cells after culture
showed characteristics of fibroblast-like type following 25th day of culture.
The results of lipid peroxidation showed that stem cells in culture produce 13
nmoles/ml malondialdehyde at the start of culture, increasing until the 12th
day and then began a decline that lasted until the 25th day. We revealed that
DFSCs presented a significantly higher percentage of cells in S + G2/M phases
by the 15th day of culture compared with cells at the start of culture. Cell
surface markers revealed that cell lines were negative for HLA-DR and positive
for CD90, CD44, and CD105. The expression of p21 protein, involved in the
regulation of the cell cycle, showed a significant increase from the 15th to
25th day of culture. Results of cell division rates show a significant increase
between the 6th and 15th day of culture. Conclusions: We conclude that the
culture remained stable during the 25 days of culture, presenting the markers
of stem cells and markers of control, progression, and cell proliferation that
there was an increased production of lipid peroxides between the 6th and 12th
days; this increase is related to the increased numbers of cells that also
occurs during this period. Then, there is a significantly decline in the
production of lipid peroxides and the number of cells, which is accompanied by
an increase in cell unviability.