Author(s)

Xiping Yuan^*, Hong Li, Chongrui Wang, Bo Hong

Fishery Environmental Monitoring Station, Fisheries Research Institute of Hunan Province, Changsha, China.

Approximately 200 mg of fin tissue from each specimen was cut into pieces and treated with a gradient of ethanol (65%, 70%, 75%, 80%, 85%, 90%, 95% and 100%) and then DNA was extracted from formalin fish fin tissues. Agarose gel visualization of the DNA confirmed DNA extracted. MtDNA cyt b gene banding pattern of samples with agarose gel was clearly visible and could be used to be sequenced. In terms of DNA purity, the 260/280 ratio of 87.5% samples ranged between 1.8 and 2.0, indicating that DNA of the majority of the samples was pure. The developed DNA extraction procedure from formalin fish fin tissues by pretreatment with a gradient of ethanol system will be a useful tool to study the genetic structure and phylogenesis of endangered fish, by specimens preserved formalin-fixed in museum and herbarium.

Formalin, Fish Fin, Mitochondria DNA

Yuan, X. , Li, H. , Wang, C. and Hong, B. (2014) DNA Extraction from Formalin Fixed Coilia macrognathos Fin Tissues. Agricultural Sciences, 5, 1097-1099. doi: 10.4236/as.2014.512119.