Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification

HTML  Download Download as PDF (Size: 373KB)  PP. 1-6  
DOI: 10.4236/jst.2014.41001    3,879 Downloads   6,046 Views  Citations

ABSTRACT

The work described herein examines a rapid mix-and-measure method called DETECHIP suitable for screening of steroids and metabolites. The addition of steroids and metabolites to reactive arrays of colorimetric sensors generated characteristic color “fingerprints” that were used to identify the analyte. A color analysis tool was used to identify the analyte pool that now includes biologically relevant analytes. The mix-and-measure arrays allowed the detection of disease metabolites, orotic acid and argininosuccinic acid; and the steroids androsterone, 1,4-androstadiene, testosterone, stanozolol, and estrone. The steroid 1,4-androstadiene was also detected by this method while dissolved in synthetic urine. Some of the steroids, such as androstadiene, stanozolol, and androsterone were co-dissolved with (2-hydroxypropyl)-β-cyclodextrin in order to increase solubility in aqueous buffered solutions. The colorimetric arrays do not intend to eliminate ELISA or mass spectroscopy based screening, but to possibly provide an alternative analytical detection method for steroids and metabolites.

Share and Cite:

Batres, G. , Jones, T. , Johnke, H. , Wilson, M. , Holmes, A. and Sikich, S. (2014) Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification. Journal of Sensor Technology, 4, 1-6. doi: 10.4236/jst.2014.41001.
Journals Menu
Follow SCIRP
Contact us
+1 323-425-8868
customer@scirp.org
WhatsApp +86 18163351462(WhatsApp)
Click here to send a message to me 1655362766
Paper Publishing WeChat

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.