This paper proposes an efficient method for designing accurate structure-specified mixed H₂/H_∞ optimal controllers for systems with uncertainties and disturbance using particle swarm (PSO) algorithm. It is designed to find a suitable controller that minimizes the performance index of error signal subject to an unequal constraint on the norm of the closed-loop system. Although the mixed H₂/H_∞ for the output feedback approach control is considered as a robust and optimal control technique, the design process normally comes up with a complex and non-convex optimization problem, which is difficult to solve by the conventional optimization methods. The PSO can efficiently solve design problems of multi-input-multi-output (MIMO) optimal control systems, which is very suitable for practical engineering designs. It is used to search for parameters of a structure-specified controller, which satisfies mixed performance index. The simulation and experimental results show high feasibility, robustness and practical value compared with the conventional proportional-integral-derivative (PID) and proportional-Integral (PI) controller, and the proposed algorithm is also more efficient compared with the genetic algorithm (GA).