Author(s)

Yohei Fujii, Reiji Tanaka, Hideo Miyake, Yutaka Tamaru, Mitsuyoshi Ueda, Toshiyuki Shibata

Japan Science and Technology Agency, CREST, Tokyo, Japan; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Major of Life Sciences, Graduate School of Bioresources, Mie University, Mie, Japan;.
Major of Life Sciences, Graduate School of Bioresources, Mie University, Mie, Japan;Japan Science and Technology Agency, CREST, Tokyo, Japan.

The antioxidative properties of phlorotannins isolated from the brown alga Eisenia bicyclis were measured using the H-ORAC (Hydrophilic Oxygen Radical Absorbance Capacity) method. The ORAC values of phloroglucinol and it’s oligomers: eckol, fucofuroeckol A, phlorofucofuroeckol A, dieckol, 8,8’-bieckol, were 2.57 ± 0.14, 4.97 ± 0.36, 9.82 ± 0.70, 8.97 ± 0.89, 10.22 ± 0.85, 8.62 ± 0.92 μmol Trolox equivalent/μmol, respectively. With the exception of eckol, the ORAC values of tested phlorotannins were higher than those of the well-known antioxidants (epigallocatechin gallate, resveratrol and L-ascorbic acid) used as positive controls. As a result of comparing with known ORAC values, it was found that the dieckol and fucofuroeckol A had stronger antioxidant activity than representative polyphenols (e.g., kaempferol, quercetin, myricetin and chlorogenic acid) derived from terrestrial plants.

Antioxidants; Brown Alga; Dieckol; Eisenia bicyclis; Fucofuroeckol A; H-ORAC Assay; Phlorotannins

Y. Fujii, R. Tanaka, H. Miyake, Y. Tamaru, M. Ueda and T. Shibata, "Evaluation for Antioxidative Properties of Phlorotannins Isolated from the Brown Alga Eisenia bicyclis, by the H-ORAC Method," Food and Nutrition Sciences, Vol. 4 No. 8A, 2013, pp. 78-82. doi: 10.4236/fns.2013.48A010.