A Novel Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs in Their Tablets and Capsules Using Vanillin Reagent - American Journal of Analytical Chemistry

AJAC > Vol.4 No.3, March 2013

A Novel Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs in Their Tablets and Capsules Using Vanillin Reagent ()

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DOI: 10.4236/ajac.2013.43020 5,439 Downloads 10,306 Views Citations

Author(s)

Mohamed A. El Hamd, Sayed M. Derayea, Osama Hassan Abdelmageed, Hassan F. Askal

Affiliation(s)

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, Egypt.
Pharmaceutical Chemistry Department, Faculty of Pharmacy, King Abdulaziz University, Jeddah, KSA.

ABSTRACT

A selective and new spectrophotometric method is described for determination of five 1,4 dihydropyridine drugs (1,4- DHP); namely nifedipine (NIF), nicardipine (NIC), nimodipine (NIM), felodipine (FEL) and amlodipine (AML). The method is based on a coupling reaction between the cited drugs and vanillin reagent in acidic condition. Under optimized conditions, the red coloured products were measured at 500 nm for NIF, NIC, NIM and FEL or at 479 nm for AML. Molar absorptivities were ranged from 0.575 × 10⁴ － 1.065 × 10⁴ l·mol^-1·cm^-1, Beer’s law was obeyed at 5 - 70 μg/mL concentration range and the limit of detection was ranged from 0.150 - 1.500 μg/mL. The proposed method was successfully extended to pharmaceutical preparations tablets and capsules and comparison by Student’s t-test and variance ratio F-test showed no significant difference.

KEYWORDS

Spectrophotometric Method; 1, 4-DHP; Validated; Selective; Vanillin Reagent

Share and Cite:

M. Hamd, S. Derayea, O. Abdelmageed and H. Askal, "A Novel Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs in Their Tablets and Capsules Using Vanillin Reagent," American Journal of Analytical Chemistry, Vol. 4 No. 3, 2013, pp. 148-157. doi: 10.4236/ajac.2013.43020.

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