Author(s)

Defu Zhi, Shuibao Zhang, Yinan Zhao, Shaohui Cui, Bing Wang, Huiying Chen, Defu Zhi, Defeng Zhao

SEAC-ME Key Laboratory of Biochemistry Engineering, Dalian Nationalities University, Dalian, China.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.
tate Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.

Design and synthesis of a carbamate-linked cationic lipid DDCTMA (N-[1-(2,3-didodecylcarbamoyloxy)propyl]-N,N,N-trimethylammonium iodide)  as gene delivery carriers was described in this work. The transfection efficiency of cationic liposome increased dramatically with the increase in the content of DOPE. In addition, the transfection efficiency of some of cationic lipoplexes was superior or parallel to that of two commercial transfection agents, Lipofectamine2000 and DOTAP. The carbamate-linked cationic lipid DDCTMA/DOPE may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.

Cationic Lipids; DNA Condensation; Gene Delivery; Transfection Efficiency

D. Zhi, S. Zhang, Y. Zhao, S. Cui, B. Wang, H. Chen, D. Zhi and D. Zhao, "In Vitro Study of Carbamate-Linked Cationic Lipid for Gene Delivery Against Cervical Cancer Cells," Advances in Materials Physics and Chemistry, Vol. 2 No. 4B, 2012, pp. 229-232. doi: 10.4236/ampc.2012.24B058.