Author(s)

Yasue Takahashi, Kazunori Shimada, Katsuhiko Sumiyoshi, Takashi Kiyanagi, Makoto Hiki, Kosuke Fukao, Kuniaki Hirose, Rie Matsumori, Hiromichi Ohsaka, Atsumi Kume, Tetsuro Miyazaki, Hiroaki Miyajima, Isao Nagaoka, Hiroyuki Daida

Department of Cardiovascular Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Department of Host Defense and Biochemical Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Division of Cell Biology, Juntendo University Graduate School of Medicine, Tokyo, Japan.

Background: Acute coronary syndrome (ACS) is an amplified state of inflammation and immune reaction. Dendritic cells (DCs) expressing various Toll-like receptors (TLRs) have been observed in atherosclerotic lesions, however, the clinical significance of DCs in pathogenesis of ACS has not been completely investigated. Methods: Ten patients with ACS and 10 patients with stable angina pectoris (SAP) were enrolled in this study. Monocyte-derived DCs were generated from CD14⁺ cells by culturing with granulocyte macrophage colony-stimulating factor and interleukin (IL)-4 for 6 days. Expression of cell surface CD86 and CD83 were measured by flowcytometry. Expression of genes, including CD86, CD83, CCL19, CCR7, TLR2, TLR4, TLR5, TLR8, and TLR9, were measured by real-time PCR. Plasma IL-6 and tumor necrosis factor (TNF)-α levels were also measured. Results: The number of CD86⁺CD83⁺DCs in the ACS group was significantly higher than that in the SAP group (P < 0.05). Expression levels of CD86, CD83, CCL19, CCR7, TLR2, TLR4, and TLR9 in the ACS group were significantly higher than those in the SAP group (all, P < 0.05). Plasma IL-6 and TNF-α levels in the ACS group were significantly higher than those in the SAP group (all, P < 0.05). In addition, a positive correlation was observed between the number of CD86⁺CD83⁺ cells and plasma levels of IL-6 (P = 0.88, P < 0.0001) as well as between the number of CD86⁺CD83⁺ cells TNF-α levels (r = 0.78, P < 0.0001). Conclusions: These results demonstrated that mono-cyte-derived DCs are activated in patients with ACS, suggesting that activated DCs may play an important role in the pathogenesis of ACS.

Acute Coronary Syndrome (ACS); Dendritic Cells (DCs); Toll-Like Receptors (TLRs)

Takahashi, Y. , Shimada, K. , Sumiyoshi, K. , Kiyanagi, T. , Hiki, M. , Fukao, K. , Hirose, K. , Matsumori, R. , Ohsaka, H. , Kume, A. , Miyazaki, T. , Miyajima, H. , Nagaoka, I. and Daida, H. (2012) Clinical significance of monocyte-derived dendritic cell activation in patients with acute coronary syndrome. World Journal of Cardiovascular Diseases, 2, 74-81. doi: 10.4236/wjcd.2012.22012.