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Dr. Levent Keskintepe

Medical College of Nevada

Department of Gynecology and Obstetrics, USA

Clinical Professor

Email: Levent.keskintepe@integramed.com


2006 Professor, Dept. of OBGYN, Medical College of Georgia

2004 HCLD, CC, American Association of Bioanalysts

1994 Ph.D., Department of Physiology and Pharmacology, School of Veterinary Medicine, University of Georgia, USA

1984 D.V.M School of Veterinary Medicine, Firat University, Turkey

Research Fields

In vitro maturation


culture Oocyte/Embryo/Sperm cryopreservation Preimplantation genetic screening

Publications (selected)

  1. Bagis H., Keskintepe L., Odaman H. Sagirkaya H., Cetin S. (2001). Influences of cumulus cells during in vitro fertilization of mouse oocytes in different mouse strain. Turkish Journal of Veterinary and Animal Science, 25:777-782.
  2. Bagis H., Keskintepe L., Odaman H., Sagirkaya H., Sekmen S. (2002). Comparing the birth rates of microinjected hybrid mouse zygotes transferred into outbreed and hybrid foster mothers. Turkish Journal of Veterinary and Animal Science, 26:210-216.
  3. Keskintepe L., Pacholczyk G.A., Machnicki A, Norris K., Curuk A., Khan I, and Brackett B.G. (2002). Bovine blastocyst development from oocytes injected with freeze-dried spermatozoa. Biol Reprod 2002 67: 409-415.
  4. Sirisathien S, Keskintepe L, Brackett B. (2003). Bull Sperm Uptake of Exogenous DNA and Efforts to Obtain Transgenic Embryos. Transgenics 4 (1): 23-28.
  5. Fisch JD, Sher G, Adamowicz A, Keskintepe L (2003). The graduated embryo score (GES) predicts ART outcome better than a single day 3 evaluation and achieves results associated with blastocyst transfer from day 3–ET. Fert Ster. 80(6): 1352-1358.
  6. Sher G, Keskintepe L, Nouriani M, Roussev R, Batzofin J (2004). Expression of sHLA-G in supernatants of individually cultured 46-h embryos: a potentially valuable indicator of 'embryo competency' and IVF outcome. Reproductive BioMedicine Online Vol. 9, No. 1. 74–78.
  7. Keskintepe. L (2004). Assessing sHLA-G secretion: a useful marker for embryo selection on day 3. Fertility World 2:12.
  8. Sher G, Keskintepe L, Fisch JD, Acacio BA, Ahlering, P, Batzofin J (2005). Embryo selection for transfer on the basis sHLA-G expression 46 hours post-ICSI significantly enhances IVF implantation and overall pregnancy rates. Fertility and Sterility. 83(5):1410-3.
  9. Sher G, Keskintepe L, Fisch JD, Acacio BA, Ahlering, P, Batzofin J (2005). Influence of Early ICSI-derived Embryo sHLA-G Expression on Pregnancy and Implantation Rates: a prospective study. Human Reproduction 20(5):1359-63.
  10. Sher G, Keskintepe L, Ginsburg M (2007). sHLA-G expression: is it really worth measuring? RBM Online 2007 14(1) 9–10.
  11. Fisch JD, Keskintepe L, Ginsburg M, Adamowicz M, Sher G (2007). Graduated Embryo Score and soluble human leukocyte antigen-G expression improve assisted reproductive technology outcomes and suggest a basis for elective single-embryo transfer. Fertil Steril. 87(4):757-63.
  12. Sher G, Keskintepe L., Keskintepe M., Ginsburg M., Maassarani G., Yakut T., Baltaci V., Kotze D., Unsal E. (2007). Oocyte Karyotyping by Comparative Genomic Hybridization Provides a Highly Reliable Method for Selecting “Competent” Embryos, Fertility and Sterility Fertil Steril.87(5):1033-40.
  13. Fisch JD, Keskintepe L, Sher G. (2007). Gonadotropin releasing hormone agonist/antagonist conversion with estrogen priming: a novel approach for improving ART outcomes in poor-responders. Fertil Steril. 2008 Feb;89(2):342-7.
  14. Keskintepe L, Sher G, Keskintepe M (2007). Reproductive oocyte/embryo karyotyping: a comparison between fluorescence in situ hybridization (FISH) versus comparative genomic hybridization (CGH). Fertil Steril. 87(5):1033-40.
  15. Keskintepe L, Sher G, Keskintepe M. (2007). Reproductive oocyte/embryo genetic analysis: comparison between fluorescence in-situ hybridization and comparative genomic hybridization. Reprod Biomed Online. 15 (3):303-9.
  16. Keskintepe L, Norris K, Pacholczyk G, Dederscheck SM, Eroglu A. (2007). Derivation and comparison of C57BL/6 embryonic stem cells to a widely used 129 embryonic stem cell line. Transgenic Res. 2007 Dec; 16 (6):751-8.
  17. Sher G, Keskintepe L, Mukaida T, Keskintepe M, Ginsburg M, Agca Y, Maassarani G, Bayrak A. (2008). Selective vitrification of euploid oocytes markedly improves survival, fertilization and pregnancy-generating potential. Reprod Biomed Online. 17 (4):524-9.
  18. Keskintepe L, Agca Y, Sher G, Keskintepe M, Maassarani G. (2009). High survival rate of metaphase II human oocytes after first polar body biopsy and vitrification: determining the effect of previtrification conditions. Fertility and Sterility 92(5); 1706-1715.
  19. Sher G, Keskintepe L, Keskintepe M, Maassarani G, Tortoriello D, Brody S. (2009). Genetic analysis of human embryos by metaphase comparative genomic hybridization (mCGH) improves efficiency of IVF by increasing embryo implantation rate and reducing multiple pregnancies and spontaneous miscarriages. Fertil Steril. 92 (6); 1886-1894.
  20. Keskintepe L, Sher G, Machnicka A, Tortoriello D, Bayrak A, Fisch J, Agca Y (2009). Vitrification of Human Embryos Subjected to Blastomere Biopsy for Pre-implantation Genetic Screening Produces Higher Survival and Pregnancy Rates than Slow Freezing. J Assist Reprod Genet. 26 (11-12):629-35.
  21. Kotze DJ, Hansen P, Keskintepe L, Snowden E, Sher G, Kruger T. (2010). Embryo selection criteria based on morphology versus the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome. J Assist Reprod Genet. PMID: 20358276.
  22. Keskintepe L and Beyhan Z (2010). Intracytoplasmic Sperm Injection (ICSI) and the factors affecting the immediate outcome: fertilization and preimplantation development. J. Reprod. Stem Cell Biotechnol. 1(2) 193-211, 2010.
  23. Kotze D, Kruger TF, Lombard C, Padayachee T, Keskintepe L, Sher G. (2013). The effect of the biochemical marker soluble human leukocyte antigen G on pregnancy outcome in assisted reproductive technology--a multicenter study. Fertil Steril. 2013 Nov; 100(5):1303-9.
  24. Keskintepe. L and Eroglu A. (2015). Freeze-Drying of Mammalian Sperm. Willem F. Wolkers and Harriette Oldenhof (eds.), Cryopreservation and Freeze-Drying Protocols, Methods in Molecular Biology, vol. 1257, DOI 10.1007/978-1-4939-2193-5_25, Springer Science+Business Media New York 2015.
  25. Tortoriello DV, Dayal M, Beyhan Z, Yakut T, Keskintepe L. Reanalysis of human blastocysts with different molecular genetic screening platforms reveals significant discordance in ploidy status. J Assist Reprod Genet. 2016 Jul 16....