Radioiodination of Cephalexin with 125I and Its Biological Behavior in Mice
A.Abd El-Bary, A.M. Amin, A. El-wetery, S. Saad, M. Shoukry
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DOI: 10.4236/pp.2012.31014   PDF    HTML     3,647 Downloads   7,234 Views   Citations

Abstract

A procedure for radioiodination of Cephalexin with iodine-125 was carried out via an electrophilic substitution reaction. Using 3.7 MBq of Na125I, 200μl of cephalexin as substrate, 200 μl of iodogen as oxidizing agent in acetone at 40°C for 20 min, a maximum radiochemical yield of 125I-Ceph (95%) was obtained. The labeled compound was separated and purified by means of high-pressure liquid chromatography (HPLC). The biological distribution in normal and inflamed (septic and aseptic) mice indicates the suitability of radioiodinated cephalexin for imaging of inflammation

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A. El-Bary, A. Amin, A. El-wetery, S. Saad and M. Shoukry, "Radioiodination of Cephalexin with 125I and Its Biological Behavior in Mice," Pharmacology & Pharmacy, Vol. 3 No. 1, 2012, pp. 97-102. doi: 10.4236/pp.2012.31014.

Conflicts of Interest

The authors declare no conflicts of interest.

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