Antimicrobial Activity and Rates of Tannins in Stryphnodendron adstringens Mart. Accessions Collected in the Brazilian Cerrado

Giovana Maria Lanchoti Fiori, Ana Lucia Fachin, Valéria S. C. Correa, Bianca Waleria Bertoni, Silvana Giuliatti, Saulo França Amui, Suzelei de Castro França, Ana Maria Soares Pereira

Department of Genetics, University of S?o Paulo—USP, Ribeir?o Preto, Brazil.
Reserve EcoCerrado Brazil, Araxá, Brazil.
University of Ribeir?o Preto—UNAERP, Ribeir?o Preto, Brazil.

DOI: 10.4236/ajps.2013.411272                

Abstract

Inner bark extracts of Stryphnodendron adstringens, a leguminous tree species known as Barbatimão, are often incorporated to phytotherapic formulations due to their antimicrobial and healing activities. In this study, extracts from S. adstringens accessions collected in 12 distinct locations were investigated in order to determine the rates of tannins in inner barks and to validate S. adstringens antibacterial and antifungal effectiveness. Yields of tannins were quantified by colorimetric assay following methodology described in the Brazilian Pharmacopoeia and the antimicrobial activity was determined by microdilution technique proposed by the National Committee for Clinical Laboratory Standards using S. adstringens hydroalcoholic and aqueous extracts and semi-purified fractions. Investigated extracts did not present significant antibacterial activity though aqueous extracts exhibited antifungal effect against both Trichophyton rubrum mutant and clinical strains (MIC 156 μg/mL). A positive correlation between tannin concentration and antifungal activity was observed and the accessions collected in Delfinópolis (MG) were considered elite.

Keywords

Cerrado; Barbatimão; Genetic Diversity; Phytoterapy

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	S. Panizza, “Plants that Heal—Smell of the Woods,” Ibrasa, São Paulo, 1998.
[2]	G. C. Lopes, C. C. A. Sanches and C. V. Nakamura, “Influence of Extracts of Stryphnodendron polyphyllum Mart and Stryphnodendron obovatum Benth. On the Cicatrisation of Cutaneous Wounds in Rats,” Journal of Ethnopharmacology, Vol. 99, No. 2, 2005, pp. 265-272.
[3]	E. A. Audi, D. P. Toledo, P. G. Peres, E. Kimura, W. K. V. Pereira, J. C. P. De Mello, C. Nakamura, W. Alves-Do-Prado, C. A. Cuman and C. A. Bersani-Amado, “Gastric Antiulcerogenic Effects of Stryphnodendron adstringens in Rats,” Phytotherapy Research, Vol. 13, No. 3, 1999, pp. 264-266.
[4]	D. G. Minatel, A. M. S. Pereira, T. M. Chiaratti, L. Pasqualin, J. C. N. Oliveira, L. B. Couto, R. C. C. Lia, J. M. Cintra, M. F. A. Bezzon and S. C. Franca, “Clinical Study for the Validation of the Efficacy of Ointment Containing Barbatimao (Stryphnodendron adstringens (Mart.) Coville on Healing of Decubitus Ulcers,” Brazilian Journal of Medicine, Vol. 67, 2010, pp. 250-256.
[5]	J. C. S. Lima, D. T. Martins and J. R. P. T. Souza, “Experimental Evaluation of Stem Bark of Stryphnodendron adstringens (Mart.) Coville for Anti-Inflammatory Activity,” Phytotherapy Research, Vol. 12, No. 3, 1998, pp. 218-220.
[6]	J. O. Melo, T. H. Endo, L. E. Bersani-Amado, A. E. Svidzinski, S. Baroni, J. C. P. Mello and C. A. Bersani-Amado, “Effect of Stryphnodendron adstringens (Barbatimão) Bark on Animal Models of Nociception,” Brazilian Journal of Pharmaceutical Sciences, Vol. 43, No. 3, 2007, pp. 465-469. http://dx.doi.org/10.1590/S1516-93322007000300015
[7]	B. Holetz, T. Ueda-Nakamura, B. P. Dias Filho, J. C. Mello, J. A. Morgadodiaz, C. E. Toledo and C. V. Nakamura, “Biological Effects of Extracts Obtained from Stryphnodendron adstringens on Herpetomonas samuelpessoai,” Memories of the Oswaldo Cruz Institute, Vol. 100, No. 4, 2005, pp. 397-401. http://dx.doi.org/10.1590/S0074-02762005000400010
[8]	I. Ishida, J. C. De Mello, D. A. G. Cortez, B. P. Filho, T. Uedanakamura and C. V. Nakamura, “Influence of Tannins from Stryphnodendron adstringens on Growth and Virulence Factor of Candida albicans,” Journal of Antimicrobial Chemotherapy, Vol. 58, No. 5, 2006, pp. 942-949. http://dx.doi.org/10.1093/jac/dkl377
[9]	C. Seebacher, J.-P. Bouchara and B. Mignon, “Updates on the Epiodermiology of Dermatophyte Infections,” Mycopathologia, Vol. 166, No. 5-6, 2008, pp. 335-352. http://dx.doi.org/10.1007/s11046-008-9100-9
[10]	A. L. Fachin, E. P. Contel and N. M. Martinez-Rossi, “Effect of Sub-MICs of Antimycotics on Expression of Intracellular Esterase of Trichophyton rubrum,” Medical Mycology, Vol. 39, No. 1, 2001, pp. 129-133. http://dx.doi.org/10.1080/mmy.39.1.129.133
[11]	A. C. Andrade, L.-H. Zwiers and M. A. de Waard, “Pesticide Chemistry and Bioscience,” G. T. Brooks and T. R., Eds., The Food-Environment Challenge, Royal Society of Chemistry, Cambridge, 1999, pp. 221-235. http://dx.doi.org/10.1533/9781845698416.5.221
[12]	A. L. Fachin, M. S. Ferreira-Nozawa, J. R. W. Maccheroni and N. M. Martinez-Rossi, “Role of the ABC Transporter TruMDR2 in Terbinafine, 4-Nitroquinoline-N-Oxide (4NQO) and Ethidium Bromide Resistance in Trichophyton rubrum,” Journal of Medical Microbiology, Vol. 55, No. 8, 2006, pp. 1093-1099. http://dx.doi.org/10.1099/jmm.0.46522-0
[13]	National Committee for Clinical Laboratory Standards, “Reference Method for Broth Dilution Antifungal Susceptibility Testing of Conidium-Forming Filamentous Fungi,” Proposed Standard, NCCLS Document M38-A, National Committee for Clinical Laboratory Standards, Wayne, 2002.
[14]	National Committee for Clinical Laboratory Standards, “Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically,” Approved Standard M7-A2, National Committee for Clinical Laboratory Standards, Villanova, 1990.
[15]	Brazil. Ministry of Health National Health Surveillance Agency, “Resolution RDC 49, 23 November 2010. Approves the Brazilian Pharmacopoeia,” 5th Edition, Official Gazette, Brasília, 2010.
[16]	V. R. Santos, R. T. Gomes, R. R. Oliveira, M. E. Cortes and M. G. L. Brandão, “Susceptibility of Oral Pathogenic Microorganisms to Aqueous and Ethanolic Extracts of Stryphnodendron adstringens (Barbatimão),” International Journal of Dentistry, Vol. 8, No. 1, 2009, pp. 1-5.
[17]	C. Serafin, V. Nart, A. Malheiros, A. B. Cruz, F. D. Monache, M. A. Gette, S. Zacchino and V. Cechinel Filho, “Evaluation of the Antimicrobial Effects of Plinia glomerata (Myrtaceae),” Brazilian Journal of Pharmacognosy, Vol. 17, No. 4, 2007, pp. 578-582.
[18]	T. K. B. Jacobson, J. Garcia, S. C. Santos, J. B. Duarte, J. G. Farias and H. J. Kliemann, “Influence of Edaphic Factors in the Production of Total Phenols and Tannins Two Barbatimão Species (Stryphnodendron sp.),” Tropical Agricultural Research, Vol. 35, No. 3, 2005, pp. 163-169.
[19]	D. A. Oliveira, E. C. S. Junior, C. Castilho, J. V. Pereira, R. C. L. Pietro and M. D. Souza Neto, “Evaluation of the Antimicrobial Activity of Solutions of Sodium Hypochlorite at Different Concentrations and Barbatimão,” Journal Brazilian Phytomedine, Vol. 3, 2005, pp. 5-12.