Effects of dietary CoQ10 and α-lipoic acid on CoQ10 levels in plasma and tissues of eggs laying hens


In this paper we described the effect of administrated CoQ10, and alfa-lipoic acid on the concentration of total CoQ10 inplasma end body tissues of eggs laying hens. Organisms raise a complex network of enzymes, metabolites and molecules with antioxidant activities in order to prevent oxidative damage of theirs bodies. Adequate blood concentrations of small weight molecules ingested with food and food additives are important for the proper functioning of the antioxidant defense. To test this hypothesis we prepared following experiment. Forty weeks old hens were selected from two genotypes; Ross 308 broiler mothers and Lohmann breed hens. Animals were fed for a period of 84 days. Concentrations of supplemented CoQ10 and ALAwere calculated from feed instruction tables so each hen received an average of approximately 5 mg of CoQ10 and 50 mg ofALAper kg of animal weight per day. During the experiment blood samples were taken and at the end of the experiment different body tissues (heart, liver, breast, legs) were collected and analyzed with originally developed HPLC-MS/MS method based selective ionization with LiCl on MRM scanning. We found a number of interesting and unexpected results. Supplemented CoQ10 increased concentrations of coenzyme CoQ10 inplasma and different hen’s tissues. Increased concentration of CoQ10 is the result of its transfer with chylomicrons from the digestive tract to various organs of the body and to the liver where exogenous and endogenous CoQ10 has been re-redistributed through lipoproteins. Supplemented ALA caused much greater concentration of CoQ10 indifferent tissues and plasma then CoQ10. Plausible explanation of our results is such that ALA may regenerates the antioxidants and accelerate the formation of endogenous CoQ10 which is distributed with lipoprotein carriers and increases overall concentration of CoQ10. Our experiments definitely show that Lipoic acid beside glutathione promotes also a synthesis of CoQ10 and increases the total concentration especially in liver and heart tissues.

Share and Cite:

Krizman, P. , Smidovnik, A. , Wondra, A. , Krizman, M. and Prosek, M. (2013) Effects of dietary CoQ10 and α-lipoic acid on CoQ10 levels in plasma and tissues of eggs laying hens. Journal of Biomedical Science and Engineering, 6, 185-191. doi: 10.4236/jbise.2013.62022.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Davies, K.J. (1995) Oxidative stress: The paradox of aerobic life. Free Radicals and Oxidative Stress: Envi ronment, Drugs and Food Additives, 61, 1-31.
[2] Halliwell, B. (2006) Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiology, 141, 312-322. doi:10.1104/pp.106.077073
[3] Sies, H. (1997) Oxidative stress: Oxidants and antioxidants. Experimental Physiology, 82, 291-295.
[4] Packer, L. and Colman, C. (1999) The antioxidant miracle. John Wiley & Sons, New York, 1-30.
[5] Schafer, F.Q. and Buettner, G.R. (2001) Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radical Biology and Medicine, 30, 1191-1212. doi:10.1016/S0891-5849(01)00480-4
[6] Jones, D.P. (2006) Redefining oxidative stress. Antioxidants & Redox Signaling, 8, 1865-1879. doi:10.1089/ars.2006.8.1865
[7] Kemp, M., Go, Y.M. and Jones, D.P. (2008) Nonequilibrium thermodynamics of thiol/disulfide redox systems: A perspective on redox systems biology. Free Radical Biology and Medicine, 44, 921-937. doi:10.1016/j.freeradbiomed.2007.11.008
[8] Jazbec-Krizman, P., Smidovnik, A., Golc-Wondra, A., Cernelic, K., Kotnik, D., Krizman, M., Prosek, M., Volk, M., Holcman, A., and Nemec-Svete, A. (2012) Quantitative determination of low molecular weight antioxidants and their effects on different antioxidants in chicken blood plasma, Journal of Biomedical Science and Engineering, 5, 743-754. doi:10.4236/jbise.2012.512093
[9] Jazbec-Krizman, P., Prosek, M., Smidovnik, A., Golc Wondra, A., Glaser, R., Vindis-Zelenko, B., and Volk, M. (2012) Products with increased content of CoQ10 pre pared. In: Hafiz, A. and Eissa, A., Editors. Chickens Fed with Supplemental CoQ10. http://ebookee.org/Trends-in-Vital-Food-and-Control-Engineering
[10] Kotnik, D., Jazbec-Krizman, P., Krizman, M., Zibert, T., Smidovnik, A. and Prosek, M. (2013) Rapid and sensitive HPLC-MS/MS method for quantitative determination of CoQ10, Journal of Research on Precision Instrument and Machinery. (in press)
[11] Littarru, G.P., Mosca, F., Fattorini, D., Bompadre, S. and Battino, M. (2004) Assay of coenzyme Q10 in plasma by a single dilution step. Methods in Enzymology, 378, 170 176. doi:10.1016/S0076-6879(04)78014-3
[12] Lohmann Brown Management Guide (2007). www.stonegate.co.uk/pdfs/lohmann_management.pdf
[13] http://en.aviagen.comasse/assest/Tech_Center./Ross_PS/Ross-308-PS-PO-2011.pdf
[14] Prosek, M., Butinar, J., Lukanc, B., Milivojevic-Fir, M., Milivojevic, L., Krizman, M. and Smidovnik, A. (2008) Bio-availability of water-soluble CoQ10 in beagle dogs. Journal of Pharmaceutical and Biomedical Analysis, 47, 918-922. doi:10.1016/j.jpba.2008.04.007
[15] Packer, L., Witt, E.H. and Tritschler, H.J. (1995) Alpha lipoic acid as a biological antioxidant. Free Radical Biology and Medicine, 19, 227-250. doi:10.1016/0891-5849(95)00017-R
[16] Han, D., Tritschler, H.J. and Packer, L. (1995) Lipoic acid increases intracellular glutathione in a human T-lym phocyte Jurkat cell line. Biochemical and Biophysical Research Communications, 207, 258-264. doi:10.1006/bbrc.1995.1181
[17] Han, D., Handelman, G., Marcocci, L., Sen, C.K., Roy, S., Kobuchi, H., Tritschler, H.J., Flohe, L. and Packer, L. (1997) Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization. BioFactors, 6, 321-338. doi:10.1002/biof.5520060303
[18] Bilska, A. and Wlodek, L. (2005) Lipoic acid—The drug of the future? Pharmacological Reports, 57, 570-577.
[19] Smith, A.R., Shenvi, S.V., Widlansky, M., Suh, J.H. and Hagen, T.M. (2004) Lipoic acid as a potential therapy for chronic diseases associated with oxidative stress. Current Medicinal Chemistry, 11, 1135-1146. doi:10.2174/0929867043365387

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.