Evaluation of pathogencity and race classification of Xanthomonas oryzae pv. oryzae in guilan province-Iran


To evaluation of pathogenicity and race classification of Xanthomonas oryzae pv. oryzae agent bacterial leaf blight of rice, 153 isolates of X. oryzae pv. oryzae were collected from different rice-growing cities of Guilan province – Iran. All of isolates were inoculated to assess the differential characteristics of 26 near isogenic rice lines containing a single resistance gene or two to five genes. Inoculation was done 21 days after sowing in the greenhouse. Scoring of inoculated plants was done 18 days after inoculation. The level of infection was not so clear among pyramiding lines, expect IRBB53 and IRBB61. Therefore, the pyramiding lines can not be used as differentials for pathogenicity evaluation of X. oryzae pv. oryzae The 12 rice lines with a single resistance gene were used further to establish a system of races classification of X. oryzae pv. oryzae IRBB14, IRBB21and IRBB7 were resistance to the most isolates. Whereas, IRBB1, IRBB2, IRBB4 and IRBB10 were susceptible to all isolates. Based on the interactions between the isolates X. oryzae pv. oryzae and the 12 near-isogenic rice lines, seven singlegene rice lines were chosen as differentials, and the 153 tested isolates were classified into four races. Except for cultivar types, different terrain, climate, period of rice planting and other factors may be associated with the population diversity and virulent variation of X. oryzae pv. oryzae.

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Khoshkdaman, M. , Ebadi, A. and Kahrizi, D. (2012) Evaluation of pathogencity and race classification of Xanthomonas oryzae pv. oryzae in guilan province-Iran. Agricultural Sciences, 3, 557-561. doi: 10.4236/as.2012.34066.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Swings, J., Vander Mooter, M., Vauterin, L., Hoste, B., Gillis, M., Mew, T.W. and Kersteres, K. (1990) Reclassification of the causal agents of bacterial blight (Xanthomonas campestris pv. oryzicola) of rice as pathovars of Xanthomonas oryzae (ex Ishiyama, 1922) sp. nov., nom. rev. International Journal of Systematic Bacteriology, 40, 309-311.
[2] Mew, T.W. (1987) Current status and future prospects of research on bacterial blight of rice. Annual Review of Phytopathology, 25, 359-382. doi:10.1146/annurev.py.25.090187.002043
[3] Mew, T.W. (1993) Xanthomonas oryzae pathovars on rice, cause of bacterial blight and bacterial leaf streak. In: Swings, J.G. and Civerolo, F.L., Eds., Xanthomonas, Chapman & Hall, Lpndon, 30-40.
[4] Ryba-White, M., Notteghem, J.L. and Leach, J.E. (1995) Comparison of Xanthomonas oryzae pv. oryzae strains from Africa, north America and Asia by RFLP analysis. International Rice Research Notes, 20, 25-26.
[5] Ou, S.H. (1985) Rice diseases. Common wealth Mycological Institute, Surrey.
[6] Khoshkdaman, M., Niknejad kazmpour, M., Ebadi, A.A. and Pedramfar, H. (2009) Identification of causal agent of bacterial bight of rice in the fields of Guilan province. Plant Protection Journal, 23, 50-57.
[7] Chu, Z.H., Fu, B.Y., Li, Z.K., Zhang, Q.F. and Wang, S.P. (2004) Targeting the recessive rice gene, Xa13, for bacterial blight resistance to a 14.8-kb DNA fragment. The fourth international crop science congress. http,//www.cropscience.org.au/icsc2004/poster/3/2/1/index.htm
[8] Huang, N., Angeles, E.R., Domingo, J., Magpantay, G., Singh, S., Zhang, G., Kumaravadivel, N., Bennett, J. and Khush, G.S. (1997) Pyramiding of bacterial blight resistance genes in rice, marker-assisted selection using RFLP and PCR. Theoretical and Applied Genetics, 95, 313-320. doi:10.1007/s001220050565
[9] Khush, G.S., Mackill, D.J. and Sindhu, G.S. (1989) Breed- ing rice resistance to bacterial blight. In: Ogawa, T. and Khush, G.S., Eds., Bacterial Blight of Rice, Manila, Philippines. International Rice Research Institute, Los Ba?os, 207-217.
[10] Ogawa, T., Yamamoto, T., Khush, G.S. and Mew, T.W. (1991) Breeding of near-isogenic lines of rice with single genes for resistance to bacterial blight pathogen (Xanthomonas campestris pv. oryzae). Japanese Journal of Breeding, 41,523-529.
[11] Sun, X.L., Cao, Y.L., Yang, Z.F., Xu, C.G., Li, X.H., Wang, S.P. and Zhang, Q.F. (2004) Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein. The Plant Journal, 37,517-527. doi:10.1046/j.1365-313X.2003.01976.x
[12] Jeung, J.U., Heu, S.K., Shin, M.S., Cruz, C.M.V. and Jena, K.K. (2006). Phytopathology, 96, 867-875. doi:10.1094/PHYTO-96-0867
[13] Mew, T.W., Cruz, C.M.V. and Medalla, E.S. (1992). Changes in the race frequency of Xanthomonas oryzae pv. oryzae in response to rice cultivars planted in the Philippines. Plant Disease, 76, 1029-1032. doi:10.1094/PD-76-1029
[14] Adhikari, T.B., Mew, T.W. and Teng, P.S. (1994) Phenotypic diversity of Xantho-monas oryzae pv. oryzae in Nepal. Plant Disease, 78, 68-72. doi:10.1094/PD-78-0068
[15] Liu, H., Yang, W., Hu., B. and Liu, F. (2007). Virulence analysis and race classification of Xanthomonas oryzae pv. oryzae in China. Journal of Phytopathology, 155, 129-135. doi:10.1111/j.1439-0434.2007.01197.x
[16] Fang, Z.D., Xu, Z.G., Guo, C.J., Chen, Y.L., Deng, X.R., Li, H.S. and Zhu, L.M. (1981) Variability of pathogenicity of rice bacterial leaf blight organism. Journal of Nan-jing Agricultural College, 1, 1-11.
[17] Kauffman, H.E., Reddy, A.P.K., Hsieh, S.P.Y. and Merca, S.D. (1973) An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Disease Reporter. 57, 537-541.
[18] International Rice Research Institute (1996). Standard Evaluation System for Rice. 4th Edition, IRRI, Los Ba?os.
[19] Adhikari, T.B., Basnyat, R.C. and Mew, T.W. (1999) Virulence of Xanthomonas oryzae pv. oryzae on rice lines containing single resistance genes and gene combinations. Plant Disease, 83, 46-50. doi:10.1094/PDIS.1999.83.1.46
[20] Mew, T.W., Alvarez, A.M., Leach. J.E. and Swings. J. (1993) Focus on bacterial blight of rice. Plant Disease, 77, 5-12. doi:10.1094/PD-77-0005
[21] Mazzola, M., Leach, J.E., Nelson, R. and White, F.F. (1994) Analysis of the interaction between Xanthomonas oryzae pv. oryzae and the rice cultivars IR24 and IRBB21. Phytopathology, 84, 392-397. doi:10.1094/Phyto-84-392
[22] Ardales, E.Y., Leung, H., Vera Cruz, C.M., Leach, J.E., Mew, T.W. and Nelson, R.J. (1996). Hierarchical analysis of spatial variation of the rice bacterial blight pathogen across agroe-cosystems in the Phillippines. Phytopathology, 86, 241-252. doi:10.1094/Phyto-86-241

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