Influence of MnTE-2-PyP on Inflammation and Lipid Peroxidation in Mouse Asthma Model

Lyudmil Terziev, Veneta Shopova, Violeta Dancheva, Galya Stavreva, Milena Atanasova, Angelina Stoyanova, Tzvetan Lukanov, Anelia Dimitrova

Sector of Biology, Medical University, Pleven, Bulgaria.
Sector of Chemistry, Medical University, Pleven, Bulgaria.
Sector of Clinical Immunology and Allergology, Medical University, Pleven, Bulgaria.
Sector of Disaster Medicine, Medical University, Pleven, Bulgaria.
Sector of Experimental and Clinical Pharmacology, Medical University, Pleven, Bulgaria.
Sector of Pathophysiology, Medical University, Pleven, Bulgaria.

DOI: 10.4236/ojrd.2012.22006                

Abstract

Our aim was to investigate the effects of MnTE-2-PyP on some markers of inflammation and lipid peroxidation in mouse asthma model. 24 female mice were divided into four groups: group 1, controls; group 2, injected with ovalbumin (OVA); group 3, treated with MnTE-2-PyP; and group 4, treated with ovalbumin and MnTE-2-PyP. The mice from groups 2 and 4 were injected with 10 μg OVA and 1 mg Imject Alum^? in 100 μL phosphate buffered saline (PBS) on days 0 and 14. The animals from groups 1 and 3 were injected with 100 μL PBS + Imject Alum^? (1:1). The animals from groups 2 and 4 were subjected to a 30 min aerosol challenge of 1% ovalbumin on days 24, 25 and 26 and those from groups 1 and 3 were subjected to aerosol challenge of PBS at the same time and duration. One hour before inhalation, and 12 hours later the animals from groups 3 and 4 were injected with 100 μL MnTE-2-PyP solution in PBS containing 5 mg/kg. The total cell number, total protein content and 8-isoprostane, IL-4 and IL-5 levels in the bronchialveolar lavage fluid increased in group 2 as compared to the control group. Malone dialdehyde content in the lung homogenate and IgE levels in the serum also increased in this group. The total cell number, total protein content, and levels of 8-isoprostane, IL-4, IL-5 and IgE decreased significantly in group 4 as compared to the OVA group. The parameters set out above in group 3 did not differ significantly from those of the control group. MnTE-2-PyP administered intraperitoneally, 48 hours after the last nebulization, reduced the inflammation and lipid peroxidation in mouse asthma model.

Keywords

Asthma; Inflammation; Interleukins; 8-Isoprostane; Lipid Peroxidation; MnTE-2-PyP

Conflicts of Interest

The authors declare no conflicts of interest.

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