Comparative Evaluation of a Modified Acetic Method for Extraction of Antioxidant Compounds from Black Beans (Phaseolus vulgaris)


Extraction of phenolic compounds from bean seed coats by acetic acid was evaluated and compared to conventional extraction with 80% methanol. Total phenols, flavonoids and free radical scavenging capacity were determined by colorimetric methods. Furthermore, qualitative flavonoid characterization was performed via HPLC diode array. The acetic acid extract contained 73.4 ± 7 mg/g of sample expressed as gallic acid equivalents (GAE), and 41.3 ± 4 mg/g as catechin equivalents (CE). The acetic acid extract (at 1 mg/mL) showed over 89% efficiency to scavenge the 1,1 diphenyl-2-picrylhydrazil (DPPH) radical (EC50 = 6.13 mg extract/mg standard). Gallic acid, delphinidin-3-glycoside, petunidinglycoside, petunidin, malvidin-3-glycoside, myricetin-glycoside, quercitin-glycoside, saponin (β-g), and 3-hydroxy-5, 7-megastigmadien-9-one were identified in both extracts. Additionally, kaempferol-3-glycoside was found in the acetic acid extract. Sequential acetic acid extractions indicated that the antiradical activity was about 90% in DPPH radical scavenging within the first 8 h of extraction, when compared to a 24 h extraction. In general, acetic acid offered a faster and more efficient approach for the extraction of phenolic

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J. Islas, A. Dávalos-Balderas, N. Arroyo-Currás, B. Cano, P. Galindo-Jacobo, G. Guajardo-Salinas, A. Gaytan-Ramos and J. Moreno-Cuevas, "Comparative Evaluation of a Modified Acetic Method for Extraction of Antioxidant Compounds from Black Beans (Phaseolus vulgaris)," Food and Nutrition Sciences, Vol. 3 No. 3, 2012, pp. 348-353. doi: 10.4236/fns.2012.33050.

Conflicts of Interest

The authors declare no conflicts of interest.


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