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Assessment of Posidonia Oceanica (L.) Delile conservation status by standard and putative approaches: the case study of Santa Marinella meadow (Italy, W Mediterranean)

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DOI: 10.4236/oje.2011.12006    6,053 Downloads   13,327 Views   Citations

ABSTRACT

The conservation status of the Posidonia oceanica meadow at Santa Marinella (Rome) was evaluated through both standard (bed density, leaf biometry, "A" coefficient, Leaf Area Index, rhizome production) and biochemical/genetic approaches (total phenol content and Random Amplified Polymorphic DNA marker). The bio-chemical/genetic results are in agreement with those obtained by standard approaches. The bed under study was ranked as a disturbed one, due to its low density, and high heterogeneity in leaf biometry, LAI values, "A" coefficient and primary production. This low quality ranking is confirmed by both mean phenol content in plants, quite high and scattered, and by the low genetic variability in the meadow, with a very high similarity of specimen at a local scale. Hence, these two putative approaches clearly identify the endangered conservation status of the meadow. They link plant biodiversity and ecophysiology to ecosystem 'health'. Furthermore, they are repeatable and standardizable and could be usefully introduced in meadows monitoring to check environmental quality.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Rotini, A. , Micheli, C. , Valiante, L. and Migliore, L. (2011) Assessment of Posidonia Oceanica (L.) Delile conservation status by standard and putative approaches: the case study of Santa Marinella meadow (Italy, W Mediterranean). Open Journal of Ecology, 1, 48-56. doi: 10.4236/oje.2011.12006.

References

[1] Gobert, S., Cambridge, M.L., Velimirov, B., Pergent, G., Lepoint, G., Bouquegneau, J.-M, Pergent-Martini, C. and Walker, D.I. (2006) Biology of Posidonia, Biology, Ecology and Conservation, Springer, the Netherlands, 387-408.
[2] Short, F.T., Polidoro, B., Livingstone, S.R., Carpenter, K.E., Bandeira, S., Bujang, J.S., Calumpong, H.P., Car- ruthers, T.J.B., Coles, R.G., Dennison, W.C., Erftemeijer, P.L.A., Fortes, M.D., Freeman, A.S., Jagtap, T.G., Kamal, A.H., Kendrick, G.A., Judson Kenworthy, W., La Nafie, Y.A., Nasution, I.M., Orth, R.J., Prathep, A., Sanciangco, J.C., Tussenbroek, B.V., Vergara, S.G., Waycott, M. and Zieman, J.C. (2011) Extinction risk assessment of the world's seagrass species, Biological Conservation, 144, 196
[3] Orth, R., Carruthers, T., Dennison, W., Duarte, C., Fourq- urean, J., Heck, K.L., Hughes, A.R., Kendrick, G.A., Kenworthy, W.J., Olyarnik, S., Short, F.T., Waycott, M. and Williams, S.L. (2006) A global crisis for seagrass ecosystems, Bioscience, 56, 987-996. doi.10.1641/0006-3568(2006)56[987:AGCFSE]2.0.CO;2
[4] Boudouresque, C.F., Bernard, G., Bonhomme, P., Charb-onnel, E., Diviacco, G., and Meinesz, A., Pergent G., Pergent-Martini C., Ruitton S., Tunesi L.. (2006) Préservation et conservation des herbiers à Posidonia oceanica, RaMoGe publication, Monaco.
[5] Pergent, G., Pergent-Martini, C. and Boudouresque, C. F. (1995) Utilisation de l’herbier à Posidonia oceanica comme indicateur biologique de la qualité du milieu littoral en Méditerranée: état de connaissances, Mésogée, 54, 3-27.
[6] Romero, J., Martinez-Crego, B., Alcoverro, T. and Perez, M. (2007) A multivariate index based on the seagrassPosidonia oceanica (POMI) to assess ecological status of coastal waters under the Water Framework Directive (WFD), Marine Pollution Bulletin, 55, 196-204. doi.10.1016/j.marpolbul.2006.08.032
[7] Short, F.T. and Wyllie-Echeverria, S. (1996) Natural and human-induced disturbances of seagrasses, Enviromental Conservation, 23, 17-27. doi.10.1017/S0376892900038212
[8] Gobert, S., Sartoretto, S., Rico-Raimondino, V., Andral, B., Chery, A., Lejeune, P. and Boissery, P. (2009) Assessment of the ecological status of Mediterranean French coastal waters as required by the Water Fra- mework Directive using the Posidonia oceanica Rapid Easy Index: PREI, Marine Pollution Bulletin, 58, 17-27-1733. doi.10.1016/j.marpolbul.2009.06.012
[9] Lopez y Royo, C., Casazza, G., Pergent-Martini, C. and Pergent, G. (2010) A biotic index using the seagrass Posidonia oceanica (BiPo), to evaluate ecological status of coastal waters, Ecological Indicators, 10, 380-389. doi.10.1016/j.ecolind.2009.07.005
[10] Pergent-Martini, C., Leonia, V., Pasqualini, et al. (2005) Descriptors of Posidonia oceanica meadows: Use and application, Ecological Indicators, 5, 213-230. doi.10.1016/j.ecolind.2005.02.004
[11] Montefalcone, M. (2009) Ecosystem health assessment using the Mediterranean seagrass Posidonia oceanica: a review, Ecolological Indicators, 9, 595-604. doi.10.1016/j.ecolind.2008.09.013
[12] Martínez Crego, B., Alcoverro, T. and Romero, J. (2008) Biotic indices for assessing the status of coastal waters: a review of strengths and weaknesses, Journal of Env- ironmental Monitoring, 12, 1013-1028.
[13] Micheli, C., Paganin, P., Peirano, A., Caye, G., Meinesz, A. and Bianchi, C.N. (2005) Genetic variability of Posidonia oceanica (L.) Delile in relation to local factors and bioge- ographic patterns, Aquatic Botany, 82, 210-221. doi.10.1016/j.aquabot.2005.03.002
[14] Micheli, C., Spinosa, F., Aliani, S., Gasparini, G.P., Molcard, A. and Peirano, A. (2010) Genetic input by Posidonia oceanica (L.) Delile fruits dispersed by cur-rents, Plant Biosystems, 144, 333-339. doi.org/10.1080/11263501003764798
[15] Migliore, L., Rotini, A., Randazzo, D., Albanese, N.N. and Giallongo, A. (2007) Phenols content and 2-Delectrophoresis protein pattern: a promising tool to monitor Posidonia meadows health state, BMC Ecology, 7, 6.
[16] Dixon, R.A. and Paiva, N.L. (1995) Stress-induced phen- ylpropanoid metabolism, The Plant Cell, 7, 10-85-1097.
[17] Bate-Smith, E.C. (1968) The phenolic constituents of plants and their taxonomy significance, II Monocotyledons, Journal of the Linnean Society of London: Botany, 60, 325-356. doi.10.1111/j.1095-8339.1968.tb00094.x
[18] Mc Clure, J.W. (1970) Secondary constituents of aquatic angiosperms. In Harborne, J.B., Ed., Phytochemical Phylogeny, Academic Press, London, 233-268.
[19] Zapata, O. and Mc Millan, C. (1979) Phenolic acids in seagrasses, Aquatic Botany, 7, 307-317. doi.10.1016/0304-3770(79)90032-9
[20] Cuny, P., Serve, L., Jupin, H. and Boudouresque, C.F. (1995) Water soluble phenolic compounds of the marine phanerogam Posidonia oceanica in a Mediterranean area colonised by the introduced chlorophyte, Caulerpa taxifolia, Aquatic Botany, 52, 237-242. doi.10.1016/0304-3770(95)00504-8
[21] Dumay, O., Costa, J., Desjobert, J.M. and Pergent, G. (2004) Variations in the concentration of phenolic compounds in the seagrass Posidonia oceanica under conditions of competition, Phytochemistry, 65, 3211-3220. doi.10.1016/j.phytochem.2004.09.003
[22] Ferrat, L., Pergent-Martini, C., Romeo, M. and Pergent, G. (2003) Hydrosoluble phenolic compounds production in a Mediterranean seagrass according to mercury contamina- tion, Gulf of Mexico Science Journal, 21, 108.
[23] Cannac, M., Ferrat, L., Pergent-Martini, C., Pergent, G. and Pasqualini, V. (2006) Effects of fish farming on flavonoids in Posidonia oceanica, Science of the Total Environment, 370, 91-98. doi.10.1016/j.scitotenv.2006.07.016
[24] Fresi, E., Dolce, T., Forni, C., Lorenzi, C., Migliore, L., Rizzelli, D. and Scardi, M. (2004) La prateria di Posidonia oceanica (L.) Delile di Talamone (Grosseto, Italia): struttura e stato di salute, Conference: Le scienze naturali, economiche e giuridiche nello studio e per la gestione degli ambienti acquatici, 18-22 October 2004; Terrasini, Palermo, CONISMA-AIOL.
[25] Procaccini, G., Ruggiero, M.V. and Orsini, L. (2002) Genetic structure and distribution of microsatellite population genetic diversity in Posidonia oceanica in the Mediterranean basin, Bullettin of Marine Science, 71, 1291-1297.
[26] Ruggiero, M.V., Turk, R. and Procaccini, G. (2002) Genetic identity and homozygosity in North-Adriatic populations of Posidonia oceanica: an ancient, postglacial clone? Conservation Genetics, 3, 71-74. doi.10.1023/A:1014207122382
[27] Serra, I.A., Innocenti, A.M., Di Maida, G., Calvo, S., Migliaccio, M. and Zambianchi, E., et al. (2010) Genetic structure in the Mediterranean seagrass Posidonia oceanica: disentangling past vicariance events from con- temporary patterns of gene flow, Molecular Ecology, 19, 557-568. doi.org/10.1111/j.1365-294X.2009.04462.x
[28] Arnaud-Haond, S., Marbà, N., Diaz-Almela, E., Serr?o, E.A. and Duarte, C.M. (2010) Comparative analysis of stability-genetic diversity in seagrass (Posidonia oceanica) meadows yields unexpected results, Estuaries and Coasts, 33, 878-889. doi.10.1007/s12237-009-9238-9
[29] Procaccini, G., Olsen, J.L. and Reusch, T.B.H. (2007) Contribution of genetics and genomics to seagrass biology and conservation, Journal of Experimental Marine Biology and Ecology, 350, 234-259. doi.10.1016/j.jembe.2007.05.035
[30] Dong, Y.H., Gituru, R.W. and Wang, Q.F. (2010) Genetic variation and gene flow in the endagered aquatic fern Ceratopteris pteridoides in China and conservation implications, Annales Botanici Fennici, 47, 34-44.
[31] Jones, T.C., Gemmill, C.E.C. and Pilditch, C.A. (2008) Genetic variability of New Zealand seagrass (Zosteramuelleri) assessed at multiple spatial scales, Aquatic Botany, 88, 39-46. doi.10.1016/j.aquabot.2007.08.017
[32] Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic ma-rkers, Nucleic Acids Research, 18, 6531-6535. doi.10.1093/nar/18.22.6531
[33] Waycott, M. (1998) Genetic variation, its assessment and implications to the conservation of seagrasses, MolecularEcology, 7, 793-800. doi.10.1046/j.1365-294x.1998.00375.x
[34] Alberto, F., Mata, L. and Santos, R. (2001) Genetic ho- mogeneity in the seagrass Cymodocea nodosa at its northern Atlantic limit revealed through RAPD, Marine Ecology Progress Series, 221, 299-301. doi.10.3354/meps221299
[35] Procaccini, G., and Mazzella, L. (1996) Genetic variabil- ity and reproduction in two Mediterranean seagrasses. In: Kuo, J., Phillips, R.C., Walker, D.I., Kirkman, H., Eds., Seagrass Biology: Proceedings of an international wor- kshop, SCIENCES UWA, Rottnest Island, Western Aus- tralia, 85-92.
[36] Micheli, C., Paganin, P., Maffucci, M., Nascetti, G., Rismondo, A. and Curiel, D. (2004) Zostera marina in Venice lagoon: a genetic study, Rapports et Proces-Verbaux des Réunions Commission International pour l'Exploration Scientifique de la Mer Méditerranée, 37,536.
[37] Diviacco, G., Spada, E. and Virno Lamberti, C. (2001) Le Fanerogame marine del Lazio, Descrizione e Cartografia delle prateria di Posidonia oceanica e dei prati di Cym- odocea nodosa, Roma: ICRAM.
[38] ARPALazio. (2007) Quarto rapporto sulla qualità delle acque superficiali e sotterranee della Provincia di Roma,http://www.arpalazio.it/dati2007/IV_rapportoAcqua.pdf. Accessed 08/23/2011.
[39] Dante, G. (2010) Development of sustainable strategies for conservation and management of Posidonia oceanica,(Linneo) Delile 1813, meadow: a case study within a site of community importance, Ph.D. dissertation, University of Tuscia, Viterbo (Italy). http://dspace.unitus.it/bistream/2067/996/1/gdante_tesid.pdf. Accessed 08/23/2011
[40] Rotini, A., Anello, L., Di Bernardo, M., Giallongo, A. and Migliore, L. (2011) A monitoring study of a Posidonia oceanica meadow coupling total phenol content and 2-D electrophoresis pattern of proteins, Glycine max, submitted.
[41] Giraud, G. (1977) Essai de classement des herbiers dePosidonia oceanica (L.) Delile, Botanica Marina, 20, 487-491. doi.10.1515/botm.1977.20.8.487
[42] Pergent, G. (1990) Lepidochronological analysis of the seagrass Posidonia oceanica (L.) Delile: a standardizedapproach, Aquatic Botany, 37, 39-54. doi.10.1016/0304-3770(90)90063-Q
[43] Legrand, B. (1977) Action de la lumière sur les peroxydases et sur les teneurs en composés phénoliques de tissus de feuilles de Cichorium intybus L. cultivés in vitro, Biologia Plantarum, 19, 27-33.
[44] Booker, F.L. and Miller, J.E. (1998) Phenylpropanoid metabolim and phenolic composition of soybean (Glycine max L.) leaves following exposure to ozone, Journal of Experimental Botany, 49, 1191-1202. doi.10.1093/jexbot/49.324.1191
[45] Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983) A plant DNA mini preparation: version II, Plant Molecular Biology Reporter, 1, 19-21. doi.10.1007/BF02712670
[46] Buia, M.C., Gambi, M.C. and Dappiano, M. (2004) The seagrass ecosystems. In: Gambi, M.C., Dappiano, M., Eds., Mediterranean Marine Benthos: a manual for its sampling and study, Biologia Marina Mediterranea, 11, 133-183.
[47] Pergent, G., Pergent-Martini, C. and Boudouresque, C.F. (1995) Utilisation de l’herbier à Posidonia oceanica comme indicateur biologique de la qualité du milieu littoral en Méditerranée: état de connaissances, Mésogée,54, 3-27.
[48] Drew, E.A. (1971) Underwater Science. An introduction to experiments by divers, In: Woods, J.D., Lithgoe, J.N., Eds., Botany, Academic Press, London, 175-233.
[49] Borg, J.A. and Schembri, P.J. (1995) Preliminary data on bathymetric and temporal changes in the morphology of a maltese Posidonia oceanica (L.) Delile meadow, Rapports et Proces-Verbaux des Réunions Commission International pour l'Exploration Scientifique de la Mer Méditerranée, 34, 20.
[50] Gobert, S., Kyramarios, M., Lepoint, G., Pergent-Martini, C. and Bouquegneau J.M. (2003) Variation à différentséchelles spatiales de l’harbier à Posidonia oceanica (L.) Delile; effects sur les paramètres physico-chimiques du sediment, Oceanologica Acta, 26, 199-207. doi.10.1016/S0399-1784(02)00009-9
[51] Pergent, G. and Pergent-Martini, C. (1988) Phenologie de Posidonia oceanica (Linnaeus) Delile dans le bassin méditerranéen, Annales de l’Institut Océanographique Paris, 64, 79-100.
[52] Diviacco, G. and Coppo, S. (2007) Atlante degli habitat marini della Liguria: descrizione e cartografia delle praterie di Posidonia oceanica e dei principali popo-lamenti marini costieri, Edizioni Grafiche Amadeo, Imperia.
[53] Peirano, A. and Bianchi, C.N. (1997) Decline of the seagrass Posidonia oceanica in response to environmental disturbance: a simulation-like approach off Liguria (NW Mediterranean Sea), The Response of marine organisms to their environments, University of Southampton, U.K, 87-95.
[54] Adams, S.M. (2003) Establishing causality between environmental stressors and effects on aquatic ecosystems, Human and Ecological Risk Assessment, 9, 17-35. doi.10.1080/713609850
[55] Chapman, P.M., McDonald, B.G., and Lawrence, G. S. (2002) Weight-of-evidence issues and frameworks for sediment quality (and other) assessments, Human and Ecological Risk Assessment, 8, 1489-515. doi.10.1080/20028091057457
[56] Sanz-Lázaro, C., and Marin, A. (2009) A manipulative field experiment to evaluate an integrative methodology for assessing sediment pollution in estuarine ecosystems, Science of the Total Environment, 407, 3510-35

  
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