Effect of enpishin (press tack acupuncture needles) on autonomic function, WBC count and oxidative stress

Mitsunori Tsumaki, Yoshitomo Saita, Hiroshi Ikeda, Kazuo Kaneko, Takehiko Yukishita, Keiko Lee, Sungdo Kim, Shinichi Yokota, Nobuhiro Suetake, Hiroyuki Kobayashi

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DOI: 10.4236/health.2011.37072                

Abstract

Acupuncture as a vital component of traditional medical systems of the Far East has been used to restore and maintain health for over three millennia. However, the neurobiological correlates of this therapy remain largely unknown. The purpose of this study was therefore to determine the effect of enpishin acupuncture (press tack needles (PTN)) on autonomic function, WBC count, and oxidative stress between subjects that received either verum or placebo PTN treatment. Twenty men (mean(SD) age: 36.7(5.1) years) who provided oral consent were randomized to receive either verum PTN (n = 9) or placebo PTN (n = 11). Bi-Digital O-Ring Test (BDORT) was used to select the acupoints for each participant. Autonomic function and oxidative stress level were analyzed before and after the treatment via heart rate variability (HRV) and free radical analysis system (FRAS4), respectively. Pre- and post-treatment WBC count was also evaluated. Verum PTN group had a statistically significant increase (p = 0.008) in Ln TP, an index of overall autonomic activity. Subjects in this group also had a significantly greater (p = 0.006) variation in Ln TP than the placebo PTN group in response to the treatment. Similarly, Ln HF of subjects receiving verum PTN showed a marked increase (p = 0.0026) after the treatment. Moreover, a significantly greater (p < 0.001) variation in mean Ln HF before and after the treatment was noted in the verum group than the placebo group, reflecting a greater parasympathetic activation in the former. In contrast, sympathetic activity was not significantly influenced by verum PTN based on the lack of observed changes in LF/HF ratio. In addition, both groups failed to demonstrate significant variations in pre- and post-treatment mean Ln LF/HF ratio. Further, no significant intergroup difference was found with regard to mean variation in Ln LF/HF ratio. Verum PTN did not induce significant changes in WBC count and markers of oxidative stress, namely reactive oxygen species (ROM) and biological antioxidant potential (BAP). However, a comparison of variations in pre- and post-treatment mean WBC count showed a significant difference (p = 0.020) between the two treatment modes: verum group showed a slight increase (225 ± 384.5), while placebo group showed a minimal decrease (–190 ± 272.6). Our findings suggest that PTN increases overall autonomic activity, particularly the parasympathetic function.

Keywords

Heart Rate Variability; Press Tack Needles; Acupoints; Bi-Digital O-Ring Test; Oxidative Stress

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Kaptchuk, T.J. (2002) Acupuncture: Theory, efficacy, and practice. Annals of Internal Medicine, 136, 374-383.
[2]	Ernst, E. and White, A. (2000) The BBC survey of complementary medicine use in the UK. Journal of Alternative Complementary Medicine, 8, 32-36.
[3]	Tindle, H.A., et al. (2005) Trends in use of complementary and alternative medicine by US adults: 1997-2002. Alternative Therapies in Health and Medicine, 11, 42-49.
[4]	Deadman, P., et al. (2007) A manual of acupuncture. Sung In Printing America, Inc., Daly City.
[5]	Reaves, W. (2008) Acupuncture and the athlete. http://www.acsm.org
[6]	Pelham, T.W., et al. (2001) Acupuncture in human performance. Journal of Strength and Conditioning Research, 15, 266-271.
[7]	Miyamoto, T., et al. (2008) Symposium research on acupuncture and moxibustion in the sports field. Journal of the Japan Society of Acupuncture and Moxibustion, 58, 166-178.
[8]	Anderson, S. (1997) Physiological mechanisms in acupuncture, In: Hopwood, V., et al. Eds., Acupuncture and Related Techniques in Physical Therapy, Churchill Livingstone, New York, 19-39.
[9]	Sato, A., et al. (2000) Calcitonin gene-related peptide produces skeletal muscle vasodilation following antidromic stimulation of unmyelinated afferents in the dorsal root in rats, Neuroscience Letters, 283, 137-140.
[10]	National Institutes of Health (1998) NIH Consensus Con- ference Acupuncture. Journal of the American Medical Association, 280, 1518-1524.
[11]	Litscher, G. (2010) Translational research in acupuncture-teleacupuncture bridges science and practice. Health, 2, 16-19. doi:10.4236/health.2010.21003
[12]	White, A., Hayhoe, S., et al., (2001) Survey of adverse events following acupuncture (SAFA): A prospective study of 32,000 consultations. Acupuncture in Medicine, 19, 84-92. doi:10.1136/aim.19.2.84
[13]	Miyamoto, T., et al. (2003) Effect of acupuncture using press needle on muscle pain and stiffness after marathon race-Double blind randomized control trial. Japanese Journal of Oriental Medicine, 54, 939-944.
[14]	Sawazaki, K., et al. (2008) Acupuncture can reduce perceived pain, mood disturbances and medical expenses related to low back pain among factory employees. Industrial Health, 46, 336-340. doi:10.2486/indhealth.46.336
[15]	Kaneko, Y., et al. (2006) The effect of press tack needle treatment on muscle soreness after triathlon race-placebo controlled study. Journal of Japan Society of Acupunct and Moxibustion, 56, 158-165.
[16]	Omura, Y. (1985) A new, simple, non-invasive imaging technique of internal organs and various cancer tissues using extended principles of the “Bi-Digital O-Ring test” without using expensive imaging instruments or exposing the patient to any undesirable radiation part 1. Acupuncture and Electro-Therapeutics Research, 10, 255- 277.
[17]	Omura, Y. (1998) Practice of Bi-Digital O-Ring test. Ido-No-Nihonsha, Yokosuka.
[18]	World Health Organization (2009) WHO standard acupuncture point locations in the western pacific region 2008. Ido-No-Nippon-Sha, Yokosuka.
[19]	Martin, G.J., et al. (1987) Heart rate variability and sudden death secondary to coronary artery disease during ambulatory electrocardiographic monitoring. American Journal of Cardiology, 60, 86-89. doi:10.1016/0002-9149(87)90990-8
[20]	Matsumoto, T., et al. (2008) Altered autonomic nervous system activity as a potential etiological factor of premenstrual syndrome and premenstrual dysphoric disorder. Psychosomatic Medicine, 48, 1011-1024.
[21]	Kuo, T.B., et al. (1999) Effect of aging on gender differences in neural control of heart rate. American Journal of Physiology, 277, 2233-2239.
[22]	Nishijo, K., Mori, H., et al. (1995) Scientific approach for acupuncture. Jounal of Japan Society of Acupunct and Moxibustion, 45, 177-191.
[23]	Kawakita, K., et al. (2008) Do Japanese style acupuncture and moxibustion reduce symptoms of the common cold? Electronic Centralised Aircraft Monitor, 5, 481- 489.
[24]	Chen, L. (2006) Comparison of therapeutic effects between normal acupuncture and shallow needling with short-needle on periarthritis of shoulder. Zhongguo Zhen Jiu, 26, 647-648.
[25]	Vickers, A.J., et al. (2006) Acupuncture is a feasible treatment for post-thoracotomy pain: Results of a prospective trial. http://www.biomedcentral.com/1471-2253/6/5
[26]	Kotani, N., et al. (2001) Preoperative intradermal acupuncture reduces postoperative pain, nausea and vomiting, analgesic requirement, and sympathoadrenal responses. Anesthesiology, 95, 349-356. doi:10.1097/00000542-200108000-00015
[27]	Wu, Z.H. (2009) Effects of the multiple needling with shallow insertion for simple obesity: A clinical observation on lipid metabolism and on the chest, waist and hip circumferences. Journal of Traditional Chinese Medicine, 29, 179-181. doi:10.1016/S0254-6272(09)60060-1
[28]	Sato, T., et al. (1996) Acupuncture stimulation enhances splenic natural killer cell cytotoxicity in rats. Japanese Journal of Physiology, 46, 131-136. doi:10.2170/jjphysiol.46.131
[29]	Kushioka, S., et al. (2002) Assessment of acupuncture upon physiological system-qualitative and quantitative assessment on leukocyte and lymphoid cell subsets after acupuncture. Jounal of Japan Society of Acupunct and Moxibustion, 52, 17-19.