Heat-Resistant Properties of α-Chymotrypsin Adsorbed onto Biomass Charcoal Powder

Abstract

Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was shifted to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the kind of BCP.

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Noritomi, H. , Kurihara, S. , Endo, N. and Kato, S. (2014) Heat-Resistant Properties of α-Chymotrypsin Adsorbed onto Biomass Charcoal Powder. Journal of Biomaterials and Nanobiotechnology, 5, 179-185. doi: 10.4236/jbnb.2014.53021.

Conflicts of Interest

The authors declare no conflicts of interest.

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