Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids ()
Masahiro Sato,
Emi Inada,
Issei Saitoh,
Yuko Matsumoto
Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka, Kagoshima, Japan.
Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Gakkocho-dori, Chuo-ku, Niigata, Japan.
Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Sakuragaoka, Kagoshima, Japan.
DOI: 10.4236/jbise.2014.73013
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Abstract
Minipreparation (MiniPrep) analysis is an essential step
for obtaining a recombinant plasmid that carries a DNA insert containing a gene
of interest. The most commonly used method for this involves cultivation of
transformed Escherichia coli (E. coli) in liquid medium, brief centrifugation
for precipitation of bacterial pellets, and
subsequent lysis of the pellets. This process is time-consuming and
laborious, especially when the sample number is high. Here, we describe a more
convenient method for MiniPrep analysis that utilizes solid medium-based
cultivation of bacteria.
Share and Cite:
Sato, M. , Inada, E. , Saitoh, I. and Matsumoto, Y. (2014) Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids.
Journal of Biomedical Science and Engineering,
7, 105-107. doi:
10.4236/jbise.2014.73013.
Conflicts of Interest
The authors declare no conflicts of interest.
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