Evaluation of antibiotic activity produced by Pseudomonas aeruginosa LV strain against Xanthomonas arboricola pv. pruni

Fernanda Corrêa da Silva Vasconcellos, Admilton Gonçalves de Oliveira, Lucilene Lopes-Santos, amile Priscila de Oliveira Beranger, Martha Viviana Torres Cely, Ane Stefano Simionato, Juliana Fonteque Pistori, Flavia Regina Spago, João Carlos Palazzo de Mello, Juca Abramo Barrera San Martin, Celia Guadalupe Tardeli de Jesus Andrade, Galdino Andrade

Departamento de Biologia Geral, Laboratório de Microscopia e Microanálise, Universidade Estadual de Londrina, Londrina, Brazil.
Departamento de Farmácia e Farmacologia, Laboratório de Produtos Fitoterápicos, Universidade Estadual de Maringá, Maringá, Brazil.
Departamento de Microbiologia, Laboratório de Ecologia Microbiana, Universidade Estadual de Londrina, Londrina, Brazil.

DOI: 10.4236/as.2014.51008                

Abstract

Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is considered as a major problem in peach orchards. Copper and antibiotics are used to control, and biocontrol should be a new alternative with low environment impact. The objective was evaluated by the antibiotic activity of the metabolite produced by Pseudomonas aeruginosa LV strain. The free cells supernatant was fractionated with a serial organic solvent with crescent polarity and a dichloromethane phase was concentrated and lyophilised, and after was fractionated using vacuum liquid chromatography. The antibiotic activity of the F3 fraction containing an organometallic compound was tested on Xap in vitro and in a greenhouse conditions. Plants were sprayed with F3 before or after Xap infection and the results showed changes in exopolysaccharides and cell morphology. The F3 concentration of 450 μg·mL^-1 was more effective. The results showed that F3 fraction could be a new alternative to control bacterial spot.

Keywords

Peach Tree; Bacterial Spot; Biological Control; Leaf Lesions; Xanthomonas arboricola Pv. pruni

Conflicts of Interest

The authors declare no conflicts of interest.

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