Share This Article:

Comparative study of the effect of Bacillus thuringiensis on larval populations of Culex pipiens L. (Diptera-Culicidae) of the City of Tlemcen (Algeria)

Full-Text HTML Download Download as PDF (Size:1365KB) PP. 265-271
DOI: 10.4236/oje.2013.33030    2,650 Downloads   4,587 Views   Citations


In the cities of Algeria, Culex pipiens L. (Diptera: Culicidae) is the mosquito which presents most interest because of its wide geographical distribution and of its abundance which engender a strong nuisance. Besides, its role of vector of the virus West Nile arouses a particular interest in the Mediterranean Basin. These insects are generally controlled by conventional insecticides for the greater part, chemicals which cause in the long term side effects (effects on the not aimed bodies and the resistance of the aimed species). A research for the effect of the bioinsecticide Bacillus thuringiensis (granulated commercial shape in 200 IUT1/mg) was realized on préimaginales populations of the artificial deposits sites (taken directly of them natural the deposits sites) and cleansed populations (stemming from a breeding) having never been handled previously, taking into account local weather and physico-chemical conditions. Analyses of variance, allowed to determine the combined effect of the factor measure and of the factor time which by increasing, increase the efficiency of the product. The results of the rates of mortalities registered after treatment allowed to loosen the DL50 and the DL90 for every embryonic stage. From the results, we estimated the degree of sensibility of the larva populations of Culex pipiens which have proved heterogeneous in partial tolerance in Bacillus thuringiensis for the populations of the sensitive artificial and homogeneous the deposits sites for those stemming from the breeding.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Tabti, N. and Abdellaoui-Hassaïne, K. (2013) Comparative study of the effect of Bacillus thuringiensis on larval populations of Culex pipiens L. (Diptera-Culicidae) of the City of Tlemcen (Algeria). Open Journal of Ecology, 3, 265-271. doi: 10.4236/oje.2013.33030.


[1] Hassaine, K. (2002) Les Culicides (Diptera—Nemato cera) de l’Afrique méditerranéenne. Bioécologie d’Aedes caspius et d’Aedes detritus des marais salés, d’Aedes maiae des rock-pools littoraux et de Culex pipiens des zones urbaines de la région occidentale algérienne. Thèse Doc. D’état. Univ. Tlemcen, 203.
[2] Gilot, B. (1969) Répartition altitudinale des moustiques (Diptera, Culicidae). Exemple du Dauphiné du Nord (Alpes francaises) Cah. O.R.S.T.O.M. sér. Ent. Méd. Parasitol., VII, 226-227.
[3] Bendjaber, M. (1985) Contribution à l’étude de la faune culicidienne dendrolimnique de la subéraie en pays Zaer (Maroc) (Aspect systématique). Thèse D.E.A. Uni. Paul Sabatier Toulouse IV, 111.
[4] Metge, G. and Belakoul, N. (1989) Colonisation d’un nouvel habitat par Culex pipiens (Diptera, Culicidae): Le creux d’arbre des subéraies en pays Zaer. Maroc, Ann. Limn, 25, 73-80. doi:10.1051/limn/1989009
[5] El Hassar-Benkalfat, C. (1991) Cartographie de Culex pipiens (Diptera, Culicidae) en milieu urbain (ville de Tlemcen); recherche des causalités de la dynamique démographique des stades préimaginaux. Thèse Mag. University, Tlemcen, 143.
[6] Berchi, S. (2000) Bioécologie de Culex pipiens L. (Dip tera: Culicidae) dans la région de Constantine et perspectives de lutte. Thèse Doc. Es. Scien. Univ. Constantine, 133.
[7] Georghiou, G.P. and Lagunes-Tejeda, A. (1991) The occurrence of resistance to pesticides in arthropods. An index of cases reported through to Baygon. Bull. W.H.O., 35, 691-708.
[8] Glare, T.R. and O’Gallaghan, M. (1998) Environmental and health impacts of Bacillus thuringiensis israelensis. Report for the Ministry of Health, 58.
[9] Swaroop, S. and Uemera, K. (1966) Probit analysis. World Health Organisation, Geneva.
[10] Hervy, J.P. and Coosepians, W. (1979) L'élevage des Aedes et des Anophèles. Réalisation et intérêt pratique. IXe Conférence technique. Bobo-Dioulasso du 5 au 8 juin, 668-681.
[11] Abott, A. (1925) A Method for computing effectiveness an insecticide. Journal of Economic Entomology, 18, 122-254.
[12] Sinègre, G., Jullien, J.L. and Crespo, O. (1976) Résistance de certaines populations de Culex pipiens L. au chloropirifos (Dursban) en Languedoc-Roussillon. Cahier de O.R.S.T.O.M. Ser. Ent. Méd. Parasito., 14, 49-59.
[13] Aly, C., Mulla, M.S., Xu, B.Z. and Schnetter, W. (1988) Rate of ingestion by mosquito larvae (Diptera: Culicidae) as a factor of the effectiveness of a bacterial stomach toxin. Journal of Medical Entomology, 25, 191-196.
[14] Aly, C. and Mulla, M.S. (1986) Orientation and ingestion rates of larval Anopheles albimanus in response to floating particles. Entomologia Experimentalis et Applicata, 42, 83-90. doi:10.1111/j.1570-7458.1986.tb02191.x
[15] Mulla, M.S. (1990) Activity, field efficacy, and use of Bacillus thuringiensis israelensis against mosquito, 134-160.
[16] Rashed, S.S. and Mulla, M.S. (1989) Factors influencing ingestion of particulate materials by mosquito larvae (Diptera: Culicidae). Journal of Medical Entomology, 26, 210-216.
[17] Becker, N. and Margalit J. (1993) Use of Bacillus thuringiensis israelensis against mosquitoes and blackflies, 147-170.
[18] Nayar, J.K., Knight, J.W., Ali, A., Carlson, D.B. and O’Bryan, P.D. (1999) Laboratory Evaluation of biotic and abiotic factors that may influence larvicidal activity of Bacillus thuringiensis serovar. Israelensis against two Florida mosquito species. Journal of the American Mosquito Control Association, 15, 32-42.
[19] Boisvert, M. and Boisvert, J. (2000) Effects of Bacillusthuringiensis var. israelensis on target and nontarget organisms: a review of laboratory and field experiments. Biocontrol Science and Technology, 10, 517-561. doi:10.1080/095831500750016361
[20] Ramoska, W.A., Watts S. and Rodrigues, R.E. (1982) Influence of suspended particles on the activity of Bacillus thuringiensis ser. H-14 against mosquito larvae. Journal of Economic Entomology, 75, 1-4.
[21] Margalit, J. and Bobroglio, H. (1984) The effect of organic materials and solids in water on the persistance of Bacillus thuringiensis var. israelensis. Zeitschrift fuer Angewandte Entomologie, 97, 516-520. doi:10.1111/j.1439-0418.1984.tb03785.x
[22] Purcell, B.H. (1981) Effects of Bacillus thuringiensis var. Israelensis on Aedes taeniorhynchus and some non-target organisms in the salt marsh. Mosquito News, 41, 476-484.
[23] Sinègre, G., Gaven, B. and Vigo, G. (1981) Contributionà la normalisation des épreuves de laboratoire concernant des formulations expérimentales et comer-ciales du sérotype H-14 de Bacillus thuringiensis. II. Influence de la température, du chlore résiduel, du pH et de la profondeur de l’eau sur l’activité biologique d’une poudre primaire. Cahiers O.R.S.T.O.M. Série En-tomologie Médicale et Parasitologie, 19, 149-155.
[24] Car, M. and De Moor, F.C. (1984) The responce of Vaal River drift and benthos to Simulium (Diptera: Nematocera) control using Bacillus thuringiensis var. israelensis (H-14). Onderstepoort Journal of Veterinary Research, 51, 155-160.

comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.