The Fusability of Erythrocytes as a Method for Evaluating the Instability of Their Membranes


A novel approach to the study of erythrocytes instability based on assessment fusability of their plasma membrane is presented. The fusability of erythrocytes normal blood, stored for 7 days at 4°C, and type 2 patients with diabetes were studied. Human erythrocytes were fused by incubation with La3+ at final concentrations from 50 to 200 μM. Erythrocytes aggregates were incubated at 37°C for 120 min. The fusability of erythrocytes was evaluated using a light microscope. It was shown that La3+ induced extensive fusion of erythrocytes after 7 days of blood storage and erythrocytes of patients with diabetes. Incubation of normal erythrocytes at 37°C for 120 min does not induce cell fusion.

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Y. Sheremet’ev, A. Popovicheva, M. Rogozin and G. Levin, "The Fusability of Erythrocytes as a Method for Evaluating the Instability of Their Membranes," Open Journal of Biophysics, Vol. 3 No. 3, 2013, pp. 198-200. doi: 10.4236/ojbiphy.2013.33023.

Conflicts of Interest

The authors declare no conflicts of interest.


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