Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD

Abstract

A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).

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Wang, S. , Wang, M. and Wang, Q. (2013) Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD. American Journal of Molecular Biology, 3, 108-114. doi: 10.4236/ajmb.2013.32015.

Conflicts of Interest

The authors declare no conflicts of interest.

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