Cell Cycle Arrest Mediates Global DNA Methylation Patterns in Normal Human Keratinocytes, Epidermoid Carcinoma Cells and Murine Embryonic Fibroblasts

John J. Wille, Jong Y. Park

Bioderm Technologies Inc, Trenton, USA.
Division of Cancer Prevention and Control, Moffitt Cancer Center, Tampa, USA.

DOI: 10.4236/jct.2013.41030                

Abstract

The 5-methylationcytosine (5-MC) DNA content of murine embryonic fibroblasts arrested in G₁ by four growth conditions (Gc, Gn, Gd, and Gs) were hypermethylated relative to rapidly growing (RG) fibroblasts. Normal human keratinocytes (NHK) arrested in G₁ by suspension were hypermethylated relative to RG cultures. Four RG cultures of epidermoid carcinoma cells (ECC) were hypomethylated relative to RG NHK cultures, and two cultures (SCC25 and A431) were further hypomethylated by SUS-induced arrest. Linear regression analyses established a positive linear correlation between growth rate and 5-MC content for three murine fibroblasts lines, and a negative correlation for both NHK and ECC lines.

Keywords

Anoikis; Epitheliod Carcinoma Cells Cell Cycle; DNA Methylation; G1 Phase; Restriction Points

Conflicts of Interest

The authors declare no conflicts of interest.

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