Effects of Baicalin and Ligustrazine on airway inflammation and remodeling and underlying mechanism in asthmatic rats


Aim: To explore the effects of Baicalin and Ligustrazine on airway inflammation and construction and underlying mechanisms through the expressions of GATA-3, IL-5, MMP-9 and TIMP-1 in asthmatic rats. Methods: 30 Wistar rats were randomly divided equally into five groups. Lung tissues were sliced. WBC and Eos in lung tissue were estimated by HE stain and the expressions of IL-5, GATA-3, MMP-9, TIMP-1 and collagen type IV in lung tissue were observed by immunohistochemistry. The airway wall and airway smooth muscle thicknesses were measured by computed image analysis system. Results: Compared with asthma group, EOS counts and the expression of IL-5 and GATA-3 in the lung tissue were significantly lower in normal controlled groups (P < 0.05 or P < 0.01). Additionally, the thickness of airway wall and airway smooth muscle did significantly increase, and the expressions of collagen type Ⅳ, MMP-9 and TIMP-1 were significantly higher in asthma group (P < 0.05). With the intervention of Baicalin or Ligustrazine, EOS decreased, and the thicknesses of airway wall and airway smooth muscle became thinner compared with asthma group. Meanwhile, the expression of collagen type IV, IL-5, GATA-3, MMP-9 and TIMP-1 significantly decreased (P < 0.05). Airway wall thickness and collagen type Ⅳ were associated with Eos, IL-5, TAGA-3, MMP-9, TIMP-1 and MMP-9/ TIMP-1. Conclusion: Two herbs could diminish infiltration of EOS with inhibiting the expressions of IL-5, and GATA-3, meanwhile, decrease the deposition of collagen type IV and the thickness of the airway smooth muscle through regulating MMP-9, TIMP-1 level and the balance between MMP-9 and TIMP-1, additionally, had synergetic effects.

Share and Cite:

Wu, S. , Wu, H. , Wu, Y. , Liu, L. , Cai, R. and Xu, Y. (2012) Effects of Baicalin and Ligustrazine on airway inflammation and remodeling and underlying mechanism in asthmatic rats. Advances in Bioscience and Biotechnology, 3, 585-591. doi: 10.4236/abb.2012.35076.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Holgate ST, Peters-Goiden M, Jpanettieri RA, et al. Roles of cysteinyl leukotrienes in airway inflammation, smooth muscle function, and remodeling[J]. J Allergy Clin Immunol, 2003, 111(1suppl): S18 – S34.
[2] Menzies-Gow A,Robinson DS. Eosinophils eosinophilis Cytokines (interleukin-5),and antieosinophlllc therapy in asthma. Currpin Med,2002;8:33-38.
[3] Duncan CJA, Lawrie A, Blaylock HG, et al. Reduced eosinophil apoptosis in induced sputum correlates with asthma severity. European Respiratory Journal, 2003; 22: 484-490
[4] Humbles AA,Lloyd CM,Mc Millan SJ,etal.A critical role of eosinophile in allergic airways remodeling. Science, 2004; 305(5691): 1776-1779
[5] Vignola AM, Riccobono L, Mirabella A, et al. Sputum metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis. Am J Respir Crit Care Med, 1998, 158(6): 1945-50
[6] Cataldo DD, Tournoy KG, Vermaelen K, et al. Matrix metalloproteinase-9 deficiency impairs cellular infilatration and bronchial hyperresponsiveness during allergen induced airway inflammation. Am J Pathol, 2002, 161: 491-498..
[7] Jiang B, Zhang S M, Li Q,etal. Protection of Baicalin in asthmatic model of guinea-pigs. PHARMACEUTICAL CARE AND RESEARCH,2001,1(1):36-39.
[8] Ni Q,Dong J C.Experimental Study of the Effect of Components of Three Traditional Chinese Medcines on Airway Allegic Inflammation in Guinea Pigs with Bronchial Asthma. Chinese Journal of Experimental Traditional Medical Formulae,2004,10(4):49-51.
[9] Finotto S,De Sanctis CT,Lehr HA,et al.Treatment of allergic airway inflammation and hyperresponsiveness by antisense-induced local blockade of GATA-3 expression.J Exp Med,2001,193:1247-1260
[10] Palmans E, Kips J C, Paumwels R A. Prolonged allergen exposure induces structural airway changes in sensitized rats[J].Am J Respir Crit Care Med, 2000,161(2 Pt 1):627-635.
[11] Xu S Y,Xu Y J,Zhang Z X,et al.pathological features and mechanism of airway remodeling in asthmatic rats. Acta Med Univ Sci Technol Huazhong 2006,35(4):465–468.
[12] Hoshino M, Takehashi M, Takai Y, et al. Inhaled corticosteroids decrease subepithelial collagen deposition by modulation of the balance between matrix metalloproteinase-9 and inhibitor of metalloproteinase-1 expression in asthma. J Allergy Clin Immunol, 1999, 104(2 Pt 1): 356-363.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.