New Approach for the Determination of Tricyclic Antidepressant Amitriptyline Using β-Cyclodextrin-PEG System via Spectrophotomerty


A new and simple procedure for the spectrophotometric determination of the tricyclic antidepressant drug amitriptyline is proposed. The method is based on enhancement of sensitivity of the [AMIYTP]+ β-cyclodextrin and PEG molecules involved in formation of molecules inclusion complex, in presence of polyethylene glycol (PEG) medium. The mole ratio of [AMIYTP]+ β-cyclodextrin and PEG molecules in inclusion complex were determined by the curve fitting method. The value of molar absorptivity of {[AMIYTP: (β CD)] PEG} complex in term of the drug lies in rage of (2.20 - 2.23) × 104 L·mole–1·cm–1 at absorption maximum 242 nm. The slope, intercept and correlation co-efficient were found to be 14.21, 0.0046, and 0.998, respectively. The effect of analytical variables on the determination of the drug and composition of the ion associated complex are discussed in the paper. This method is applicable in the determina-tion of amitriptyline in the pharmaceutical preparations.

Share and Cite:

P. Soni, S. Sar and R. Patel, "New Approach for the Determination of Tricyclic Antidepressant Amitriptyline Using β-Cyclodextrin-PEG System via Spectrophotomerty," Journal of Analytical Sciences, Methods and Instrumentation, Vol. 2 No. 2, 2012, pp. 103-107. doi: 10.4236/jasmi.2012.22019.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] G. A. Gilman, W. T. Rall, S. A. Nies and P. Taylor, “The Pharmacological Basis of Therapeutics,” Ninth Edition, Mc Graw Hill, New York, 1996.
[2] C. M. Hall and R. A. Nugent, “Encyclopedia of Chemical Technology,” John Wiley & Sons, New York, 1992.
[3] W. C. Bowman and M. L. Rand, “Textbook of Pharmacology,” Blackwell Science Publication, London, 1992.
[4] J. L. Atwood, J. E. D. Daviess, D. D. Mac Nicol and F. Vogtle, “Comprehensive Supramolecular Chemistry Pergamon,” Oxford, 1996.
[5] K. A. Connors, “The Stability of Cyclodextrine Complex in Solution,” Chemical Reviews, Vol. 97, No. 5, 1997, pp. 1325-1357. doi:10.1021/cr960371r
[6] V. T. D’Souza and K. B. Lipkowitz, “Cyclodextrines,” Chemical Reviews, Vol. 98, 1998, p. 1741.
[7] D. O. Thompson, “Cyclodextrine—Enabling Excipients. Their Present and Future Use in Pharmaceuticals,” Critical Reviews in Therapeutic Drug Carrier, Vol. 14, No. 1, 1997, pp. 1-104.
[8] F. A. Mohamed, H. A. Mohamed, S. A. Hussein and S. A. Ahmed, “A Validated Spectrofluorimeter Method for Determination of Some Psychoactive Drug,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 39, No. 1-2, 2005, pp. 139-146. doi:10.1016/j.jpba.2005.03.024
[9] J. E. F. Reynolds (Ed.), “The Extra Pharmacopoeia,” 30th Edition, The Pharmaceutical Press, London, 1993, p. 8140.
[10] J. Karpinska, J. Suszymska and J. Trace, “The Spectrophometric Simultaneous Determination of Amitriptyline and Chloropromazine Hydrochloride in Their Binary Mixture,” Microprobe Technology, Vol. 19, 2001, pp. 355-364. doi:10.1081/TMA-100105051
[11] A. F. Faten, N. El-Dien, G. Mohamed Gehad and A. Mohamed, “Nehad Spectrophoto Metric Determination of Aminopline, Trazodone, Amitriptyline through Using Methyl Orange and Bromocresol Green Reagent,” Spectrochemi Acta Part A, Vol. 65, 2006, pp. 26-30.
[12] A. Tehssen, A. A Kazi, I. H. Mohamed and A. Tal, “Spectrophotometric Determination of Amitriptyline Hydrochloride in Pure and Pharmaceutical Preparation,” Analytical letters, Vol. 33, No. 12, 2000, pp. 2477-2490. doi:10.1080/00032710008543203
[13] J. O. Onah, “Spectrophotometric Determination of Amitriptyline by Method of Charge Transfer Complexation with Chloranic,” Acid Global Journal of Puro and Applied Science, Vol. 11, No. 2, 2005.
[14] W. Micink and M. Tarasiewicz, “Application of Thiocynate Complex of Titanium (iv) to the Extractive Spectrophotometric Determination of Amitriptyline Hydrochloride,” Microchemical Journal, Vol. 78, No. 107, 1993, pp. 79-88,
[15] R. M. El-Nashar, N. T. Ghani Abdel and A. A. Bioumy, “Flow Injection Potentiometric Determination of Amitriptyline Hydrochloride,” Microchemical Journal, Vol. 78, No. 2, 2004, pp. 107-113. doi:10.1016/j.microc.2004.03.002
[16] E. M. Elnemma, F. M. ElZawawy and S. M. Hassan, “Saad Determination of Amitriptyline Impiramine and Orphenadrine in Antidepressant Drug by Potentiometry, Spectrometry, and Atomic Absorption Spectrophotometry,” Microchimica Acta, Vol. 110, No. 13, 1993, pp. 79-88. doi:10.1007/BF01243988
[17] T. Ghani Nour, A. El. Nashar, R. M. Bioumy and A. Abeer, “Conductimetric Determination of the Antidepressant Amitriptyline and Dothiepin Hydrochloride and Tranylcypromine Hemisulphate in their pharmaceutical formulation,” FABAD Journal of Pharmaceutical Sciences, Vol. 29, 2004, pp. 195-201
[18] C. Jessica, R. Alberto, A. Emillio and J. Elena, “Temperature Effect on the Complex Formation between Tricyclic Antidepressant Drug (Amitriptyline or Imipramine and Hydroxypropyl-β-cyclo-dextrine in Water,” J. Incl. phenom Macrocycl che. 2007
[19] G. V. Deshmane, J. R. Vakil, S. R. Dhaheshwar, K. R. Mahadik and S. S. Kadam, “Simultaneous Spectrophotometric Estimation of Amitriptyline Hydrochloride and Chlordiazepoxide,” Indian Drug, Vol. 34, 1997, pp. 443-445.
[20] R. Gandhi, P. Sharma, H. P. S. Chawla, C. L. Kaul and R. Panchagnula, “HPLC Analysis of Tricyclic Antidepressant in Skin Diffusion Studies,” S.S.P. Pharmaceutical Science, Vol. 10, 2000, pp. 234-238.
[21] W. Lu, S. A. Shamsi, T. D. McCarley and I. M. Warner, “Online Capillary Electrophorosis Electrospray Ionization Mass Spectroscopy Using Polymerized Anionic Surfactant,” Electrophoresis, Vol. 19, No. 12, 1998, pp. 2193-2199. doi:10.1002/elps.1150191225
[22] L. Simonson and K. Nelson, “Adsorption of Amitriptyline Hydrochloride in HPLC Injector Loops,” LC-GC, Vol. 10, No. 7, 1992, pp. 533-535.
[23] E. Janquera, S. Romero and C. Aicart, “Behavavior of Tricyclic Antidepressanls in Aqueous Solution: Seif Aggregation and Association with β-Cyclodextrin,” Langmuir, Vol. 17, No. 6, 2001, pp. 1826-1832.

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.