Covariation of mutation pairs expressed in HIV-1 protease and reverse transcriptase genes subjected to varying treatments
David King, Roger Cherry, Wei Hu
DOI: 10.4236/jbise.2010.33039   PDF   HTML     5,268 Downloads   8,651 Views   Citations


A previous study, focused on the correlation of muta-tion pairs of synonymous (S) and asynonymous (A) mutations, distinguished only between the treated and untreated data of protease and reverse tran-scriptase (RT) of HIV-1 subtype B. It is well known that single mutation patterns in HIV-1 are treat-ment-specific. It logically follows that covariation between mutations will also be treatment specific. Thus, our motivation is to give a more in depth study of the covariation between mutation pairs, analyzing not only treated and untreated, but what specific treatments were used, and how they affected the co-variation between the mutations differently. We in-tended to further deepen this study by analyzing the covariation of mutations in protease and RT in dif-ferent subtypes of HIV-1. We found that virus sam-ples subjected to antiretroviral Protease- and RT- inhibitors do show different patterns of mutation covariation in B-subtype protease and RT of HIV-1, while maintaining the same overall trend. covariation will tend to be higher and more distinct from and covariation after treatment. The same trend continues in protease and RT re-gardless of subtype. We also found the highly cova-ried codon positions, position pairs, and position- covariation clusters in protease, affected by different treatments. Most of them are well known major drug-resistance sites for these treatments.

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King, D. , Cherry, R. and Hu, W. (2010) Covariation of mutation pairs expressed in HIV-1 protease and reverse transcriptase genes subjected to varying treatments. Journal of Biomedical Science and Engineering, 3, 291-299. doi: 10.4236/jbise.2010.33039.

Conflicts of Interest

The authors declare no conflicts of interest.


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