A new size and shape controlling method for producing calcium alginate beads with immobilized proteins

Yan Zhou, Shin’ichiro Kajiyama, Hiroshi Masuhara, Yoichiro Hosokawa, Takahiro Kaji, Kiichi Fukui

Department of Applied Physics, Grad. School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Osaka, Japan.
Department of Biotechnology, Grad. School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Osaka, Japan;.

DOI: 10.4236/jbise.2009.25043                

Abstract

A method for producing size- and shape-con-trolled calcium alginate beads with immobilized proteins was developed. Unlike previous cal-cium alginate bead production methods, pro-tein-immobilized alginate beads with uniform shape and sizes less then 20 micrometers in diameter could successfully be produced by using sonic vibration. BSA and FITC-conjugated anti-BSA antibodies were used to confirm pro-tein immobilization in the alginate beads. Pro-tein diffusion from the beads could be reduced to less than 10% by cross-linking the proteins to the alginate with 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxysul-fosuccinimide (NHSS). The calcium alginate beads could also be arranged freely on a slide glass by using a femtosecond laser.

Keywords

Calcium Alginate Beads; Size Controllable Production Method; Protein Immobilized Beads; Femtosecond Laser; Laser Manipulation

Conflicts of Interest

The authors declare no conflicts of interest.

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