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Dynamics and Activity of Sulfate-Reducing Bacterial Populations in Paddy Soil under Subsurface Drainage: Case Study of Kamboinse in Burkina Faso

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DOI: 10.4236/as.2015.611135    3,202 Downloads   3,595 Views  


Sulfide toxicity is a common disease generally associated with iron toxicity which occurs in rice fields when the Sulfate-Reducing Bacteria (SRB) produce sulfides ions in anaerobic conditions. The high quantity of sulfides ions in the soil solution upsets the mineral element balance in the rice, affects its growth and causes crop yield losses. In Burkina Faso, many rice field soils are abandoned due to sulfides toxicity. The present study was developed to evaluate the impact of subsurface drainage on SRB dynamics and activity during rice cultivation and the incidence on rice production. Twelve concrete microplots with a clay-loam soil and a rice variety susceptible to sulfides toxicity (FKR 19) were used for the experiment. Soil in microplots was drained for 7 days (P1), 14 days (P2), and 21 days (P3), respectively. Control (T) microplots without drainage were prepared similarly. The evolution of SRB populations and the content of sulfides ions in the paddy soil and in soil near rice roots were monitored throughout the cultural cycle using MPN and colorimetric methods, respectively. Data obtained were analyzed in relation to drainage frequency, rice growth stage, and rice yield using the Student’s t-test and XLSTAT 7.5.2 statistical software. From the results obtained, the subsurface drainage did not affect significantly SRB populations (P = 0.187). However, the drainage affected significantly sulfides concentration in the soil near rice roots (P = 0.032). The concentration of sulfides (P < 0.0001) in soil near rice roots and the number of SRB (P < 0.0001) were significantly higher during the rice tillering and maturity stages. Although no significant difference was observed for rice yield among treatments (P = 0.209), the P2 subsurface drainage showed the highest yield and a low concentration of sulfides in soil near rice roots.

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Otoidobiga, C. , Keita, A. , Yacouba, H. , Traore, A. and Dianou, D. (2015) Dynamics and Activity of Sulfate-Reducing Bacterial Populations in Paddy Soil under Subsurface Drainage: Case Study of Kamboinse in Burkina Faso. Agricultural Sciences, 6, 1393-1403. doi: 10.4236/as.2015.611135.


[1] Muyzer, G. and Stams, A.J.M. (2008) The Ecology and Biotechnology of Sulphate-Reducing Bacteria. Nature Reviews Microbiology, 6, 441-456.
[2] Freney, L.R., Jacq, V.A. and Baldensperger, L.F. (1982) The Significance of the Biological Sulfur Cycle in Rice Production. In: Dommergues, Y.R. and Diem, H.G., Eds., Microbiology of Tropical Soils and Plant Productivity, Nijhoff/Lunk, Hague, 271-317.
[3] Lücker, S., Steger, D., Kjeldsen, K.U., MacGregor, B.J., Wagner, M. and Loy, A. (2007) Improved 16S rRNA-Targeted Probe Set for Analysis of Sulfate-Reducing Bacteria by Fluorescence in Situ Hybridization. Journal of Microbiological Methods, 69, 523-528.
[4] Shibata, A., Toyota, K., Miyake, K. and Katayama, A. (2007) Anaerobic Biodegradation of 4-Alkylphenols in a Paddy Soil Microcosm Supplemented with Nitrate. Chemosphere, 68, 2096-2103.
[5] Yang, S.Y., Yoshida, N., Baba, D. and Katayama, A. (2008) Anaerobic Biodegradation of Biphenyl in various Paddy Soils and River Sediment. Chemosphere, 71, 328-336.
[6] Liu, X.-Z., Zhang, L.-I., Prosser, J.I. and He, J.-Z. (2009) Abundance and Community Structure of Sulfate Reducing Prokaryotes in a Paddy Soil of Southern China under Different Fertilization Regimes. Soil Biology and Biochemistry, 41, 687-694.
[7] Jorgensen, B.B. (1982) Mineralization of Organic-Matter in the Sea Bed: The Role of Sulfate Reduction. Nature, 296, 643-645.
[8] Gilmour, C.C., Riedel, G.S., Ederington, M.C., Bell, J.T., Benoit, J.M., Gill, G.A. and Stordal, M.C. (1998) Methylmercury Concentrations and Production Rates across a Trophic Gradient in the Northern Everglades. Biogeochemistry, 40, 327-345.
[9] Habicht, K.S. and Canfield, D.E. (1996) Sulphur Isotope Fractionation in Modern Microbial Mats and the Evolution of the Sulphur Cycle. Nature, 382, 342-343.
[10] Castro, H., Reddy, K.R. and Ogram, A. (2002) Composition and Function of Sulfate-Reducing Prokaryotes in Eutrophic and Pristine Areas of the Florida Everglades. Applied and Environmental Microbiology, 68, 6129-6137.
[11] Schmalenberger, A., Drake, H.L. and Küsel, K. (2007) High Unique Diversity of Sulfate-Reducing Prokaryotes Characterized in a Depth Gradient in an Acidic Fen. Environmental Microbiology, 9, 1317-1328.
[12] Bao, P., Hu, Z.Y, Wang, X.J., Chen, J., Ba, Y.X., Hua, J., Zhu, C.Y., Zhong, M. and Wu, C.Y. (2012) Dechlorination of p,p’-DDTs Coupled with Sulfate Reduction by Novel Sulfate-Reducing Bacterium Clostridium sp. BXM. Environmental Pollution, 162, 303-310.
[13] Kondo, R., Nedwell, D.B., Purdy, K.J. and Silva, S.Q. (2004) Detection and Enumeration of Sulphate-Reducing Bacteria in Estuarine Sediments by Competitive PCR. Geomicrobiology Journal, 21, 145-157.
[14] Leloup, J., Quillet, L., Berthe, T. and Petit, F. (2005) Diversity of the dsrAB (Dissimilatory Sulfite Reductase) Gene Sequences Retrieved from Two Contrasting Mudflats of the Seine Estuary, France. FEMS Microbiology Ecology, 55, 230-238.
[15] Foti, M., Sorokin, D.Y., Lomans, B., Mussman, M., Zacharova, E.E., Pimenov, N.V., Kuenen, J.G. and Muyzer, G. (2007) Diversity, Activity, and Abundance of Sulfate-Reducing Bacteria in Saline and Hypersaline Soda Lakes. Applied and Environmental Microbiology, 73, 2093-2100.
[16] Stubner, S. (2004) Quantification of Gram-Negative Sulphate-Reducing Bacteria in Rice Field Soil by 16S rRNA Gene-Targeted Real-Time PCR. Journal of Microbiological Methods, 57, 219-230.
[17] Peck Jr., H.D. (1993) Bioenergetic Strategies of the Sulfate-Reducing Bacteria. In: Odom, J.M. and Singleton Jr., R., Eds., The Sulfate Reducing Bacteria: Contemporary Perspectives, Springer-Verlag, New York, 41-76.
[18] Ouattara, A.S. (1992) Contribution to the Study of Iron-Reducing Bacteria and Sulfate in Paddy Soils of the Kou Valley (Burkina Faso). PhD Dissertation, University of Provence Aix-Marseille I, Aix-en-Provence.
[19] Dianou, D. and Traoré, S.A. (2000) Sulfate-Reducing Bacterial Populations in Some Lowland Paddy Field Soils of Burkina Faso (West Africa). Microbes and Environments, 15, 41-44.
[20] Jacq, V. (1975) The Sulfate-Reduction in Relation with the Root Excretion. Bulletin de la Société Botanique de France, 122, 169-181.
[21] Dobermann, A. and Fairhurst, T. (2000) Rice: Nutrient Disorders and Nutrient Management. International Rice Research Institute, Los Baños, 191.
[22] Ling, Y.-C., Bush, R., Grice, K., Tulipani, S., Berwick, L. and Moreau, J.W. (2015) Distribution of Iron- and Sulfate-Reducing Bacteria across a Coastal Acid Sulfate Soil (CASS) Environment: Implications for Passive Bioremediation by Tidal Inundation. Frontiers in Microbiology, 6, 1-15.
[23] Jacq, V.A. (1977) Rice Susceptibility to Microbial Sulfides. ORSTOM Book, Biology Serial, 12, 97-99.
[24] Escoffier, S., Olliver, B., Le Mer, J., Garcin, J. and Roger, P. (1998) Evidence and Quantification of Thiosulfate Reducers Unable to Reduce Sulfate in Rice Field Soils. European Journal of Soil Biology, 34, 69-74.
[25] Otoidobiga, C.H., Keita, A., Yacouba, H., Traore, A.S. and Dianou, D. (2015) Dynamics and Activity of Iron-Reducing Bacterial Populations in a West African Rice Paddy Soil under Subsurface Drainage: Case Study of Kamboinse in Burkina Faso. Agricultural Sciences, 6, 860-869.
[26] Keïta, A. (2015) Subsurface Drainage of Valley Bottom Irrigated Rice Schemes in Tropical Savannah: Case Studies of Tiefora and Moussodougou in Burkina Faso. PhD Thesis, Wageningen University, Delft.
[27] INERA (2000) Descriptive Folders of Rice Cultivars. Institute of Environment and Agricultural Research, Ouagadougou.
[28] Sokona, M.E.B., Boro, A., Hema, A. and Katiella, B. (2010) Diagnostic Study of the Rice Irrigation Scheme of Tiefora, Province of Comoe, Region of the Cascades. Field Report, 2iE, Ouagadougou.
[29] Widdel, F. (1983) Methods for Enrichment and Pure Culture Isolation of Filamentous Gliding Sulfate-Reducing Bacteria. Archives of Microbiology, 134, 282-285.
[30] Cord-Ruwisch, R. (1985) A Quick Method for the Determination of Dissolved and Precipitated Sulfides in Cultures of Sulfate-Reducing Bacteria. Journal of Microbiological Methods, 4, 33-36.
[31] Jacq, V.A. (1978) Adaptation of the Colorimetric Method of Chaudhry and Cornfield to the Dosage of Dissolved Sulfides in a Waterlogged Soil. ORSTOM Book, Biology Serial, 13, 129-132.
[32] Dianou, D., Lopes, J., Traoré, S.A., Lino, A., Moura, I. and Moura, J.G. (1998) Characterization of Desulfovibrio sp. Isolated from Some Lowland Paddy Field Soils of Burkina Faso. Soil Science and Plant Nutrition, 44, 459-465.
[33] Garcia, J.L., Rainbault, M., Jacq, V., Rinaudo, G. and Roger, P. (1974) Microbial Activities in Paddy Soils of Senegal: Relations with Physicochemical Characteristics and Influence of the Rhizosphere. Journal of Ecology and Soil Biology, 11, 169-185.
[34] Loyer, J.Y., Jacq, V.A. and Reynaud, P.A. (1982) Physicochemical Variations in a Flooded Rice Field Soil and Evolution of the Algal Biomass and Microbial Populations in the Sulfur Cycle. ORSTOM Book, Biology Serial, 45, 53-72.
[35] Bongoua, D.A.J. (2009) Iron-Reducing Bacterial Communities Implications and Environmental Settings in the Functioning and Rice Fields Soils Quality (Thaïlande and Ivoiry Coast). PhD Thesis, Henri Poincare University, Nancy.
[36] Wind, T., Stubner, S. and Conrad, R. (1999) Sulfate-Reducing Bacteria in Rice Field Soil and on Rice Roots. Systematic and Applied Microbiology, 22, 269-279.
[37] Wind, T. and Conrad, R. (1997) Localization of Sulfate Reduction in Planted and Unplanted Rice Field Soil. Biogeochemistry, 37, 253-278.
[38] Jacq, V.A., Prade, K. and Ottow, L.C.G. (1991) Iron Sulphide Accumulation in the Rhizosphere of Wetland Rice (Oryza sativa L.) as the Result of Microbial Activities. In: Berthelin, J., Ed., Diversity of Environmental Biogeochemistry, Developments in Geochemistry, Vol. 6, Elsevier, Amsterdam, 453-468.
[39] Amann, R.L., Ludwing, W. and Schleifer, K.H. (1995) Phylogenetic Identification and in Situ Detection of Individual Microbial Cells without Cultivation. Microbiological Reviews, 59, 143-169.
[40] Scavino, A.F., Menes, J., Ferrando, L. and Tarlera, S. (2010) Bacterial Community Analysis of the Water Surface Layer from a Rice-Planted and an Unplanted Flooded Field. Brazilian Journal of Microbiology, 41, 411-419.
[41] Ito, T., Nielsen, J.L., Okabe, S., Watanabe, Y. and Nielsen, P.H. (2002) Phylogenetic Identification and Substrate Uptake Patterns of Sulfate-Reducing Bacteria Inhabiting an Oxic-Anoxic Sewer Biofilm Determined by Combining Microautoradiography and Fluorescent in Situ Hybridization. Applied and Environmental Microbiology, 68, 356-364.
[42] Postgate, J.R. (1984) The Sulfate-Reducing Bacteria. 2nd Edition, Cambridge University Press, Cambridge, United Kingdom.
[43] Prade, K. (1987) Influence of Nutrient Supply to the Iron Poisoning of Paddy (O. sativa L.) in the Basse Casamance Senegal. PhD Thesis, University of Hohenheim, Stuttgart. (In German)
[44] Johnson, M.S., Zhulin, I.B., Gapuzan, M.-E.R. and Taylor, B.L. (1997) Oxygen Dependent Growth of the Obligate Anaeorobe Desulfovibrio vulgaris Hildenborough. Journal of Bacteriology, 179, 5598-5601.
[45] Dolla, A., Fournier, M. and Dermoun, Z. (2006) Oxygen Defense in Sulfate-Reducing Bacteria. Journal of Biotechnology, 126, 87-100.
[46] Ravenschalag, K., Sahm, K., Knoblauch, C., Jorgensen, B.B. and Amann, R. (2000) Community Structure, Cellular rRNA Content and Activity of Sulfate Reducing Bacteria in Marine Arctic Sediment. Applied and Environmental Microbiology, 66, 3592-3602.
[47] Mussmann, M., Ishii, K., Rabus, R. and Amann, R. (2005) Diversity and Vertical Distribution of Cultured and Uncultured Deltaproteobacteria in an Interdital Mud Flat of the Wadden Sea. Environmental Microbiology, 7, 405-418.
[48] Sigalevich, P., Meshorer, E., Helmann, Y. and Cohen, Y. (2000) Transition from the Anaerobic to Aerobic Conditions for the Sulfate-Reducing Bacterium Desulfovidrio oxyclinea Results in Flocculation. Applied and Environmental Microbiology, 66, 5005-5012.
[49] Liesack, W., Shnell, S. and Revsbech, N.P. (2000) Microbiology of Flooded Rice Bodies. FEMS Microbiology Reviews, 24, 625-645.
[50] Ponnamperuma, F.N. (1972) The Chemistry of Submerged Soils. Advances in Agronomy, 24, 29-96.
[51] IRRI (2002) Standard Evaluation System for Rice. International Rice Research Institute, Manila.
[52] Vàmos, R. (1959) “Brusone” Disease of Rice in Hungary. Plant and Soil, 11, 65-77.
[53] Baba, I. and Iwata, I. (1963) Akiochi Disease of Rice Plants. In: Matsubayashi, M., Ito, R., Nomoto, T., Takase, T. and Yamada, N., Eds., Theory and Practice of Growing Rice, Fuji Publishing Co. Ltd., Tokyo, 149-158.
[54] Takijima, Y. (1965) Studies on the Mechanism of Root Damage of Rice Plants in the Paddy Fields (Part 1). Root Damage and Growth Inhibitory Substances Found in the Peaty and Peat Soil. Soil Science and Plant Nutrition, 10, 1-8.
[55] Armstrong, J. and Armstrong, W. (2005) Rice: Sulfide-Induced Barriers to Root Radial Oxygen Loss, Fe2+ and Water Uptake, and Lateral Root Emergence. Annals of Botany, 96, 625-638.
[56] Mitsui, S. (1965) Dynamic Aspects of Nutrient Uptake. In: Tanaka, A., Ed., The Mineral Nutrition of the Rice Plant, Johns Hopkins University Press, Baltimore, 53-62.
[57] Tanaka, A., Ranjit, P., Mulleriyawa, R.P. and Yasu, T. (1968) Possibility of Hydrogen Sulphide Induced Iron Toxicity of the Rice Plant. Soil Science and Plant Nutrition, 14, 1-6.
[58] Allam, A.I. and Hollis, J.P. (1972) Sulphide Inhibition of Oxidases in Rice Roots. Phytopathology, 62, 634-639.
[59] Joshi, M.M., Ibrahim, I.K.A. and Hollis, J.P. (1975) Hydrogen Sulphide: Effects on the Physiology of Rice Plants and Relation to Straighthead Disease. Phytopathology, 65, 1165-1170.
[60] Takijima, Y. (1964) Studies on the Mechanism of Root Damage of Rice Plants in the Peat Paddy Fields. Soil Science and Plant Nutrition, 11, 20-27.
[61] Fageria, N.K., Santos, A.B., Barbosa, F.M.P. and Guimarães, C.M. (2008) Iron Toxicity in Lowland Rice. Journal of Plant Nutrition, 31, 1676-1697.
[62] Ottow, J.C.G., Prade, K., Bertenbreiter, W. and Jacq, V.A. (1993) Iron Toxicity Mechanisms of Flooded Rice (Oryza sativa L.) in Senegal and Indonesia. In: Raunet, M., Ed., Lowland and Rice-Growing, CIRAD-CA, Montpellier, 231-241.
[63] Ethan, S., Odunze, A.C., Abu, S.T. and Iwuafor, E.N.O. (2011) Effect of Water Management and Nitrogen Rates on Iron Concentration and Yield in Lowland Rice. Agriculture and Biology Journal of North America, 2, 622-629.
[64] Muhrizal, S., Shamshuddin, J., Fauziah, I. and Husni, M.H.A. (2003) Alleviation of Aluminum Toxicity in Acid Sulfate Soils in Malaysia Using Organic Materials. Communications in Soil Science and Plant Analysis, 34, 2999-3017.
[65] Suswanto, T., Shamshuddin, J., Syed Omar, S.R., Mat, P. and Teh, C.B.S. (2007) Alleviating an Acid Sulfate Soil Cultivated to Rice (Oryza sativa) Using Ground Magnesium Limestone and Organic Fertilizer. Jurnal Tanah dan Lingkungan, 9, 1-9.
[66] Hauck, S., Benz, M., Brune, A. and Schink, B. (2001) Ferrous Iron Oxidation by Denitrifying Bacteria in Profundal Sediments of a Deep Lake (Lake Constance). FEMS Microbiology Ecology, 37, 127-134.
[67] Wind, T. and Conrad, R. (1995) Sulfur Compounds, Potential Turnover of Sulfate Reduction and Thiosulfate, and Numbers of Sulfate-Reducing Bacteria in Planted and Unplanted Paddy Soil. FEMS Microbiology Ecology, 18, 257-266.

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