Bioinspired-Interpenetrating Network (IPNs) Hydrogel (BIOF-INPs) and TMD in Vitro: Bioadhesion, Drug Release and Build in Free Radical Detection and Defense

DOI: 10.4236/ojst.2015.53008   PDF   HTML   XML   2,540 Downloads   2,996 Views   Citations

Abstract

In this work, Bioactive-functionalized interpenetrating network (IPNs) hydrogel (BIOF-INPs) were prepared and investigated in vitro for the free radical detection/defense, therapeutic release as well as shear bond strength to dentine, ability to re-mineralize surface of the dentin after application of these bio-inspired materials using a biologically inspired mineralization process in vitro as well as investigating antimicrobial properties of the BIOF-INPs against S. aureous. The aim of this investigation was to evaluate the suitability and flexibility of the designer materials to act as an “in vitro” probe to gain insights into molecular origin of TMD and associated disorders.

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Perchyonok, V. , Reher, V. , Basson, N. , Zhang, S. and Grobler, S. (2015) Bioinspired-Interpenetrating Network (IPNs) Hydrogel (BIOF-INPs) and TMD in Vitro: Bioadhesion, Drug Release and Build in Free Radical Detection and Defense. Open Journal of Stomatology, 5, 53-61. doi: 10.4236/ojst.2015.53008.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] McCullen, S.D., Chow, A.G. and Stevens, M.M. (2011) In Vivo Tissue Engineering of Musculoskeletal Tissues. Current Opinion in Biotechnology, 22, 715-720.
http://dx.doi.org/10.1016/j.copbio.2011.05.001
[2] Panseri, S., Russo, A., Cunha, C., Bondi, A., Di Martino, A., Patella, S. and Kon, E. (2012) Osteochondral Tissue Engineering Approaches for Articular Cartilage and Subchondral Bone Regeneration. Knee Surgery, Sports Traumatology, Arthroscopy, 20, 1182-1191.
http://dx.doi.org/10.1007/s00167-011-1655-1
[3] Milam, S.B. and Schmitz, J.P. (1995) Molecular Biology of Temporo-Mandibular Joint Disorders: Proposed Mechanisms of Disease. Journal of Oral and Maxillofacial Surgery, 53, 1448-1454.
http://dx.doi.org/10.1016/0278-2391(95)90675-4
[4] Chan, P.H. (2001) Reactive Oxygen Radicals in Signaling and Damage in the Ischemic Brain. Journal of Cerebral Blood Flow & Metabolism, 21, 2-14.
http://dx.doi.org/10.1097/00004647-200101000-00002
[5] Irmak, MK., Fadillioglu, E. and Gülec, M. (2002) Effects of Electromagnetic Radiation from a Cellular Telephone on the Oxidant and Antioxidant Levels in Rabbits. Cell Biochemistry and Function, 20, 279-283.
http://dx.doi.org/10.1002/cbf.976
[6] Yaser, M.M., Randa, M.M. and Belacy, A. (2001) Effects of Acute Exposure to the Radiofrequency Fields of Cellular Phones on Plasma Lipid Peroxide and Antioxidase Activities in Human Erythrocytes. Journal of Pharmaceutical and Biomedical Analysis, 26, 605-608.
http://dx.doi.org/10.1016/S0731-7085(01)00492-7
[7] Pompella, A. (1997) Biochemistry and Histochemistry of Oxidant Stress and Lipid Peroxidation. International Journal for Vitamin and Nutrition Research, 67, 289-297.
[8] Sumii, H., Inoue, H., Onoue, J., Mori, A., Oda, T. and Tsubokura, T. (1996) Superoxide Dismutase Activity in Arthropathy: Its Role and Measurement in the Joints. Hiroshima Journal of Medical Sciences, 45, 51-55.
[9] Arinzeh, T.L., Tran, T., McAlary, J. and Daculsi, G. (2005) A Comparative Study of Biphasic Calcium Phosphate Ceramics for Human Mesenchymal Stem-Cell-Induced Bone Formation. Biomaterials, 26, 3631-3638.
http://dx.doi.org/10.1016/j.biomaterials.2004.09.035
[10] Holmlund, A.B. and Axelsson S. (1996) Temporomandibular Arthropathy: Correlation between Clinical Signs and Symptoms and Arthroscopic Findings. International Journal of Oral & Maxillofacial Surgery, 25, 266-271.
http://dx.doi.org/10.1016/S0901-5027(96)80024-5
[11] Emshoff, R. (2005) Clinical Factors Affecting the Out-come of Arthrocentesis and Hydraulic Distension of the Temporomandibular Joint. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 100, 409-414.
http://dx.doi.org/10.1016/j.tripleo.2004.12.024
[12] Voog, ü., Alstergren, P., Eliasson, S., Leibur, E., Kallikorm, R. and Kopp, S. (2003) Inflammatory Mediators and Radiographic Changes in Temporomandibular Joints in Patients with Rheumatoid Arthritis. Acta Odontologica Scandinavica, 61, 57-64.
[13] Kim, S.J., Park, Y.H., Hong, S.P., Cho, B.O., Park, J.W. and Kim, S.G. (2003) The Presence of Bacteria in the Synovial Fluid of the Temporomandibular Joint and Clinical Significance: Preliminary Study. Journal of Oral and Maxillofacial Surgery, 61, 1156-1161.
http://dx.doi.org/10.1016/S0278-2391(03)00674-8
[14] Paegle, D.I., Holmlund, A.B., öStlund, M.R. and Grillner, L. (2004) The Occurrence of Antibodies against Chlamydia Species in Patients with Monoarthritis and Chronic Closed Lock of the Temporomandibular Joint. Journal of Oral and Maxillofacial Surgery, 62, 435-439.
http://dx.doi.org/10.1016/j.joms.2003.08.020
[15] Leibur, E., Jagur, O., Müürsepp, P., Veede, L. and Voog-Oras, ü. (2010) Long-Term Evaluation of Arthroscopic Surgery with Lysis and Lavage of Temporomandibular Disorders. Journal of Cranio-Maxillofacial Surgery, 38, 615-620.
http://dx.doi.org/10.1016/j.jcms.2010.02.003
[16] Alstergren, P., Kopp, S. and Theordosson, E. (2003) Synovial Fluid Sampling from the Temporomandibular Joint: Sample Quality Criteria and Levels of Interleukin-1 Beta and Serotonin. Acta Odontologica Scandinavica, 57, 278-282.
[17] Kamada, A., Kakudo, K., Arika, T., Okazaki, J., Kano, M. and Sakaki, T. (2000) Assay of Synovial MMP-3 in Temporomandibular Joint Diseases. Journal of Cranio-Maxillofacial Surgery, 28, 247-248.
[18] Alstergren, P. and Kopp, S. (1997) Pain and Synovial Fluid Concentration in Arthritic Temporomandibular Joints. Pain, 72, 137-143.
[19] Perchyonok, V., Zhang, S. and Oberholzer, T. (2011) Towards Development of Novel Chitosan Based Drug Delivery Prototypes: Devices for Targeted Delivery Drug Therapy at the Molecular Level in Aqueous Media. Current Organic Chemistry, 16, 2437-2439.
[20] Perchyonok, V.T., Reher, V., Zhang, S., Grobler, S.R., Oberholzer, T.G. and Massey, W. (2014) Insights and Relative Effect of Aspirin, Naproxen and Ibuprofen Containing Hydrogels: From Design to Performance as a Functional Dual Capacity Restorative Material and Build in Free Radical Defense: In-Vitro Studies. Open Journal of Stomatology, 4, 73-83.
http://dx.doi.org/10.4236/ojst.2014.42013
[21] Perchyonok, V., Zhang, S. and Oberholzer, T. (2012) Novel Melatonin-Chitosan Hydrogels as Suitable Oral Bio-Drug Delivery Systems to Fight Oral Mucositis: Synergy of Antioxidants and Bioactives in Action. Current Organic Chemistry, 16, 2430-2436.
[22] Perchyonok, V.T., Zhang, S., Grobler, S.R., Oberholzer, T.G. and Massey, W. (2014) Insights into and Relative Effect of Chitosan-Krill Oil, Chitosan-H-Aspirin, Chitosan-H-Krill Oil-Nystatin and Chitosan-H-Krill Oil-Aspirin-Nystatin on Dentin Bond Strength and Functional Drug Delivery Capacity: In-Vitro Studies. European Journal of General Dentistry, 3, 57-65.
[23] Perchyonok, V.T., Zhang, S., Basson, N.J., Grobler, S.R., Oberholzer, T.G. and Massey, W. (2014) Insights into Functional Erythromycin/Antioxidant Containing Chitosan Hydrogels as Potential Bio-Active Restorative Materials: Structure, Function and Antimicrobial Activity. Advanced Techniques in Biology and Medicine, 2, 116.
[24] Perchyonok, V.T., Zhang, S., Basson, N.J., Grobler, S.R., Oberholzer, T.G. and Massey, W. (2014) Insights into Functional Tetracycline/Antioxidant Containing Chitosan Hydrogels as Potential Bio-Active Restorative Materials: Structure, Function and Antimicrobial Activity. Open Journal of Stomatology, 4, 99-108.
http://dx.doi.org/10.4236/ojst.2014.43016
[25] Perchyonok, V.T., Zhang, S., Grobler, S.R. and Oberholzer, T.G. (2013) Insights into and Relative Effect of Chitosan-H, Chitosan-H-Propolis, Chitosan-H-Propolis-Nystatin and Chitosan-H-Nystatin on Dentine Bond Strength. European Journal of Dentistry, 7, 412-418.
[26] Tampieri, A., Celotti, G., Landi, E., Sandri, M., Roveri, N. and Falini, G. (2003) Biologically Inspired Synthesis of Bone-Like Composite: Self-Assembled Collagen Fibers/Hydroxyapatite Nanocrystals. Journal of Biomedical Materials Research Part A, 67, 618-625.
[27] Tampieri, A., Sandri, M., Landi, E., Pressato, D., Francioli, S., Quarto, R. and Martin, I. (2008) Design of Graded Biomimetic Osteochondral Composite Scaffolds. Biomaterials, 29, 3539-3546.
http://dx.doi.org/10.1016/j.biomaterials.2008.05.008
[28] Zhao, H., Wang, G., Hu, S., Cui, J., Ren, N., Liu, D., Liu, H., Cao, C., Wang, J. and Wang, Z. (2011) In Vitro Biomimetic Construction of Hydroxyapatite-Porcine Acellular Dermal Matrix Composite Scaffold for MC3T3-E1 Preosteoblast Culture. Tissue Engineering Part A, 17, 765-776.
http://dx.doi.org/10.1089/ten.tea.2010.0196
[29] Bernhardt, A., Lode, A., Boxberger, S., Pompe, W. and Gelinsky, M. (2008) Mineralised Collagen—An Artificial, Extracellular Bone Matrix—Improves Osteogenic Differentiation of Bone Marrow Stromal Cells. Journal of Materials Science: Materials in Medicine, 19, 269-275.
http://dx.doi.org/10.1007/s10856-006-0059-0
[30] Domaschke, H., Gelinsky, M., Burmeister, B., Fleig, R., Hanke, T., Reinstorf, A., Pompe, W. and Rosen-Wolff, A. (2006) In Vitro Ossification and Remodeling of Mineralized Collagen I Scaffolds. Tissue Engineering, 12, 949-958.
[31] Hou, R., Zhang, G., Du, G., Zhan, D., Cong, Y., Cheng, Y. and Fu, J. (2013) Magnetic Nanohydroxyapatite/PVA Composite Hydrogels for Promoted Osteoblast Adhesion and Proliferation. Colloids and Surfaces B: Biointerfaces, 103, 318-325.
http://dx.doi.org/10.1016/j.colsurfb.2012.10.067
[32] Mertens, M.E., Hermann, A., Buhren, A., Olde-Damink, L., Möckel, D., Gremse, F., Ehling, J., Kiessling, F. and Lammers, T. (2014) Iron Oxide-Labeled Collagen Scaffolds for Non-Invasive MR Imaging in Tissue Engineering. Advanced Functional Materials, 24, 754-762.
[33] Almeida, J.P.M., Chen, A.L., Foster, A. and Drezek, R. (2011) In Vivo Biodistribution of Nanoparticles. Nanomedicine, 6, 815-835.
http://dx.doi.org/10.2217/nnm.11.79
[34] Mahmoudi, M., Hofmann, H., Rothen-Rutishauser, B. and Petri-Fink, A. (2012) Assessing the in Vitro and in Vivo Toxicity of Super-Paramagnetic Iron Oxide Nanoparticles. Chemical Reviews, 112, 2323-2338.
http://dx.doi.org/10.1021/cr2002596

  
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