In Vitro Somatic Embryogenesis in Some Oil Yielding Tropical Tree Species

Premananda Das

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DOI: 10.4236/ajps.2011.22023                

Abstract

Somatic embryogenesis was achieved in two oil yielding tropical tree species i.e. Simarouba glauca & Azadirachta indica using immature zygotic embryos as explants on Murashige and Skoog (MS) medium supplemented with 0.5 – 1.5 mg/l benzylaminopurine (BA) and 2.0 - 3.0 mg/l NAA (1-napthaleneacetic acid) or 2, 4-D (2,4-dichlorophenoxyacetic acid) and 3% sucrose. MS medium containing 1.0 mg/l BA and 2.0 mg/l NAA was noted to be the most effective in inducing friable embryogenic callus (FEC) in Simarouba glauca; the number of somatic embryos per culture varied in MS medium supplemented with 1.0 – 1.5 mg/l BA and 1.0 mg/l NAA. In Azadirachta indica, somatic embryos developed on MS medium supplemented with 0.5 mg/l BA and 1.5 – 2.0 mg/l 2,4-D which were in various shapes and sizes after the first subculture on MS medium supplemented with 0.25 mg/l abscisic acid. The somatic embryos which developed shoots were isolated and rooted in 1/2 strength MS medium supplemented with 0.25 mg/l abscisic acid and 2% sucrose. About 25% of embryos germinated within 20 days of culture in case of Simarouba glauca and 62% in Azadirachta indica. The somatic embryo-derived plantlets were transferred to the field after being hardened in the climate controlled hardening chamber.

Keywords

Somatic Embryogenesis, Immature Zygotic Embryos, Growth Regulators, Oil Yielding Tropical Tree

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Anonymous. The Wealth of India:A Dictionary of Indian Raw Material and industrial Products. Vol. 1, 1985, (pp. 37-47), Revised Edition.
[2]	P. V. Ammirato. The regulation of somatic embryo development in plant cell cultures: suspension culture techniques and hormone requirements. Bio/Technology, Vol. 1, 1983, pp. 68-74.
[3]	G. Maheswaran, and E. G. Williams. Direct secondary somatic embryogenesis from immature sexual embryos of Trifolium repens cultured in vitro. Ann. Bot., 57, 1986, pp. 109-117.
[4]	L. Bonneau, N. Beranger-Novat., and J. Monin. Somatic embryogenesis and plant regeneration in a woody species: the European Spindle Tree (Euonymus europaeus L.). Plant Cell Reports, Vol. 13, 1994, :135-138.
[5]	P. Das, S. Samantaray, A. V. Roberts, and G. R.Rout. In vitro somatic embryogenesis of Dalbergia sissoo Roxb. – a multipurpose timber yielding tree. Plant Cell Reports, Vol. 16, 1997, pp. 578-582.
[6]	W. W. Su, W. I. Hwang, S. Y. Kim, and Y. Sagawa. Induction of somatic embryogenesis in Azadirachta indica. Plant Cell, Tissue Organ Culture, Vol.50, 1997, pp. 91-95.
[7]	M. Toribio, C.Fernandez, C.Celestino, M.T.Martinez, M. C. San-Jose, and A.M.Vieitez. Somatic embryogenesis in mature Quercus robur trees. Plant Cell, Tissue Organ Culture, Vol.76, 2004, pp.283-287.
[8]	H. K. Moon, J. A. Kim, S. Y. Park, Y. W. Kim, and H. D. Kang. Somatic embryogenesis and plantlet formation from a rare and endangered tree species, Oplopanax elatas. Jour. Plant Biology, Vol. 49, No. 4, 2006, pp. 320-325.
[9]	M. Singh, and R. Chaturvedi. An efficient protocol for cyclic somatic embryogenesis in neem (Azadirachta indica A.Juss.). Int. Jour. Civil & Environmental Engineering, Vol. 1, 2009, :49-51.
[10]	P. Das. Mass cloning of rose and Mussaenda, popular garden plants via. somatic embryogenesis. Horticultural Science, Vol. 37, 2010, pp. 70-78.
[11]	P. Das. Somatic embryogenesis in four tree legumes. Biotechnology Research International, Vol. 1, 2011, pp. 1-8. Article ID 737636.
[12]	Q. Jiang, and P.M. Gresshoff. Shoot control of hypernodulation and aberrant root formation in the har-1 mutant of Lotus japonicus. Funct. Plant Biol., Vol. 29, 2002, pp. 1371-1376.
[13]	I. R. Searle, A. E. Men, T. S. Laniya, D. M. Buzas, I. Iturbe-Ormaetxe, B. J. Carroll., and P. M. Gresshoff. Long distance signaling in nodulation directed by a ClAVATA 1-like receptor kinase. Science, Vol. 299, 2003, pp. 109-112.
[14]	T. Murashige, and F. Skoog,, A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant, Vol. 15, 1962, pp. 473-497.
[15]	T. P. Pasternak, E. Prinsen, F. Ayaydin, P. Miskolczi, G. Potter, and H.Asard . The role of auxin, pH and stress in the activation of embryogenic cell division in leaf protoplast derived cells of alfalfa. Plant Physiology, Vol.129, 2002, 1807-1819.
[16]	R. L.Mott, Tree. In: B. V. Conger (Ed.), Cloning Agricultural Plants via In Vitro Techniques. CRC Press, Boca Raton, 1981, pp. 217-256.
[17]	B. O. C. Ortiz, M. E. P.Reyes, and E. P. M. Balch. Somatic embryogenesis and plant regeneration in A. farnesiana and A.schaffneri. In Vitro Cell Dev.-Plant, Vol. 36, 2000, pp. 268-272.
[18]	C. Akula, A. Akula, and R. Drew. Somatic embryogenesis in clonal neem, A.indica A.Juss. and analysis for in vitro azadirachtin production. In Vitro Cell Dev. Biol.- Plant, Vol. 39, 2003, pp. 304-310.
[19]	B. N. S. Murthy, and P. K. Saxena, Somatic embryogenesis and plant regeneration of neem (A.indica A.Juss.). Plant Cell Reports, Vol. 17, 1998, pp. 469-475.
[20]	K. Shetty, and B. D. Mckersie. Proline, thioproline, and potassium mediated stimulation of somatic embryogenesis in alfalfa (Medicago sativa L.). Plant Science, Vol. 88, 1993, pp. 185-193.
[21]	S. K. Ghanti, K. G. Sujata, S. Rao, M. Udayakumar, and P. B. Kavi Kishore. Role of enzymes and identification of stage– specific proteins in developing somatic embryos of chickpea (Cicer arietinum L.). In Vitro Cell. Dev Biol., -Plant., Vol. 45, 2009, pp. 667-672.
[22]	L. Radojevic L. Plant regeneration of Aesculus hippocastanum L. (Horse chestnut) through somatic embryogenesis. Jour. Plant Physiol., Vol. 132, 1988, pp. 322-326.