Broad Antibacterial Activity of Bothrops jararaca Venom against Bacterial Clinical Isolates

Lucas Henrique Cendron¹, Charise Dallazen Bertol¹, Daiane Bopp Fuentefria¹, Elza Maria Calegari¹, Eliane Dallegrave², Dinara Jaqueline Moura², Kátia Moura³, Maria da Graça Marques³, Luciana Grazziotin Rossato^1*

¹Instituto de Ciências Biológicas, Curso de Farmácia, Universidade de Passo Fundo, Passo Fundo, Brasil.
²Departamento de Farmacociências da Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Brasil.
³Fundação Estadual de Produção e Pesquisa em Saúde-Centro de Informação Toxicológica do Rio Grande do Sul.

DOI: 10.4236/aim.2014.416127                

Abstract

Purpose: To evaluate the antibacterial activity of Bothrops jararaca venom against bacterial clinical isolates. Methods: Antibacterial activity of Bothrops jararaca venom was evaluated through agar diffusion method against the following bacteria: Acinetobacter baumannii, Oxacillinase-producing Acinetobacter baummanii, extended-spectrum β-lactamase-producing (ESBL) Enterobacter aerogenes, Escherichia coli, Escherichia coli ESBL, Klebsiella pneumoniae, Klebsiella pneumoniae ESBL, Proteus mirabilis, Pseudomonas aeruginosa, metallo β-lactamase-producing Pseudomonas aeruginosa, Staphylococcus aureus, oxacillin resistant Staphylococus aureus (ORSA), Staphylococcus epidermidis, and oxacillin resistant Staphylococus epidermidis. Minimum inhibitory concentration was determined through microdilution plate protocol. Results: The venom presented antibacterial activity against all tested bacteria. More pronounced results were observed to Gram- positive bacteria, especially against ORSA. Conclusion: The present study evidenced the great antibacterial potential of Bothrops jararaca venom showing promising results even with resistant bacterial clinical isolates.

Keywords

Snake Venom, Antibiotic Potential, Toxinology, Bothrops

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Queiroz, S. (2010) Identificção da atividade antimicrobiana no veneno da serpente Bothrops moojeni em bactérias gram negativas. 94 p.
[2]	Tapia, C. (2012) Antifúngicos y resistencia. Revista Chilena de Infectología, 29, 357. http://dx.doi.org/10.4067/S0716-10182012000300020
[3]	Chumpolkulwong, N. (2004) Effects of E. coli Ribosomal Protein S12 Mutations on Cell-Free Protein Synthesis. European Journal of Biochemistry, 271, 1127-1134. http://dx.doi.org/10.1111/j.1432-1033.2004.04016.x
[4]	Chopra, I., Hesse, L. and O’neil, L. (2002) Exploiting Current Understanding of Antibiotic Action for Discovery of New Drugs. Journal of Applied Microbiology, 92, 4S-15S. http://dx.doi.org/10.1046/j.1365-2672.92.5s1.13.x
[5]	Ciscotto, P. (2005) Purificação e caracterização biolóGica (estrutural, antibacteriana, antiparasitária, hemolítica e antigênica) de componentes do veneno da serpente Bothrops jararaca. 88 p.
[6]	Ferreira, B.L., Santos, D.O., Dos Santos, A.L., et al. (2011) Comparative Analysis of Viperidae Venoms Antibacterial Profile: A Short Communication for Proteomics. Evidence-Based Complementary and Alternative Medicine, 2011, Article ID: 960267.
[7]	Gomes, V.M., Carvalho, A.O., Da Cunha, M., et al. (2005) Purification and Characterization of a Novel Peptide with Antifungal Activity from Bothrops jararaca Venom. Toxicon, 45, 817-827. http://dx.doi.org/10.1016/j.toxicon.2004.12.011
[8]	Castro, F. (2011) Avaliação da atividade não citotóxica do veneno da cobra Bothrops pauloensis em células mononucleares do sangue periférico humano. 67 p.
[9]	Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72, 248-254. http://dx.doi.org/10.1016/0003-2697(76)90527-3
[10]	CLSI (2009) Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard. 1-76.
[11]	CLSI (2009) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard. 1-88.
[12]	Neves, M. (2008) Avaliação da atividade antifúngica in vitro do veneno das serpentes amazônicas Bothrops atrox (LINNAEUS, 1758) e crotalus durissus ruruima (HOGE, 1965). UEA.
[13]	Cunha, E., Martins, O. (2012) Principais compostos químicos presente nos venenos de cobras dos gêneros Bothrops e Crotalus—uma revisão. Revista Eletrônica de Educação e Ciência, 2, 21-26.
[14]	Caproni, P. (2009) Ação da Bothropstoxina-1 e do veneno total de Bothrops jararacussu irradiados sobre o sistema imune. 60 p.
[15]	Ferreira, B. (2007) Identificação da atividade antibiótica e relação Estrutura-atividade de moléculas de origem sintética e animal. 110 p.
[16]	Du, X.Y. and Clemetson, K.J. (2002) Snake Venom L-Amino Acid Oxidases. Toxicon, 40, 659-665. http://dx.doi.org/10.1016/S0041-0101(02)00102-2
[17]	Faust, A., Niefind, K., Hummel, W. and Schomburg, D. (2007) The Structure of a Bacterial L-Amino Acid Oxidase from Rhodococcus opacus Gives New Evidence for the Hydride Mechanism for Dehydrogenation. Journal of Molecular Biology, 367, 234-248.
[18]	Kommoju, P., Macheroux, P. and Ghisla, S. (2007) Molecular Cloning, Expression and Purification of L-amino Acid Oxidase from the Malayan Pit Viper Calloselasma rhodostoma. Protein Expression and Purification, 52, 89-95. http://dx.doi.org/10.1016/j.pep.2006.09.016
[19]	Braga, M., Martins, A., Amora, D., et al. (2008) Purification and Biological Effects of L-Amino Acid Oxidase Isolated from Bothrops insularis Venom. Toxicon, 51, 199-207. http://dx.doi.org/10.1016/j.toxicon.2007.09.003