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Alleviation of Adverse Effects of Salt Stress in Wheat Cultivars by Foliar Treatment with Antioxidant 2—Changes in Some Biochemical Aspects, Lipid Peroxidation, Antioxidant Enzymes and Amino Acid Contents

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DOI: 10.4236/as.2014.513135    4,723 Downloads   5,641 Views   Citations


Ascobin (compound composed of ascorbic acid and citric acid) is considered one of exogenous protectants which may alleviate the harmful effects of salinity stress. Pot experiments were performed at the screen greenhouse of National Research Centre, Cairo, Egypt to study the effect of foliar treatment of two cultivars of wheat plant with different concentrations of ascobin (0, 200, 400 and 600 mg/l) on some biochemical parameters, antioxidant enzymes, element contents and amino acid constituents of two cultivars of wheat plant grown under different salinity levels (0.0, 3000 and 6000 mg/l) in 2011/2012 and 2012/2013. Salinity with different concentrations levels increased phenolic compounds contents of the two wheat cultivars. The activities of antioxidant enzymes (SOD, CAT, POD, PPO, AXP and GR) dramatically increased due to salinity stress. Amino acid content was increased in cultivar Sids 1, while the content was decreased in cultivar Giza 168 in all salinity treatments. Increments in the above mentioned parameters compared to the untreated plants at normal and stressed conditions. The magnitude of increments was much more pronounced in response to 600 mg/l of ascobin. It could be concluded that, foliar treatment of wheat cultivars with ascobin could partially alleviate the harmful effect of salinity especially at the lower levels of salinity of the two cultivars of wheat at most of the studied parameters.

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The authors declare no conflicts of interest.

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Elhamid, E. , Sadak, M. and Tawfik, M. (2014) Alleviation of Adverse Effects of Salt Stress in Wheat Cultivars by Foliar Treatment with Antioxidant 2—Changes in Some Biochemical Aspects, Lipid Peroxidation, Antioxidant Enzymes and Amino Acid Contents. Agricultural Sciences, 5, 1269-1280. doi: 10.4236/as.2014.513135.


[1] Boyer, J.S. (1982) Plant Productivity and Environment. Science, 218, 443-448.
[2] Gehlot, H.S., Purohit, A. and Shkhawat, N.S. (2005) Metabolic Changes and Protein Patterns Associated with Adaptation to Salinity in Sesamun Indicumcultivars. Journal of Cell and Molecular Biology, 4, 31-39.
[3] Jaleel, C.A., Kishorekumar, B., Manivannan, A., Sankar, B., Gomathinayagam, M. and Panneerselvam, R. (2008) Salt Stress Mitigation by Calcium Chloride in Phyllanthus amarus. Acta Botanica Croatica, 67, 53-62.
[4] Asada, K. (2000) The Water-Water Cycle as Alternative Photon and Electron Sinks. Philosophical Transactions of the Royal Society B, 355, 1419-1431.
[5] Azooz, M.M., Ismail, A.M. and Abou-Elhamed, M.F. (2009) Growth, Lipid Peroxidation and Antioxidant Enzyme Activities as a Selection Criterion for the Salt Tolerance of Three Maize Cultivars Grown under Salinity Stress. International Journal of Agriculture and Biology, 11, 21-26.
[6] Yildirim, B., Yaser, F., Ozpay, T., Ozpay, D.T., Turkozu, D., Terziodlu O. and Tamkoc, A. (2008) Variations in Response to Salt Stress among Field Pea Genotypes (Pisum sativum sp. arvense L.). Journal of Animal and Veterinary Advances, 7, 907-910.
[7] Zhang, S. and Klessing, D.F. (1997) Salicylic Acid Activities a 48-KOMAP Kinase in Tobacco. Plant Cell, 9, 409-424.
[8] Givan, G.V. (1979) Metabolic Detoxification of Ammonia in Tissues of Higher Plants. Phytochemistry, 18, 375-382.
[9] Buettner G.R. and Schafer, F.Q. (2004) Ascorbate as an Antioxidant in Vitamin C. In: Asard, H., May, J.M. and Smirnoff, N., Eds., Functions and Biochemistry in Animals and Plants, Bios Scientific Publishers, Oxford, 173-188.
[10] Chen, Z. and Gallie, D.R. (2004) The Ascorbic acid Redox State Controls Guard Cell Signaling and Stomatal Movement. Plant Cell, 16, 1143-1162.
[11] Wills, R., Lee, T., Graham, D., Mc-Glasson, W. and Hall, H. (1981) Postharvest: An Introduction to the Physiology and Handling of Fruit and Vegetables. CAB International, Wallingford.
[12] Stroganov, B.P. (1962) Physiological Basis of the Salt Tolerance of Plants (under Different Types of Soil Salinization). Izd-vo Akademii nauk SSSR, Moscow.
[13] Larsen, P. Harbo, A., Klungron, S. and Ashein, T.A. (1962) On the Biogenesis of Some Indole Compounds in Acetobacter xylinum. Physiologia Plantarum, 15, 552-565.
[14] Danil, A.D. and George, C.M. (1972) Peach Seed Dormancy in Relation to Endogenous Inhibitors and Applied Growth Substances. Journal of the American Society for Horticultural Science, 17, 621-624.
[15] Stewart, R.C. and Bewley, J.D. (1980) Lipid Peroxidation Associated with Accelerated Aging of Soybean Axes. Plant Physiology, 65, 245-248.
[16] Chapman, H.D. and Pratt, P.F. (1978) Methods of Analysis for Soils, Plant and Water. University of California Division of Agricultural Science, Berkeley.
[17] MuKherjee, S.P. and Choudhuri, M.A. (1983) Implication of Water Stress—Induced Changes in the Levels of Endogenous Ascorbic Acid and Hydrogen Peroxide in Vigna Seedling. Physiologia Plantarum, 58, 166-170.
[18] Dhindsa, R., Plumb-Dhindsa, P. and Thorpe, T. (1981) Leaf Senescence Correlated Permeability, Lipid Peroxidation and Decreased Levels of Superoxide Dismutase and Catalase. Journal of Experimental Botany, 32, 93-101.
[19] Chen, Y., Cao, X.D., Lu, Y. and Wang, X.R. (2000) Effect of Rare Earth Metal Ions and Their EDTA Complexes on Antioxidant Enzymes of Fish Liver. Bulletin of Environmental Contamination and Toxicology, 65, 357-365.
[20] Bergmeyer, H.U. (1974) Methods of Enzymatic Analysis I. 2nd Edition, Academic Press, New York.
[21] Kar, M. and Mishra, D. (1976) Catalase, Peroxidase and Polyphenol Oxidase Activities during Rice Leaf Senescence. Plant Physiology, 57, 315-319.
[22] Chen, G. and Asada, K. (1992) Inactivation of Ascorbate Peroxidase by Thoils Requires Hydrogen Peroxide. Plant and Cell Physiology, 33, 117-123.
[23] Foyer, C.H. and Halliwell, B. (1976) The Presence of Glutathione and Glutathione Reductase in Chloroplasts: A Proposed Role in Ascorbic Acid Metabolism. Planta, 133, 21-25.
[24] MSTAT-C (1988) MSTAT-C, a Microcomputer Program for the Design, Arrangement, and Analysis of Agronomic Research. Michigan State University East Lansing, East Lansing.
[25] Rice-Evans, C., Miller, N.J. and Paganga, G. (1997) Antioxidant Properties of Phenolic Compounds. Trends in Plant Science, 2, 152-159.
[26] Parida, A. and Das, A.B. (2005) Salt Tolerance and Salinity Effects on Plants: A Review Original Research Article. Ecotoxicology and Environmental Safety, 60, 324-349.
[27] Radi, A.A., Farghaly, F.A. and Hamada, A.M. (2013) Physiological and Biochemical Responses of Salt-Tolerant and Salt-Sensitive Wheat and Bean Cultivars to Salinity. Journal of Biology and Earth Sciences, 3, 72-88.
[28] El-Beltagi, H.S., Salama, Z.A. and El Hariri, D.M. (2008) Some Biochemical Markers for Evaluation of Flax Cultivars under Salt Stress Conditions. Journal of Natural Fibers, 5, 316-330.
[29] Sadak, M.S., Abdelhamid, M.T. and El-Saady, M.A. (2010) Physiological Responses of Faba Bean Plant to Ascorbic Acid Grown under Salinity Stress. Egyptian Journal of Agronomy, 32, 89-106.
[30] Zhang, J. and Kirkham, M.B. (1996) Sorghum and Sunflower Seedlings as Affected by Ascorbic Acid, Benzoic Acid and Prophyl Gallate. Journal of Plant Physiology, 149, 489-493.
[31] Dolatabadian, A. and Jouneghani, R.S. (2009) Impact of Exogenous Ascorbic Acid on Antioxidant Activity and Some Physiological Traits of Common Bean Subjected to Salinity Stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37, 165-172.
[32] Ebrahimian, E. and Bybordi, A. (2012) Effect of Salinity, Salicylic Acid, Silicium and Ascorbic Acid on Lipid Peroxidation, Antioxidant Enzyme Activity and Fatty Acid Content of Sunflower. African Journal of Agricultural Research, 7, 3685-3694.
[33] Bahari, A., Pirdashti, H. and Yaghubi, M. (2013) The Effects of Amino Acid Fertilizers Spraying on Photosynthetic Pigments and Antioxidant Enzymes of Wheat (Triticum aestivum L.) under Salinity Stress. International Journal of Agronomy and Plant Production, 4, 787-793.
[34] Zhang, L., Zhang, G., Wang, Y., Zhou, Z., Meng, Y. and Chen, B. (2013) Effect of Soil Salinity on Physiological Characteristics of Functional Leaves of Cotton Plants. Journal of Plant Research, 126, 293-304.
[35] Bowler, C., Montagu, M.V. and Inze, D. (1992) Superoxide Dismutase and Salt Stress Tolerance. Annual Review of Plant Biology, 43, 83-116.
[36] Vaidyanathan, H., Sivakumar, P., Chakrabarsty, R. and Thomas, G. (2003) Scavenging of Reactive Oxygen Species in NaCl-Stressed Rice (Oryza sativa L.) Differential Response in Salt-Tolerant and Sensitive Varieties. Plant Science, 165, 1411-1418.
[37] Asada, K. (2006) Production and Scavenging of Reactive Oxygen Species in Chloroplasts and Their Functions. Plant Physiology, 141, 391-396.
[38] Mandhania, S., Madan, S. and Sawhney, V. (2006) Antioxidant Defense Mechanism under Salt Stress in Wheat Seedlings. Plant Biology, 50, 227-231.
[39] Kodandaramaiah, J. (1983) Physiological Studies on the Influence of B-Vitamins on Leaf and Fruit Metabolism in Cluster Beans Cyamopsis tetagonoloba L. Tanb. Ph.D. Thesis, Sri Venkateswara University, Tirupati.
[40] Asada, K. (1999) The Water-Water Cycle in Chloroplasts: Scavenging of Active Oxygens and Dissipation of Excess photons. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 601-639.
[41] Padh, H. (1990) Cellular Function of Ascorbic Acid. Biochemistry and Cell Biology, 68, 1166-1173.
[42] Shalata, A. and Neumann, P.M. (2001) Exogenous Ascorbic Acid (Vitamin C) Increases Resistance to Stress and Reduces Lipid Peroxidation. Journal of Experimental Botany, 52, 2207-2221.
[43] Ashraf, M. and Harris, P.J.C. (2004) Potential Biochemical Indicators of Salinity Tolerance in Plants. Plant Science, 166, 3-16.
[44] Tammam, A.A., Alhamd, M.F.A. and Hemeda, M.M. (2008) Study of Salt Tolerance in Wheat (Triticum aestium L.) Cultivar Banysoif 1. Australian Journal of Crop Science, 1, 115-125.
[45] Kovács, Z., Simon-Sarkadi, L., Vashegyi, I. and Kocsy, G. (2012) Different Accumulation of Free Amino Acids during Short and Long-Term Osmotic Stress in Wheat. The Scientific World Journal, 2012, Article ID: 216521.
[46] Keutgen, A.J. and Pawelzik, E. (2008) Contribution of Amino Acids to Strawberry Fruit Quality and Their Relevance as Stress Indicators under NaCl Salinity. Food Chemistry, 111, 642-647.
[47] Verbruggen, N. and Hermans, C. (2008) Proline Accumulation in Plants: A Review. Amino Acids, 35, 753-759.
[48] Slocum, R.D. and Weinstein, K.H. (1990) Stress-Induced Putrescine Accumulation as a Mechanism of Ammonia Detoxification in Cereal Leaves. In: Flores, H.E., Ed., Polyamines and Ethylene: Biochemistry, Physiology and Interaction, American Society of Plant Biologists, Rockville, 157-167.
[49] Hozayn, M., Abd El-Monem, A.A., Abd El-hamidEbtihal, E.M. and Abdul Qados, A.M.S. (2013) Amelioration of Salinity Stress in Mungbean (Vigna radiate L). Plant by Soaking in Arginine. Journal of Applied Sciences Research, 9, 393-401.
[50] Sadak, M.S. and Dawood, M.G. (2014) Role of Ascorbic Acid and α Tocopherol in Alleviating Salinity Stress on Flax Plant (Linum usitatissimum L.). Journal of Stress Physiology & Biochemistry, 10, 93-111.
[51] Hanafy, A.H. (1996) Physiological Studies on Tiploun and Nitrate Accumulation in Lettuce Plants. Mansoura University Journal of Agricultural Sciences, 21, 3971-3994.
[52] Mohamedin, A.A.M., Abd El-Kader, A.A. and Nadia, M.B. (2006) Response of Sunflower (Helianthus annuus L.) to Plants Salt Stress under Different Water Table Depths. Journal of Applied Sciences Research, 2, 1175-1184.
[53] Gemea, I., Navarro, J., Moral, R., Iborra, M., Palacios, G. and Mataix, J. (1996) Salinity and Nitrogen Fertilization Affecting the Macronutrient Content and Yield of Sweet Pepper Plants. Journal of Plant Nutrition, 19, 353-359.
[54] Sheteaw, S.A. (2007) Improving Growth and Yield of Salt-Stressed Soybean by Exogenous Application of Jasmonic Acid and Ascobin. International Journal of Agriculture and Biology, 9, 473-478.

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