Share This Article:

Adherence of a Clinical Strain of Mycoplasma fermentans to Human Cultured Epithelial Cells

Full-Text HTML XML Download Download as PDF (Size:4980KB) PP. 726-735
DOI: 10.4236/aim.2014.411079    2,724 Downloads   3,084 Views   Citations

ABSTRACT

This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and genitourinary tract infections. We investigated the presence of putative adhesins from strain P-140. A surface protein of 47 kDa, labeled SP47, isolated from M. fermentans P-140, seems to be involved in acterial adherence on HEp-2 cells; this study could allow helping understand the interaction between mycoplasmas and their hosts. Anti-SP47 antibodies inhibited the formation of bacterial clusters and adherence to cultured cells, quantified by ELISA. M. fermentans P-140 was more efficient at adhering to cultured HEp-2 cells than PG-18.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Rosales-Pérez, M. , Giono-Cerezo, S. , Girón, J. , Yáñez, A. and Cedillo, L. (2014) Adherence of a Clinical Strain of Mycoplasma fermentans to Human Cultured Epithelial Cells. Advances in Microbiology, 4, 726-735. doi: 10.4236/aim.2014.411079.

References

[1] Rotten, S. and Yehudith, N. (1998) Subversion and Exploitation of Host Cellsby Mycoplasmas. IEEE Transactions on Instrumentation and Measurement, 6, 436-441.
[2] Kostyal, D.A., Butler G.H. and Beezhold, D.H. (1994) A 48 Kilodalton Mycoplasma fermentans Membrane Protein Induces Cytokine Secretion by Humanmonocytes. Infection and Immunity, 62, 3793-3800.
[3] Rawadi, G. and Roman-Román, S. (1996) Mycoplasma Membrane Lipoproteins Induce Proinflammato-
ry Cytokines by a Mechanism Cytokines by a Mechanism Distinct from That of Lipopolysaccharide. Infection and Immunity, 64, 637-643.
[4] Razin, S. and Jacobs, E. (1992). Mycoplasma Adhesion. Journal General Microbiology, 138, 407-422. http://dx.doi.org/10.1099/00221287-138-3-407
[5] Cassell, G.H. and Cole, B.C. (1981) Mycoplasmas as Agents of Human Disease. New England Journal of Medicine, 304, 80-89. http://dx.doi.org/10.1056/NEJM198101083040204
[6] Baseman, J.B. and Tully, J.G. (1997) Mycoplasmas: Sophisticated, Reemerging, Andburdened by Their Notoriety. Emerging Infectious Diseases, 3, 21-32. http://dx.doi.org/10.3201/eid0301.970103
[7] Ueno, P.M., Timenetsky, J., Centonze, V.E., Wewer, J.J., Cagle, M., Stein, M.A., Krishnan, M. and Baseman, J.B. (2008) Interaction of Mycoplasma genitalium with Host Cells: Evidence for Nuclear Localization. Microbiology, 154, 3033-3041. http://dx.doi.org/10.1099/mic.0.2008/020735-0
[8] Krause, D.C. (1998) Mycoplasma pneumoniae Cytadherence: Organization Andassembly of the Attachment Organelle. IEEE Transactions on Instrumentation and Measurement, 6, 15-19.
[9] Krause, D.C. and Baseman, J.B. (1982) Mycoplasma pneumoniae Proteins That Selectively Bind to Host Cells. Infection and Immunity, 37, 382-386.
[10] Zhang, Q. and Wise K. (1996) Molecular Basis of Size and Antigenic Variation of a Mycoplasma hominis Adhesin Encoded by Divergent vaa Genes. Infection and Immunity, 64, 2737-2744.
[11] Girón, J.A., Lange, M. and Baseman, J.B. (1996) Adherence, Fibronectin Binding, and Induction of Cytoskeleton Reorganization in Cultured Human Cells by Mycoplasma penetrans. Infection and Immunity, 64, 197-208.
[12] Saada, A.B., Terespolski, Y., Adoni, A. and Kahane, I. (1991) Adherence of Ureaplasma urealyticum to Human Erythrocytes. Infection and Immunity, 59, 467-469.
[13] Wise, K.S., Kim, H.F., Theiss, P.M. and Lo, S.C. (1993) A Family of Strain-Variant Surface Lipoproteins of Mycoplasma fermentans. Infection and Immunity, 61, 3327-3333.
[14] Wise, K.S., Foecking, M.F., Roske, K., Lee, Y.J., Lee, Y.M., Madan, A. and Calcutt, M.J. (2006) Distinctive Repertoire of Contingency Genes Conferring Mutation-Based Phase Variation and Combinatorial Expression of Surface Lipoproteins in Mycoplasma capricolum subsp. Capricolum of the Mycoplasma mycoides Phylogenetic Cluster. Journal of Bacteriology, 188, 4926-4941.
http://dx.doi.org/10.1128/JB.00252-06
[15] Stadtlander, C.T., Zuhua, C., Watson, H.L. and Cassell, G.H. (1991) Protein and Antigen Heterogeneity among Strains of Mycoplasma fermentans. Infection and Immunity, 59, 3319-3322.
[16] Theiss, P.M., Kim, M.F. and Wise, K.S. (1993) Differential Protein Expression and Surface Presentation Generates High-Frequency Antigenic Variation in Mycoplasma fermentans. Infection and Immunity, 61, 5123-5128.
[17] Horowitz, S., Evison, B., Borer, A. and Horowitz, J. (2000) Mycoplasma fermentans in Rheumatoid Arthritis and Other Inflammatory Arthritides. Journal of Rheumatology, 27, 2747-2753.
[18] Haier, J., Nasralla, A., Franco, R. and Nicolson, G.L. (1999) Detection of Mycoplasmal Infections in Blood of Patients with Rheumatoid Arthritis. Rheumatology, 38, 504-509.
http://dx.doi.org/10.1093/rheumatology/38.6.504
[19] Johnson, S., Sidebottom, D., Bruckner, F. and Collins, D. (2000) Identification of M. fermentans in Synovial Fluid Samples from Arthritis Patients with Inflammatory Disease. Journal of Clinical Microbiology, 38, 90-93.
[20] Rivera, A., Yánez, A., León-Tello, G., Gil, C., Giono, S., Barba, E. and Cedillo, L. (2002) Experimental Arthritis Induced by a Clinical Mycoplasma fermentans Isolate. BMC Musculoskeletal Disorders, 3, 15-21. http://dx.doi.org/10.1186/1471-2474-3-15
[21] Yánez, A., Martínez-Ramos, A., Calixto, T., González-Matus, F.J., Rivera-Tapia, J.A., Giono, S., Gil, C. and Cedillo, L. (2013) Animal Model of Mycoplasma fermentans Respiratory Infection. BMC Research Notes, 6, 9. http://www.biomedcentral.com/1756-0500/6/9 http://dx.doi.org/10.1186/1756-0500-6-9
[22] Kannan, T.R., Provenzano, D., Wright, J.R. and Baseman, J.B. (2005) Identification and Characterization of Human Surfactant Protein A Binding Protein of Mycoplasma pneumoniae. Infection and Immunity, 73, 2828-2834. http://dx.doi.org/10.1128/IAI.73.5.2828-2834.2005
[23] Harlow, E. and Lane, D. (1988) Immunobloting and Immunoassay. In: Harlow, E. and Lane, D., Eds., Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, 471-553.
[24] Laemmli, U.K. (1970) Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685. http://dx.doi.org/10.1038/227680a0
[25] Baseman, J.B., Reddy, S.P. and Dallo, S.F. (1996) Interplay between Mycoplasma Surface Proteins, Airway Cells, and the Protean Manifestations of Mycoplasma-Mediated Human Infections. American Journal of Respiratory Critical Care Medicine, 154, S137-S144.
http://dx.doi.org/10.1164/ajrccm/154.4_Pt_2.S137
[26] Layh-Schmitt, G., Podtelejnikov, A. and Mann, M. (2000) Proteins Complexed to the P1 Adhesin of Mycoplasma pneumoniae. Microbiology, 146, 741-747.
[27] Zhang, Q., Young, T.E. and Ross, R.E. (1995) Identification and Characterization of a Mycoplasma hyopneummoniae Adhesin. Infection and Immunity, 63, 1013-1019.
[28] Chambaud, I., Wróblewski, H. and Blanchard, A. (1992) Interactions between Mycoplasma Lipoproteins and the Host Immune System. IEEE Transactions on Instrumentation and Measurement, 7, 493-499.
[29] Bordier, C. (1981) Phase Separation of Integral Membrane Proteins in Triton X-114 Solution. Journal of Biological Chemistry, 256, 1604-1607.
[30] Mühlradt, P.F. and Schade, U. (1991) MDHM, a Macrophage-Stimulatory Product of Mycoplasma fermentans, Leads to in Vitro Interleukin-1 (IL-1), IL-6, Tumor Necrosis Factor, and Prostaglandin Production and Is Pyrogenic in Rabbits. Infection and Immunity, 59, 3969-3974.
[31] Quentmeier, H., Schmitt, E., Kirchhoff, H., Grote, W. and Mühlradt, P.F. (1990) Mycoplasma fermentans-Derived High Molecular-Weight Material Induces Interleukin-6 Release in Cultures of Murine Macrophages and Human Monocytes. Infection and Immunity, 58, 1273-1280.
[32] Girón, J.A., Torres, A.G., Freer, E. and Kaper, J.B. (2002) The Flagella of Enteropathogenic Escherichia coli Mediate Adherence to Epithelial Cells. Molecular Microbiology, 44, 361-379.
http://dx.doi.org/10.1046/j.1365-2958.2002.02899.x
[33] Leigh, S.A. and Wise, K.S. (2002) Identification and Functional Mapping of the Mycoplasma fermentans P29 Adhesin. Infection and Immunity, 70, 4925-4935.
http://dx.doi.org/10.1128/IAI.70.9.4925-4935.2002
[34] Beyers, T.M., Lai, W.C., Read, R.W. and Pakes, S.P. (1994) Mycoplasma pulmonis 46-KDa Trypsin-Resistant Protein Adheres to Rat Tracheal Epitelial Cells. Laboratory Animal Science, 44, 573-578.
[35] Citti, C., Watson-McKown, R., Droesse, M. and Wise, K.S. (2000) Gene Families Encoding Phase- and Size-Variable Surface Lipoproteins of Mycoplasma hyorhinis. Journal of Bacteriology, 182, 1356-1363. http://dx.doi.org/10.1128/JB.182.5.1356-1363.2000

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.