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Probing the Cell Cycle with Flow Cytometry

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DOI: 10.4236/jbise.2014.79069    3,743 Downloads   4,630 Views   Citations


Flow cytometry is a versatile technique to study different aspects of the cell cycle from subpopulations of cells to detailed cell kinetic information. In this paper a basic review of cell kinetic parameters is presented followed by detailed descriptions of the different flow cytometric methodologies that can be used to extract pertinent information for a particular study. The methodologies range from simple DNA profile analysis, the use of bromodeoxyuridine to cell cycle-associated proteins such as the cyclins.

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The authors declare no conflicts of interest.

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Wilson, G. (2014) Probing the Cell Cycle with Flow Cytometry. Journal of Biomedical Science and Engineering, 7, 698-711. doi: 10.4236/jbise.2014.79069.


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