Share This Article:

Suppression of Methane Gas Emissions and Analysis of the Electrode Microbial Community in a Sediment-Based Bio-Electrochemical System

Abstract Full-Text HTML XML Download Download as PDF (Size:684KB) PP. 252-266
DOI: 10.4236/aim.2014.45032    3,559 Downloads   5,103 Views   Citations

ABSTRACT

The effects of bioelectrochemical systems (BESs) for the suppression of methane gas emissions from sediment were examined using a laboratory-scale reactor system. Methane gas emissions from acetate were suppressed by approximately 36% from control based on the installation of a BES in which carbon-graphite electrodes were buried in sediment and arbitrarily set at certain oxidative potentials (+300 mV vs Ag/AgCl) using a potentiostat. Meanwhile, methane gas emissions increased in the BES reactor where the electrode potential was set at -200 mV. Results obtained from pyrotag sequencing analysis of the microbial community on the surface of the buried electrodes targeting 16S rRNA genes demonstrated that the genusGeobacterhad drastically propagated in a sample from the reactor where the electrodes were buried. Quantitative analysis of 16S rRNA genes of archaea also revealed that the archaeal population had decreased to approximately 1/6 of its original level on the electrode of the BES set at +300 mV. This implied that the oxidation-reduction potential (ORP) in the sediment was raised to the inhibition level for methanogenesis in the vicinity of the buried electrode. Analysis of electron flux in the experiment revealed that electrons intrinsically used for methanogenesis were recovered via current generation in the sediment where a potential of +300 mV was set for the electrode, although most electrons donated from acetate were captured by oxygen respiration and other electron-accepting reactions. These results imply that BES technology is suitable for use as a tool for controlling re-dox-dependent reactions in natural environments, and that it also brought about changes in the microbial population structure and methanogenic activity in sediment.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Ueno, Y. and Kitajima, Y. (2014) Suppression of Methane Gas Emissions and Analysis of the Electrode Microbial Community in a Sediment-Based Bio-Electrochemical System. Advances in Microbiology, 4, 252-266. doi: 10.4236/aim.2014.45032.

References

[1] Rabaey, K. and Rozendal, R.A. (2010) Microbial Electrosynthesis: Revisiting the Electrical Route for Bioproduction. Nature Reviews Microbiology, 8, 706-716. http://dx.doi.org/10.1038/nrmicro2422
[2] De Schamphelaire, L., Rabaey, K., Boeckx, P., Boon, N. and Verstraete, W. (2008) Outlook for Benefits of Sediment Microbial Fuel Cells with Two Bio-Electrodes. Microbial Biotechnology, 1, 446-462.http://dx.doi.org/10.1111/j.1751-7915.2008.00042.x
[3] IPCC (2007) Climate changes.Synthesis Report.http://www.ipcc.ch/pdf/assessment report/ar4/ syr/ar4_syr_spm.pdf
[4] Le Mer, J. and Roger, P. (2001) Production, Oxidation, Emission and Consumption of Methane by Soils: A Review. European Journal of Soil Biology, 37, 25-50. http://dx.doi.org/10.1016/S1164-5563(01)01067-6
[5] Lovley, D.R. (2006) Bug Juice: Harvesting Electricity with Microorganisms. Nature Reviews, 4, 497-508.
[6] Devai, I. and Delaune, R.D. (1995) Evidence for Phosphine Production and Emission from Louisiana and Florida Marsh Soils. Organic Geochemistry, 23, 277-279. http://dx.doi.org/10.1016/0146-6380(95)00021-6
[7] Singh, S.N. (2001) Exploring Correlation between Redox Potential and Other Edaphic Factors in Field and Laboratory Conditions in Relation to Methane Efflux. Environment International, 4, 265-274.http://dx.doi.org/10.1016/S0160-4120(01)00055-1
[8] Stumm, W. and Morgan, J.J. (1996) Aquatic Chemistry, Chemical Equilibria and Rates in Natural Waters. 3rd Edition, John Wiley & Sons, Inc., New York.
[9] Ishii, S., Hotta, Y. and Watanabe, K. (2008) Methanogenesis versus Electrogenesis: Morphological and Phylogenetic Comparisons of Microbial Communities. Bioscience, Biotechnology, and Biochemistry, 72, 286-294.http://dx.doi.org/10.1271/bbb.70179
[10] Ueno, Y. and Kitajima, Y. (2012) Suppression of Greenhouse Gas Emissions from Sediment by Bio Electrochemical System. Environmental Engineering and Management Journal, 11, 1833-1837.
[11] Tatara, M., Yamazawa, A., Ueno, Y., Fukui, H., Goto, M. and Sode, K. (2005) High-Rate Thermophilic Methane Fermentation on Short-Chain Fatty Acids in a Down-Flow Anaerobic Packed-Bed Reactor. Bioprocess and Biosystems Engineering, 27, 105-113. http://dx.doi.org/10.1007/s00449-004-0387-8
[12] Logan, B.E., Hamelers, B., Rozendal, R., Schröder, U., Keller, J. and Freguia, S. (2006) Microbial Fuel Cells: Methodology and Technology. Environmental Science & Technology, 40, 5181-5192. http://dx.doi.org/10.1021/es0605016.
[13] Caporaso, J.G., Lauber, C.L., Walters, W.A., Berg-Lyons, D. and Lozupone, C.A. (2011) Global Patterns of 16S rRNA Diversity at a Depth of Millions of Sequences Per Sample. Proceedings of the National Academy of Sciences of the United States of America, 108, 4516-4522.
http://dx.doi.org/10.1073/pnas.1000080107
[14] Dowd, S.E., Callaway, T.R., Wolcott, R.D., Sun, Y. and McKeehan, T. (2008) Evaluation of the Bacterial Diversity in the Feces of Cattle Using 16S rDNA Bacterial Tag-Encoded FLX Ampliconpyro sequencing (bTEFAP). BMC Microbiology, 8, 125. http://dx.doi.org/10.1186/1471-2180-8-125
[15] Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J. and Zhang, Z. (1997) Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs. Nucleic Acids Research, 25, 3389-3402.http://dx.doi.org/10.1186/1471-2180-8-125.
[16] Wang, Q., Garrity, G.M., Tiedje, J.M. and Cole, J.R. (2007) Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy. Applied and Environmental Microbiology, 73, 5261-5267.http://dx.doi.org/10.1128/AEM.00062-07
[17] Sun, Y., Cai, Y., Liu, L., Yu, F. and Farrell, M.L (2009) ESPRIT: Estimating Species Richness Using Large Collections of 16S rRNA Pyrosequences. Nucleic Acids Research, 37, e76.
http://dx.doi.org/10.1093/nar/gkp285
[18] Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan,P.A.,McWilliam,H.,Valentin, F., Wallace,I.M.,Wilm,A.,Lopez,R.,Thompson,J.D., Gibson,T.J. and Higgins, D.G. (2007) Clustal W and Clustal X Version 2.0. Bioinformatics,23,2947-2948. http://dx.doi.org/10.1093/bioinformatics/btm404
[19] Saitou, N. and Nei, M. (1987) The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees. Molecular Biology and Evolution, 4, 406-425.
[20] Holmes, D.E., Finneran, K.T., O’Neil, R.A. and Lovley, D.R. (2002) Enrichment of Members of the Family Geobacteraceae Associated with Stimulation of Dissimilatory Metal Reduction in Uranium-Contaminated Aquifer Sediments. Applied and Environmental Microbiology, 68, 2300-2306. http://dx.doi.org/10.1128/AEM.68.5.2300-2306.2002
[21] Lueders, T. and Friedrich, M.W. (2003) Evaluation of PCR Amplification Bias by Terminal Restriction Fragment Length Polymorphism Analysis of Small-Subunit rRNA and mcrA Genes by Using Defined Template Mixtures of Methanogenic Pure Cultures and Soil DNA Extracts. Applied and Environmental Microbiology, 69, 320-326. http://dx.doi.org/10.1128/AEM.69.1.320-326.2003
[22] Yamada, T., Imachi, H., Ohashi, A., Hrada, H., Harada, S., Kamagata, Y. and Sekiguchi, Y., (2007) Bellilinea caldifistulaegen. nov., sp. nov. and Longilinea arvoryzae gen. nov., sp. nov., Strictly Anaerobic, Filamentous Bacteria of the Phylum Chloroflexi Isolated from Methanogenic Propionate Degrading Consortia. International Journal of Systematic and Evolutionary Microbiology, 57, 2299-2306. http://dx.doi.org/10.1099/ijs.0.65098-0
[23] Davey, M.E., Wood, W.A., Key, R., Nakamura, K. and Stahl, D. (1993) Isolation of Three Species of Geotoga and Petrotoga: Two New Genera, Representing a New Lineage in the Bacterial Line of Descent Distantly Related to the “Thermotogales”. Systematic and Applied Microbiology, 16, 191-200. http://dx.doi.org/10.1016/S0723-2020(11)80467-4
[24] Bond, D.R. and Lovley, D.R. (2003) Electricity Production by Geobacter sulfurreducens Attached to Electrodes. Applied and Environmental Microbiology, 69, 1548-1555. http://dx.doi.org/10.1128/ AEM.69.3.1548-1555.2003
[25] Yates, M.D., Kiely, P.D., Call, D.F., Rismani-Yazdi, H. and Bibby, K. (2012) Convergent Development of Anodic Bacterial Communities in Microbial Fuel Cells. The ISME Journal, 6, 2002-2013. http://dx.doi.org/10.1038/ismej.2012.42
[26] Lee, J., Phung, N.T., Chang, I.S., Kim, B.H. and Sung, H.C. (2003) Use of Acetate for Enrichment of Electrochemically Active Microorganisms and their 16S rDNA Analyses. FEMS Microbiology Letters, 223, 185-191.http://dx.doi.org/10.1016/S0378-1097(03)00356-2
[27] Kato, S., Kai, F., Nakamura, R., Watanabe, K. and Hashimoto, K. (2010) Respiratory Interactions of Soil Bacteria with (Semi) Conductive Iron-Oxide Minerals. Environmental Microbiology, 12, 3114-3123.http://dx.doi.org/10.1111/j.1462-2920.2010.02284.x
[28] Sasaki, D., Sasaki, K., Watanabe, A., Morita, M., Igarashi, Y. and Ohmura, N. (2013) Efficient Production of Methane from Artificial Garbage Waste by a Cylindrical Bioelectrochemical Reactor Containing Carbon Fiber Textiles. AMB Express, 3, 17. http://dx.doi.org/10.1186/2191-0855-3-17
[29] Cheng, S., Xing, D., Call, D.F. and Logan, B.E. (2009) Direct Biological Conversion of Electrical Current into Methane by Electromethanogenesis. Environmental Science & Technology, 43, 3953-3958. http://dx.doi.org/10.1021/es803531g
[30] Shimoyama, T., Komukai, S., Yamazawa, A., Ueno, Y., Logan, B.E. and Watanabe, K. (2008) Electricity Generation from Model Organic Wastewater in a Cassette-Electrode Microbial Fuel Cell. Applied and Environmental Microbiology, 80, 325-330. http://dx.doi.org/10.1007/s00253-008-1516-0
[31] Ryckelynck, N., Stecher, H. and Reimers, C.E. (2005) Understanding the Anodic Mechanism of a Seafloor Fuel Cell: Interactions between Geochemistry and Microbial Activity. Biogeochemistry, 76, 113-139.http://dx.doi.org/10.1007/s10533-005-2671-3
[32] Conrad, R. (2002) Control of Microbial Methane Production in Wetland Rice Fields. Nutrient Cycling in Agroecosystems, 64, 59-69. http://dx.doi.org/10.1023/A:1021178713988
[33] Rosenbaum, M. (2005) In Situ Electrooxidation of Photobiological Hydrogen in a Photobioelectrochemical Fuel Cell Based on Rhodobacter sphaeroides. Environmental Science & Technology, 39, 6328-6333. http://dx.doi.org/10.1021/es0505447.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.