Immunolocalization of Ephexin-1 in the Developing Canine Cerebellum

DOI: 10.4236/nm.2014.51002   PDF   HTML   XML   3,304 Downloads   4,113 Views  

Abstract

Ephexin-1 functions as guanine nucleotide exchange factors for the Rho-type GTPases which have important roles in neuronal development including axon guidance, migration, morphogenesis, and plasticity of neurons. As little is known about ephexin-1 in the cerebellum, we investigated the immunolocalization of ephexin-1 in the developing canine cerebellum. While the cellular maturation was followed by the temporal pattern, the calbindin D-28k and ephexin-1 immunoreactivities gradually increased in developing canine cerebellum. When compared to the calbindin D-28k immunoreactivities, belated ephexin-1 immunolocalization was observed in the Purkinje cells which aligned a single layer during cerebellar development. These results suggest that ephexin-1 might play an important role in the development of the Purkinje cells during the first two postnatal weeks based on its immunolocalization in the present study.

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Park, H. , Chang, I. , Kim, H. and Yoon, S. (2014) Immunolocalization of Ephexin-1 in the Developing Canine Cerebellum. Neuroscience and Medicine, 5, 9-14. doi: 10.4236/nm.2014.51002.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Roger, J.H. (1989) Immunoreactivity for Calretinin and Other Calcium-Binding Proteins in Cerebellum. Neuroscience, 31, 711-721. http://dx.doi.org/10.1016/0306-4522(89)90435-1
[2] Fortin, M., Marchand, R. and Parent, A. (1998) Calcium-Binding Proteins in Primate Cerebellum. Neuroscience Research, 30, 155-168. http://dx.doi.org/10.1016/S0168-0102(97)00124-7
[3] Siso, S., Tort, S., Aparici, C., Perez, L., Vidal, E. and Pumarola, M. (2003) Abnormal Neuronal Expression of the Calcium-Binding Proteins, Parvalbumin and Calbindin D-28k, in Aged Dogs. Journal of Comparative Pathology, 128, 9-14. http://dx.doi.org/10.1053/jcpa.2002.0597
[4] Celio, M.R. (1990) Calbindin D-28k and Parvalbumin in the Rat Nervous System. Neuroscience, 35, 375-475.
http://dx.doi.org/10.1016/0306-4522(90)90091-H
[5] Bastianelli, E. (2003) Distribution of Calcium-Binding Proteins in the Cerebellum. Cerebellum, 2, 242-262.
http://dx.doi.org/10.1080/14734220310022289
[6] Yoon, S.P., Chung, Y.Y., Chang, I.Y., Kim, J.J., Moon, J.S. and Kim, H.S. (2002) Postnatal Development of Calbindin D-28k and Parvalbumin Immunoreactivities in the Canine Hippocampus. Journal of Chemical Neuroanatomy, 19, 143-154. http://dx.doi.org/10.1016/S0891-0618(00)00059-4
[7] Moon, J.S., Kim, J.J., Chang, I.Y., Chung, Y.Y., Jun, J.Y., You, H.J. and Yoon, S.P. (2002) Postnatal Development of Calbindin D-28k and Parvalbumin Immunoreactivities in the Canine Anterior Cingulated Cortex: Transient Expression in Layer V Pyramidal Cells. International Journal of Developmental Neuroscience, 20, 511-519.
http://dx.doi.org/10.1016/S0736-5748(02)00056-4
[8] Phemister, R.D. and Young, S. (1968) The Postnatal Development of the Canine Cerebellar Cortex. Journal of Comparative Neurology, 134, 243-253. http://dx.doi.org/10.1002/cne.901340209
[9] Ayala, R., Shu, T. and Tsai, L.H. (2007) Trekking across the Brain: The Journey of Neuronal Migration. Cell, 128, 29-43. http://dx.doi.org/10.1016/j.cell.2006.12.021
[10] Peng, Y.J., He, W.Q., Tang, J., Tao, T., Chen, C., Gao, Y.Q., Zhang, W.C., He, X.Y., Dai, Y.Y., Zhu, N.C., Lv, N., Zhang, C.H., Qiao, Y.N., Zhao, L.P., Gao, X. and Zhu, M.S. (2010) Trio Is a Key Guanine Nucleotide Exchange Factor Coordinating Regulation of the Migration and Morphogenesis of Granule Cells in the Developing Cerebellum. The Journal of Biological Chemistry, 285, 24834-24844. http://dx.doi.org/10.1074/jbc.M109.096537
[11] Rodrigues, N.R., Theodosiou, A.M., Nesbit, M.A., Campbell, L., Tandle, A.T., Saranath, D. and Davies, K.E. (2000) Characterization of Ngef, a Novel Member of Dbl Family of Genes Expressed Predominantly in the Caudate Nucleus. Genomics, 65, 53-61. http://dx.doi.org/10.1006/geno.2000.6138
[12] Jaffe, A.B. and Hall, A. (2005) Rho GTPases: Biochemistry and Biology. Annual Review of Cell and Developmental Biology, 21, 247-269. http://dx.doi.org/10.1146/annurev.cellbio.21.020604.150721
[13] Luo, L. (2000) Rho GTPases in Neuronal Morphogenesis. Nature Reviews. Neuroscience, 1, 173-180.
http://dx.doi.org/10.1038/35044547
[14] Wong, K., Ren, X.R., Huang, Y.Z., Xie, Y., Liu, G., Saito, H., Tang, H., Wen, L., Brady-Kalnay, S.M., Mei, L., Wu, J.Y., Xiong, W.C. and Rao, Y. (2001) Signal Transduction in Neuronal Migration: Roles of GTPase Activating Proteins and the Small GTPase Cdc42 in the Slit-Robo Pathway. Cell, 107, 209-221.
http://dx.doi.org/10.1016/S0092-8674(01)00530-X
[15] Klein, R. (2004) Eph/Ephrin Signaling in Morphogenesis, Neural Development and Plasticity. Current Opinion in Cell Biololgy, 16, 580-589. http://dx.doi.org/10.1016/j.ceb.2004.07.002
[16] Govek, E.E., Newey, S.E. and Van Aelst, L. (2005) The Role of the Rho GTPases in Neuronal Development. Genes & Development, 19, 1-49. http://dx.doi.org/10.1101/gad.1256405
[17] Murai, K.K. and Pasquale, E.B. (2005) New Exchanges in Eph-Dependent Growth Cone Dynamics. Neuron, 46, 161-163. http://dx.doi.org/10.1016/j.neuron.2005.04.004
[18] Shi, L., Fu, A.K. and Ip, N.Y. (2010) Multiple Roles of the Rho GEF Ephexin1 in Synapse Remodeling. Communicative & Integrative Biology, 3, 622-624. http://dx.doi.org/10.4161/cib.3.6.13481
[19] Chang, I.Y., Ohn, T., Ko, G.S., Yoon, Y., Kim, J.W. and Yoon, S.P. (2012) Immunolocalization of Steroidogenic Acute Regulatory Protein-Related Lipid Transfer (START) Domain-Containing Proteins in the Developing Cerebellum of Normal and Hypothyroid Rats. Journal of Chemical Neuroanatomy, 43, 28-33.
http://dx.doi.org/10.1016/j.jchemneu.2011.10.003
[20] Tolbert, D.L. and Clark, B.R. (2000) Olivocerebellar Projections Modify Hereditary Purkinje Cell Degeneration. Neuroscience, 101, 417-433. http://dx.doi.org/10.1016/S0306-4522(00)00362-6
[21] Simat, M., Ambrosetti, L., Lardi-Studler, B. and Fritschy, J.M. (2007) GABAergic Synaptogenesis Marks the Onset of Differentiation of Basket and Stellate Cells in Mouse Cerebellum. The European Journal of Neuroscience, 26, 2239-2256. http://dx.doi.org/10.1111/j.1460-9568.2007.05846.x
[22] Haag, N., Schwintzer, L., Ahuja, R., Koch, N., Grimm, J., Heuer, H., Qualmann, B. and Kessels, M.M. (2012) The Actin Nucleator Cobl Is Crucial for Purkinje Cell Development and Works in Close Conjunction with the F-Actin Binding Protein Abp1. The Journal of Neuroscience, 32, 17842-17856.
http://dx.doi.org/10.1523/JNEUROSCI.0843-12.2012
[23] Oostland, M. and van Hooft, J.A. (2013) The Role of Serotonin in Cerebellar Development. Neuroscience, 248C, 201-212. http://dx.doi.org/10.1016/j.neuroscience.2013.05.029
[24] Schmucker, D. and Zipursky, S.L. (2001) Signaling Downstream of Eph Receptors and Ephrin Ligands. Cell, 105, 701-704. http://dx.doi.org/10.1016/S0092-8674(01)00391-9
[25] Rosas, O.R., Figueroa, J.D., Torrado, A.I., Rivera, M., Santiago, J.M., Konig-Toro, F. and Miranda, J.D. (2011) Expression and Activation of Ephexin Is Altered after Spinal Cord Injury. Developmental Neurobiology, 71, 595-607.
http://dx.doi.org/10.1002/dneu.20848

  
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