Feeding Study with Bt Corn (MON810: Ajeeb YG) on Rats: Biochemical Analysis and Liver Histopathology


Bt corn MON810: Ajeeb YG produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart Ajeeb. Further, feeding studies on rats were designed to complete assessing the safety of MON810: Ajeeb YG. Three groups of rats (6 males and 6 females rats/group) were fed on control diet, non-Bt corn and Bt corn for 1.5 months and 3 months. After 1.5 months, 3 males and 3 females from each group were sacrificed, and after another 1.5 months, 3 males and 3 females from each group and their offspring were sacrificed. Histopathological examination, blood haematology and serum biochemical analysis were determined. Results indicated significant differences among the tested parameters in the three groups especially Bt group. Severe changes in the liver of Bt group after 3 months were observed.

Share and Cite:

E. Abdo, O. Barbary and O. Shaltout, "Feeding Study with Bt Corn (MON810: Ajeeb YG) on Rats: Biochemical Analysis and Liver Histopathology," Food and Nutrition Sciences, Vol. 5 No. 2, 2014, pp. 185-195. doi: 10.4236/fns.2014.52024.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] C. James, “Global Status of Commercialized Biotech/GM Crops: 2012,” ISAAA, 2012, Brief No. 44, p. 18.
[2] A. M. Rayan, A. A. Gab-Alla, Z. S. El-Shamei, A. A. Shatta and E. A. Moussa, “Morphological and Biochemical Changes in Male Rats Fed on Genetically Modified Corn (Ajeeb YG),” Journal of American Science, Vol. 8, No. 9, 2012, pp. 1117-1123.
[3] K. R. Ostlie, et al., “Bt Corn & European Corn Borer: Long-Term Success through Resistance Management,” 2008.
[4] A. Tenuta, M. Sears, F. Meloche and A. Schaafsma, “A Grower’s Handbook Controlling European Corn Borer with BT Corn Technology,” 2011, pp. 1-16.
[5] OECD, “Consensus Document on Safety Information on Transgenic Plants Expressing Bacillus Thuringiensis— Derived Insect Control Proteins,” Organization for Economic Cooperation and Development, Vol. 42, 2007, pp. 1-110.
[6] EFSA, “Guidance Document of the Scientific Panel on Genetically Modified Organisms for the Risk Assessment of Genetically Modified Plants and Derived Food and Feed,” European Food Safety Authority Journal, Vol. 99, 2006, pp. 1-100.
[7] EFSA, “Review on Safety & Nutritional Assessment of Genetically Modified Plant and Derived Food and Feed: The Role of Animal Feeding Trials, Report of the EFSA GMO Panel Working Group on Animal Feeding Trials,” Food and Chemical Toxicology, Vol. 46, Suppl. 1, 2008, pp. S2-S70. http://dx.doi.org/10.1016/j.fct.2008.02.008
[8] EFSA, “Scientific Opinion of the Panel on Genetically Modified Organisms (GMO) on Guidance for Risk Assessment of Food and Feed from Genetically Modified Plants,” European Food Safety Authority Journal, Vol. 9, No. 5, 2011, pp. 1-37.
[9] EC, “Regulation (EC) 1829/2003 of the European Parliament and of the Council of 22 September 2003 on Genetically Modified Food and Feed,” Official Journal of the European Communities, Vol. L268, 2003, pp. 1-23.
[10] E. M. Abdo, O. M. Barbary and O. E. Shaltout, “Chemical Analysis of BT Corn ‘Mon-810: Ajeeb-YG®’ and Its Counterpart Non-Bt Corn ‘Ajeeb’,” Journal of Applied Chemistry, Vol. 4, No. 1, 2013, pp. 55-60.
[11] FOSIE, “Food Safety in Europe: Risk Assessment of Chemicals in Food and Diet,” Food Chemical & Toxicology, Vol. 40, No. 2, 2002, pp. 139-427.
[12] G. E. Séralini, R. Mesnage, E. Clair, S. Gress, J. S. de Vendomois and C. D. Dominique, “Genetically Modified Crops Safety Assessments: Present Limits and Possible Improvements,” Environmental Sciences of European, Vol. 23, No. 1, 2011, pp. 1-10.
[13] A. Kilic and T. M. Akay, “A Three Generation Study with Genetically Modified Bt Corn in Rats: Biochemical and Histopathological Investigation,” Food and Chemical Toxicology, Vol. 46, No. 3, 2008, pp. 1164-1170.
[14] S. I. Taluker, “Histopathology Techniques: Tissue Processing and Staining,” Mymensingh Medical College, Department of Pathology, Mymensingh, 2007, p. 11.
[15] B. Hammond, R. Dudekb, J. Lemena and M. Nemetha, “Results of a 13 Week Safety Assurance Study with Rats Fed Grain from Glyphosate Tolerant Corn,” Food and Chemical Toxicology, Vol. 42, No. 6, 2004, pp. 10031014. http://dx.doi.org/10.1016/j.fct.2004.02.013
[16] AACC, “Liver Profile,” 2013.
[17] S. Adamu, et al., “Changes in the Serum Profiles of Lipids and Cholesterol in Sheep Experimental Model of Acute African Trypanosomosis,” African Journal of Biotechnology, Vol. 7, No. 2, 2008, pp. 2090-2098.
[18] D. K. Rai, P. K. Rai, A. Gupta, G. Watal and B. Sharma, “Cartap and Carbofuran Induced Altrations in Serum Lipid Profile of Wistar Rats,” Indian Journal of Clinical Biochemistry, Vol. 24, No. 2, 2009, pp. 198-201.
[19] R. Kroes, J. M. Sontag, S. Sell, G. M. Williams and J. H. Weisburger, “Elevated Concentrations of Serum AlphaFetoprotein in Rats with Chemically Induced Liver Tumors,” Cancer Research, Vol. 35, No. 5, 1975, pp. 12141217.
[20] J. L. Domingo, “Toxicity Studies of Genetically Modified Plants: A Review of the Published Literature,” Critical Reviews in Food Science and Nutrition, Vol. 47, No. 8, 2007, pp. 721-733.
[21] R. Teshima, H. Akiyama, H. Okunuki, J. Sakushima, Y. Goda, H. Onodera, J. Sawada and M. Toyoda, “Effect of GM and Non-GM Soybeans on the Immune System of BN Rats and B10A Mice,” Journal of the Food Hygienic Society of Japan, Vol. 41, No. 3, 2000, pp. 188-193.
[22] AACC, “Complete Blood Count,” 2013.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.