Salivary Amino Acids Determination and Their Changes in Vision Stress Experiments

Wei Tang, Xuesong Li, Xiaoxiao Wu, Yu Wang, Xuejun Kang

Key Laboratory of Child Development and Learning Science of Ministry of Education, Research Center for Learning Science, Southeast University, Nanjing, China 2Suzhou Key Laboratory of Enviroment and Biosafety, Southeast University, Suzhou, China.

DOI: 10.4236/jss.2013.16005                

Abstract

Amino acid neurotransmitters represent a major class of compounds that are involved in neuronal communication at CNS synapses, which can provide the basis for a variety of disease diagnosis and treatments and the study of the mechanism of mental illness. An analytical method for the determination of several amino acids in saliva was established with reversed-phase high performance liquid chromatography (RP- HPLC) with UV detector. About ten kinds of amino acids were detected in saliva. Nine subjects have participated in the stress experiments which have undergone a 50-min three-dimensional cartoon watching. The result of the experiment has proved that four kinds of salivary amino acids respond to the vision stress experiment obviously

Keywords

Amino Acids; RP-HPLC; Stress Experiments

Conflicts of Interest

The authors declare no conflicts of interest.

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