The Effects of Auxins and Cytokinin on Growth and Development of (Musa sp.) Var. “Yangambi” Explants in Tissue Culture

Munguatosha Ngomuo, Emerald Mneney, Patrick Ndakidemi

Mikocheni Agricultural Research Institute, Dar es Salaam, Tanzania.
School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.

DOI: 10.4236/ajps.2013.411269                

Abstract

The aim of this study was to investigate the effects of concentration of different growth regulators (auxins and cytokinins) on growth and development of banana shoot tips cultured in vitro. Explants were taken from young suckers of field grown plants of var. “Yangambi”. The shoot tips were cultured on MS media supplemented with different concentrations of BAP (0, 2, 4, 6 and 8 mg/l) with or without IAA at concentration of 0.34 mg/l. At the rooting phase, the media was supplemented with different concentrations of IBA (0.1, 0.5, 1.0, 1.5 and 2.0 mg/l) with or without BAP at concentration of 0.2 mg/l. The results indicated that 6.0 mg/l BAP significantly increased the number of shoots formed and the interaction of 6 mg/l BAP with 0.35 mg/l IAA significantly increased the fresh weight. For rooting, 2.0 mg/l IBA was more efficient in number and length of roots produced than all other treatments.

Keywords

Bud’s Proliferation; Fresh Weight; In-Vitro Rooting; Root Length; Micro Propagation; Shoot Length; Var. “Yangambi”

Conflicts of Interest

The authors declare no conflicts of interest.

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