Production of low cost Bacillus thuringiensis based-biopesticide for management of chickpea pod-borer Helicoverpa armigera (Huebn) in Pakistan


The biopesticide was prepared from locally available low cost ingredients: dried beef blood, molasses and mineral salts (ZnCl2, MgCl2, MnCl2, CaCl2CaCl2, and FeCl3) which were used as medium for the laboratory scale production of Bacillus thuringiensis (B.t.) bio-pesticide by shake flask technique. Indigenous B.t. isolate PA-Sb- 46.3 which produced two crystals—bi-pyramidal and cuboidal was found 73.6 times toxic against H. armigera than reference strain Bacillus thuringiensis var. kurstaki (HD-I-S-1980) used. Medium was fermented for 72 hours at 30℃ ± 2℃ and 160 rpm. 72 h fermented medium showed 95% - 99% sprulation, with spore yield of 3.97 × 109 spores/ml, and LC50 value to 1st instar larvae of H. armigera was 0.53 μg/ml diet. Preservatives and diluents used in the biopesticide were found to be effective to store at room temperature over a period of 30 months. These observations suggested that the biopesticide produced was effective and highly economical for the industrial scale production to manage H. armigera in Pakistan.

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Bibi, A. , Ahmed, K. , Ayub, N. and Alam, S. (2013) Production of low cost Bacillus thuringiensis based-biopesticide for management of chickpea pod-borer Helicoverpa armigera (Huebn) in Pakistan. Natural Science, 5, 1139-1144. doi: 10.4236/ns.2013.511140.

Conflicts of Interest

The authors declare no conflicts of interest.


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