Phytochemical Profiling with Antioxidant and Antimicrobial Screening of Amaranthus viridis L. Leaf and Seed Extracts

Saud Asif Ahmed, Sumaira Hanif, Tehreema Iftkhar

Department of Botany, GC University, Faisalabad, Pakistan.
Enzyme Biotechnology Lab., Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan.

DOI: 10.4236/ojmm.2013.33025                

Abstract

Methanol extracts of the dried leaves and seeds of Amaranthus viridis were collected and used for phytochemicals and antibacterial analysis. By detecting the MIC and zone inhibition, the antibacterial activity was determined against different bacterial and fungal strains. The extract yields from the leaves and seeds ranged 5.5-6.1 and 2.42%-3.72% w/w, respectively. Phytochemical investigation of this plant determines that tanins (6.07%-5.96%), saponins (53%-32%), alkaloids (13.14% - 11.42%), protiens (16.76%-24.51%) and glycosides (63.2%-32.3%) were rich in leaves. The extracts also contained appreciable levels of total phenolic contents (2.81-3.61 GAE, g/100 g), total flavanoid contents (18.4-5.42 QE, g/100 g) and DPPH free radical scavenging activity, showing IC₅₀ (83.45-75.95 μg/mL) along with reducing power was calculated. The MIC of extracts ranged 178 - 645 μg/mL. The results of this study suggest the possibility of using the methanolic extracts in treating the diseases caused by the test organisms.

Keywords

Amaranthus viridis; Phytochemicals; DPPH. Lineolic Acid; Reducing Power; Leaf and Seed Extract

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	A, Ghani, E. M. Abdulrahman and J. A. Onaolapo, “Chemical and Microbiological Evaluation of Some Nigerian Traditional Preparations,” The Report, Nigeria, 1989.
[2]	J. Robbers, M. Speedie and V. Tyler, “Pharmacognosy and Pharmacobiotechnology,” Williams, Wilkins Baltimore, 1996.
[3]	M. M. Iwu, A. R. Duncan and C. O. Okunji, “New Antimicrobials of Plant Origin,” In: J. Janick, Ed., Prospective on New Crops and New Uses, ASHS Press, Alexandria, 1999.
[4]	M. C. Castello, P. Anita, C. Naresh and S. Madhuri, “Antimicrobial Activity of Crude Extracts from Plant Parts and Corresponding Calli of Bixaorellina. Ind,” Journal of Experimental Biotechnology, Vol. 40, No. 12, 2002, pp. 1378-1381.
[5]	A. Ghani, “Introduction to Pharmacognosy,” Ahmadu Bello University Press, Nigeria, 1990.
[6]	I. N. Dobelis, “Magic and Medicine of Plants,” The Readers Digest Association Inc., New York, 1993.
[7]	G. F. Stallknecht and J. R. Schulz-Schaeffer, “Amaranth Rediscovered New Crops,” Wiley, New York, 1993.
[8]	O. Ladeji, Z. S. Okoye and T. Ojobe, “Chemical Evaluation of the Nutritive Value of Leaf of Fluted Pumpkin,” Food Chemistry, Vol. 53, No. 4, 1995, pp. 353-355. doi:10.1016/0308-8146(95)99826-L
[9]	O. Polterait, “Antioxidants and Free-Radical Scavengers of Natural Origin,” Current Organic Chemistry, Vol. 1, No. 4, 1997, pp. 415-440.
[10]	J. K. Willcox, S. L. Ash and G. L. Catignani, “Antioxidants and Prevention of Chronic Diseases,” Critical Reviews in Food Science and Nutrition, Vol. 44, No. 4, 2004, pp. 275-295. doi:10.1080/10408690490468489
[11]	H. M. Burkill, “The Useful Plants of West Tropical Africa,” Royal Botanical Garden Kew, London, 1984.
[12]	T. O. Odugbemi, “Outlines and Pictures of Medicinal Plants from Nigeria,” University of Lagos Press, Nigeria, 2006.
[13]	R. Y. Nsimba, H. Kikuzaki and Y. Konishi, “Antioxidant Activity of Various Extracts and Fractions of Chonopodium quinoa and Amaranthus Species Seed,” Food Chemistry, Vol. 106, No. 2, 2008, pp. 760-766. doi:10.1016/j.foodchem.2007.06.004
[14]	M. G. Abukakar, A. N. Ukwuani and R. A. Shehu, “Phytochemical Screening and Antibacterial Activity of Tamarindus indica Pulp Extract,” Asian Journal of Biochemistry, Vol. 3, No. 2, 2008, pp. 134-138. doi:10.3923/ajb.2008.134.138
[15]	H. S. M. Fallah, H. R. Alavian, M. R. Heydari and K. Abolmaali, “The Efficacy of Liv-52 on Liver Cirrhotic Patients: A Randomized, Double Blind, Placebo-Controlled First Approach,” Journal of Phytomedicine, Vol. 12, No. 9, 2005, pp. 619-624. doi:10.1016/j.phymed.2004.10.003
[16]	K. H. Kyung, K. M. Jeong, C. H. Yon, K. Eun-Ki and S. D. Hoon, “Antioxidant and Antidiabatic Effects of Amaranth (Amaranthus cruentus) in Diabatic Rat,” Cell Biochemistry and Function, Vol. 24, No. 3, 2006, p. 1.
[17]	S. Yue and H. Sun, “The Characteristics and Prospects of Amaranthus Food in China,” The 2nd International Symposium on New and Nononventional Plants: Their Perspective Use, Putstchino, 1997, pp. 138-139.
[18]	A. Sofowora, “Medicinal Plant and Traditional Medicine in Africa,” Wiley and Sons Limited, Chichester, 1993.
[19]	J. B. Harborne, “A Guide to Modern Techniques of Plant Analysis,” Chapman and Hall, London, 1973.
[20]	V. Tyler, “Phytomedicines in Western Europe: Their Potential Impact on Herbal Medicine in the United States,” Herbalgram, Vol. 30, 1994, pp. 24-30.
[21]	W. C. Evans, “Trease and Evans Phamacognosy,” Bailere Traiadal, London, 1989.
[22]	B. O. Obadoni and P. O. Ochuka, “Phytochemical Studies and Comparative Efficacy of the Crude Extracts of Some Homeostatic Plants in Edo and Delta States of Nigeria,” Global Journal of Pure Applied Sciences, Vol. 8, No. 2, 2001, pp. 203-208.
[23]	V. Dewanto, K. K. Adom and R. H. Liu, “Thermal Processing Enhances the Nutritional Value of Tomatoes By Increasing Total Antioxidant Activity,” Journal of Agricultural and Food Chemistry, Vol. 50, No. 10, 2002, pp. 3010-3014. doi:10.1021/jf0115589
[24]	P. R. Miliauskas, Venskutonis and T. A. Van Beek, “Screening of Radical Scavenging Activity of Some Medicinal and Aromatic Plant Extracts,” Journal of Food Chemistry, Vol. 85, No. 2, 2004, pp. 231-237. doi:10.1016/j.foodchem.2003.05.007
[25]	J. C. Lee, H. R. Kim and K. J. Jim, “Antioxidant Property of an Extract of the Stem of Opuntia Ficus-Indica Var. saboten,” Journal of Agricultural and Food Chemistry, Vol. 40, No. 4, 2002, pp. 6490-6496. doi:10.1021/jf020388c
[26]	A. Yildrim, A. Mavi and A. A. Kara, “Determination of Antioxidant and Antimicrobial Activities of Rumex Crispus L. Extracts,” Journal of Agricultural and Food Chemistry, Vol. 49, No. 8, 2001 pp. 4083-4089. doi:10.1021/jf0103572
[27]	National Committee for Clinical Laboratory Standards, “Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically,” National Committee for Clinical Laboratory Standards, Wayne, 2000.
[28]	N. Ozsoy, O. K. Tilmaz and C. R. Yanardag, “In Vitro Antioxidant Activity of Amaranthus lividus L.,” Journal of Food Chemistry, Vol. 116, No. 4, 2009, pp. 867-872. doi:10.1016/j.foodchem.2009.03.036
[29]	O. A. Odukoya, S. I. Inya-Agha, F. I. Segun, M. O. Sofidiya and O. O. Ilori, “Antioxidant Activity of Selected Nigerian Green Leafy Vegetable,” American Journal of Food Technology, Vol. 2, No. 3, 2007, pp. 169-175. doi:10.3923/ajft.2007.169.175
[30]	A. Lipkin, A. Veronika, N. Aleksandra, B. Aleksey, K. Eberhardt, L. B. Mikhae, G. Eugene and E. Tsezi, “An Antimicrobial Peptide Ar-AMP from Amaranth (Amaranthus retroflexus L.) Seeds,” Journal of Science Direct, Vol. 32, No. 1, 2004, pp. 93-95.
[31]	Y. Z. Cai, M. Sun and H. Croke, “Characterization and Application of Betalian Pigment of Plant Amaranthaceae,” Trend in food and Science Technology, Vol. 16, No. 9, 2005, pp. 370-376.