Generation of Unfolded DNA in Human Neutrophils Following Hypothermal Treatment

Jin Kawata, Makoto Kikuchi, Hisato Saitoh

Department of New Frontier Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
Kumamoto Health Science University, Kumamoto, Japan.

DOI: 10.4236/cellbio.2013.23013                

Abstract

By visualizing DNA with diamidino phenylindole (DAPI), we found that hypothermal incubation followed by rewarming of human neutrophils resulted in an increased number of DAPI-positive objects representative of extensive DNA unfolding seemingly similar to neutrophil extracellular traps (NETs). In contrast to canonical NET formation, diphenylene iodonium (DPI), an NADPH oxidase inhibitor, exhibited negligible effects on formation of the DAPI-positive objects. Moreover, multiple instances of DNA damage were detected in the objects, but not in canonical NETs. Our results thus suggest the potential of hypothermia for triggering DNA structural alteration in neutrophils, which is similar to but distinct from NET formation.

Keywords

Hypothermal Treatment; DNA Unfolding; Neutrophil Extracellular Trap (NET)

Conflicts of Interest

The authors declare no conflicts of interest.

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