Selection of Highly Susceptible Cell Lines to Foot and Mouth Disease Virus Infection

O. Zabal, N. Fondevila

.
Instituto de Virologia, CICVyA, INTA, Dr. Nicolas Repetto y De los Reseros s/n, Hurlingham, Argentina.

DOI: 10.4236/ojvm.2013.35042                

Abstract

Different non-established cultures were examined to find those that showed high sensitivity to Foot and Mouth Disease Virus (FMDV). Ovine kidney cultures showed high sensitivity to types A, O and C, and were suitable to detect viral infection in samples of animal, as well as cell culture origin. The level of detection in this system was up to ten times higher than in BHK₂₁ cells, which were commonly used for FMDV isolation and production. Viral production levels in ovine kidney cultures ranged from similar to twice as high as in BHK₂₁. Ovine kidney cultures maintained these characteristics for at least 18 passages, allowing their use as an alternative system for FMDV diagnoses.

Keywords

Foot-and-Mouth Disease; Tissue Culture; Virus Isolation

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	OIE, “Foot and Mouth Disease,” In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2.1.5, OIE, 2012.
[2]	J. B. Clarke and R. E. Spier, “Variation in the Susceptibility of BHK Populations and Cloned Cell Lines to Three Strains of Foot-and-Mouth Disease Virus,” Archives of Virology, Vol. 63, No. 1, 1980, pp. 1-9. doi:10.1007/BF01320756
[3]	M. P. De Castro, “An Infectious Agent Causing ‘Spontaneous’ Degeneration of Swine Cells in Vitro,” In Vitro, Vol. 9, No. 1, 1973, pp. 8-16. doi:10.1007/BF02615982
[4]	N. P. Ferris, G. H. Hutchings, H. J. Moulsdale, J. Golding and J. B. Clarke, “Sensitivity of Primary Cells Immortalised by Oncogene Transfection for the Detection and Isolation of Foot-and-Mouth Disease and Swine Vesicular Disease Viruses,” Veterinary Microbiology, Vol. 84, No. 4, 2002, pp. 307-316. doi:10.1016/S0378-1135(01)00469-2
[5]	N. P. Ferris, D. P. King, S. M. Reid, G. H. Hutchings, A. E. Shaw, D. J. Paton, N. Goris, B. Haas, B. Hoffmann, E. Brocchi, et al., “Foot-and-Mouth Disease Virus: A First Inter-Laboratory Comparison Trial to Evaluate Virus Isolation and RT-PCR Detection Methods,” Veterinary Microbiology, Vol. 117, No. 2-4, 2006, pp. 130-140. doi:10.1016/j.vetmic.2006.06.001
[6]	L. J. M. Reed, “A Simple Method of Estimating Fifty Percent Endpoints,” The American Journal of Hygiene, Vol. 27, 1938, pp. 493-497.
[7]	T. Jackson, A. P. Mould, D. Sheppard and A. M. King, “Integrin Alphavbeta1 Is a Receptor for Foot-and-Mouth Disease Virus,” Journal of Virology, Vol. 76, No. 3, 2002, pp. 935-941. doi:10.1128/JVI.76.3.935-941.2002
[8]	T. Jackson, A. M. King, D. I. Stuart and E. Fry, “Structure and Receptor Binding,” Virus Research, Vol. 91, No. 1, 2003, pp. 33-46. doi:10.1016/S0168-1702(02)00258-7