Protective effect of maifanite against cadmium-induced oxidative stress to rats hippocampus by regulating the balance and metabolism of metals

Hailin Liao, Lingfeng Jiang, Hongmiao Huang, Lixia Zhou, Ling Li, Shixian Cheng

Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.

DOI: 10.4236/health.2013.59187                

Abstract

In this paper, the author reported the effect of maifanite on the learning and memory of rats suffering Cd poisoning by step-down and step-through test, analyzed the content of MDA, MT, SOD, and GSH-Px in the hippocampus of rats respectively, and assayed the content of Fe, Cu, Zn, Mn, Se, and Cd in the hippocampus of rats. In the step-down test, it is found that maifanite can reduce the number of electric shock, prolong step-down latency, and cut down error times when rats are exposed to Cd 24 h. In the step-through test, maifanite can obviously prolong the latency of entering the dark cabin of rats suffering Cd poisoning. The exogenous Cd can lead to the decrease of SOD and GSH-Px activity and the increase of MDA and MT content. The alternation of maifanite and Cd by the gavage can significantly increase the SOD and GSH-Px activity as well as MT content, while lessening the MDA content. The accumulation of exogenous Cd in hippocampus causes the increase of Zn, Fe, Mn, and Cd content, and the decrease of Cu and Se content. The intake of maifanite can regulate the imbalance of metals content and make its content near to the of level control group. The research shows that maifanite can improve the ability of learning and memory of rats suffering Cd poisoning, increase MT formation and lower hyperoxidative damage. Maifanite has the function of balancing the content of trace elements in rats suffering Cd poisoning, and meanwhile can alleviate the toxicity of Cd effectively.

Keywords

Maifanite; Cd Toxicity; Oxidative Stress; Metallothionein; Trace Element

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Hideaki, S., Yasutake, A., Hirashima, T., Takamure, Y., Kitano, T., Waalkes, M.P. and Imamura, Y. (2008) Strain difference of cadmium accumulation by liver slices of inbred Wistar-Imamichi and Fischer 344 rats. Toxicology in Vitro, 22, 338-343. doi:10.1016/j.tiv.2007.09.013
[2]	Lazarus, M. (2010) Cadmium and selenium interaction in mammals. Arhiv za Higijenu Rada i Toksikologiju, 61, 357-369. doi:10.2478/10004-1254-61-2010-2021
[3]	Michalke, B., Halbach, S. and Nischwitz, V. (2009) JEM spotlight: Metal speciation related to neurotoxicity in humans. Journal of Environmental Monitoring, 11, 939-954. doi:10.1039/b817817h
[4]	Juan, L., Zhang, P.Y., Gao, Y., Song, X.G. and Dong, J.H. (2008) Overview of maifanshi: Its Physi-chemical properties and nutritious function in drinking water. Environmental Science & Technology, 31, 63-66.
[5]	Zhang, B.G. (2005) The Progress of pharmaceutical research on maifanite. Chinese Traditional Patent Medicine, 27, 1205-1208.
[6]	Liu, Z.Y., Liu, Q., Ma, J.K. and Zhang, C.W. (1986) Study on pharmacologic action of China maifanite. Jilin Journal of Traditional Chinese Medicine, 4, 28-30.
[7]	Jiang, L.F., Liao, H.L., Huang, H.M., Zhou, L.X., Li, L., Cheng, S.X. and Du, C.Z. (2013) Potential prevention and treatment of maifanite for Alzheimer’s Disease based on behavior test, oxidative stress assay and trace element analysis in hippocampus of Abeta (25-35)-induced AD rats. Biological Trace Element Research, 152, 50-56. doi:10.1007/s12011-012-9590-7
[8]	Kokaia, Z. and Lindvall, O. (2003) Neurogenesis after ischaemic brain insults. Current Opinion in Neurobiology, 13, 127-132. doi:10.1016/S0959-4388(03)00017-5
[9]	Xu, S.Y., Bian, R.L. and Chen, X. (2002) Experimental protocols in pharmacology. 3rd Edition, People’s Medical Publishing House, Beijing, 826-830.
[10]	Leonard, S.S., Harris, G.K. and Shi, X.L. (2004) Metal-induced oxidative stress and signal transduction. Free Radical Biology and Medicine, 37, 1921-1942. doi:10.1016/j.freeradbiomed.2004.09.010
[11]	Cousins, R.J. (1985) Absorption, transport, and hepatic metabolism of copper and zinc: Special reference to metallothionein and ceruloplasmin. Physiological Reviews, 65, 238-309.
[12]	Waalkes, M.P. and Goering, P.L. (1990) Metallothionein and other cadmium-binding proteins: Recent developments. Chemical Research in Toxicology, 3, 281-288. doi:10.1021/tx00016a001
[13]	Klaassen, C.D. and Liu, J. (1998) Induction of metallothionein as an adaptive mechanism affecting the magnitude and progression of toxicological injury. Environmental Health Perspectives, 106, 297-300.
[14]	Gemma, C., Vila, J., Bachstetter, A. and Bickford, P.C. (2007) Oxidative stress and the aging brain: From theory to prevention. In: Riddle, D.R., Ed., Brain Aging: Models, Methods, and Mechanisms. CRC Press, Boca Raton.
[15]	Batcioglu, K., Ozturk, C., Karagozler, A. and Karatas, F. (2002) Comparison of the selenium level with GSH-Px activity in the liver of rats treated with 7,12 DMBA. Cell Biochemistry and Function, 20, 115-118. doi:10.1002/cbf.958
[16]	Webb, M. and Cain, K. (1982) Commentary: Functions of metallothionein. Biochemical Pharmacology, 31, 137-142. doi:10.1016/0006-2952(82)90202-7
[17]	Klaassen, C.D., Liu, J. and Diwan, B.A. (2009) Metallothionein protection of cadmium toxicity. Toxicology and Applied Pharmacology, 238, 215-220. doi:10.1016/j.taap.2009.03.026