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In vitro differentiation of human umbilical cord-derived mesenchymal stem cells into CD34+ cells via CD34 antibody

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DOI: 10.4236/jbise.2013.68A1005    3,546 Downloads   5,263 Views  

ABSTRACT

CD34+cells differentiated from mesenchymal stem cells (MSCs) have a strong biological function in cardiovascular regeneration. However, the molecular mechanisms of and the methods to improve the CD34+ cell differentiation from MSCs, especially from human MSCs (hUC-MSCs) are still unclear. In the current study, the effect of CD34 antibody on the CD34+ cell differentiation from human umbilical cord (UC)-derived MSCs (hUC-MSCs) is determined. The results have demonstrated that the expression of cd34 protein is significantly increased in hUC-MSCs treated with CD34 antibody. In addition, the cell proliferation is increased in hUC-MSCs after treatment with CD34 antibody. Moreover, the expression of PI3K, AKT, p-AKT proteins, which are signaling molecules related to stem cell differentiation, is increased by CD34 antibody. The results suggest that CD34 antibody could promote the differentiation of hUC-MSCs into CD34+ cells and PI3K/AKT may be involved in this important process.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Guo, S. , Guo, L. , Sun, M. , Ma, W. , Lu, Y. and Liu, Y. (2013) In vitro differentiation of human umbilical cord-derived mesenchymal stem cells into CD34+ cells via CD34 antibody. Journal of Biomedical Science and Engineering, 6, 53-58. doi: 10.4236/jbise.2013.68A1005.

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