Structural Investigation of Cell Wall Mannan Antigen Obtained from Pathogenic Yeast Candida zeylanoides


Antigenicity of pathogenic yeast Candida zeylanoides NBRC 10326 was found to have similar to that of Candida guilliermondii, but it was slightly different, i.e. NBRC 10326 strain cells react strongly with the factor antibodies 1, 4, and 9, and react relatively weakly with factor antibody 5. We have investigated the structural characteristic of cell wall mannan of this strain using a non-degradable method, nuclear magnetic resonance analysis. The results obtained from NMR analysis, the two-dimensional homonuclear Hartmann-Hahn, indicated that C. zeylanoides mannan contains α-1, 3-linked and β-1,2-linked oligomannosyl side chains corresponding to C. guilliermondii specific antigenic factor 9. In addition, it was found that the mannan contains β-1,2-linked oligomannosyl side chains attached to phosphate groups corresponding to anitigenic factor 5, which were not found in C. guilliermondii mannan.

Share and Cite:

H. Kobayashi, S. Kawakami, Y. Ogawa, N. Shibata and S. Suzuki, "Structural Investigation of Cell Wall Mannan Antigen Obtained from Pathogenic Yeast Candida zeylanoides," Open Journal of Medical Microbiology, Vol. 3 No. 2, 2013, pp. 139-143. doi: 10.4236/ojmm.2013.32021.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Y. Fukazawa, T. Shinoda and T. Tsuchiya, “Response and Specificity of Antibodies for Candida albicans,” Journal of Bacteriology, Vol. 95, No. 3, 1968, pp. 754-763.
[2] T. Tsuchiya, Y. Fukazawa and S. Kawakita, “A Method for the Rapid Identification of the Genus Candida,” Mycopathologia, Vol. 10, No. 3, 1959, pp. 191-206.
[3] T. Tsuchiya, Y. Fukazawa, M. Taguchi, T. Nakase and T. Shinoda, “Serologic Aspects on Yeast Classification,” Mycopathologia, Vol. 53, No. 1-4, 1974, pp. 77-92.
[4] T. Shinoda, L. Kaufman and A. A. Padhye, “Comparative Evaluation of the Iatron Serological Candida Check Kit and the API 20C Kit for Identification of Medically Important Candida Species,” Journal of Clinical Microbiology, Vol. 13, No. 3, 1981, pp. 513-518.
[5] N. Shibata, H. Kobayashi and S. Suzuki, “Immunochemistry of Pathogenic Yeast Candida Species, Focusing on Mannan,” Proceeding of the Japanese Academy Series B: Physical and Biological Sciences, Vol. 88, No. 6, 2012, pp. 250-265. doi:10.2183/pjab.88.250
[6] H. Kobayashi, H. Oyamada, K. Matsuda, N. Shibata and S. Suzuki, “Distribution of Antigenic Oligomannosyl Side Chains in the Cell Wall Mannans of Several Strains of Candida tropicalis,” Archives of Microbiology, Vol. 180, No. 1, 2003, pp. 76-80. doi:10.1007/s00203-003-0550-7
[7] N. Shibata, R. Akagi, T. Hosoya, K. Kawahara, A. Suzuki, K. Ikuta, H. Kobayashi, K. Hisamichi, Y. Okawa and S. Suzuki, “Existence of Novel Branched Side Chains Containing β-1,2 and α-1,6 Linkages Corresponding to Antigenic Factor 9 in the Mannan of Candida guilliermondii,” Journal of Biological Chemistry, Vol. 271, No. 16, 1996, pp. 9259-9266. doi:10.1074/jbc.271.16.9259
[8] N. Shibata, M. Arai, E. Haga, T. Kikuchi, M. Najima, T. Satoh, H. Kobayashi and S. Suzuki, “Structural Identification of an Epitope of Antigenic Factor 5 in Mannans of Candida albicans NIH B-792 (Serotype B) and C. albicans J-1012 (Serotype A) as β-1,2-Linked Oligomannosyl Residues,” Infection and Immunity, Vol. 60, No. 10, 1992, pp. 4100-4110.
[9] H. Kobayashi, N. Shibata and S. Suzuki, “Evidence for Oligomannosyl Residues Containing both β-1,2 and α-1,2 Linkages as a Serotype A-Specific Epitope(s) in Mannans of Candida albicans,” Infection and Immunity, Vol. 60, No. 5, 1992, pp. 2106-2109.
[10] M. Funayama, A. Nishikawa, T. Shinoda and Y. Fukazawa, “Immunochemical Determinant of Candida parapsilosis,” Carbohydrate Reearchs, Vol. 117, 1983, pp. 229- 239. doi:10.1016/0008-6215(83)88089-6
[11] N. Shibata, K. Ikuta, T. Imai, Y. Satoh, R. Satoh, A. Suzuki, C. Kojima, H. Kobayashi, K. Hisamichi and S. Suzuki, “Existence of Branched Side Chains in the Cell Wall Mannan of Pathogenic Yeast, Candida albicans. Structure-Antigenicity Relationship between the Cell Wall Mannans of Candida albicans and Candida parapsilosis,” Journal of Biological Chemistry, Vol. 270, No. 3, 1995, pp. 1113-1122. doi:10.1074/jbc.270.3.1113
[12] H. Kobayashi, H. Oyamada, A. Suzuki, N. Shibata, S. Suzuki and Y. Okawa, “Identification of the Antigenic Determinants of Factors 8, 9, and 34 of Genus Candida,” FEBS Letters, Vol. 395, No. 2-3, 1996, pp. 109-112. doi:10.1016/0014-5793(96)01013-7
[13] W. J. Crozier, “Two Cases of Onychomycosis Due to Cadida zeylanoides,” Australasian Journal of Dermatology, Vol. 34, No. 1, 1993, pp. 23-25. doi:10.1111/j.1440-0960.1993.tb00842.x
[14] K. N. Prasad, J. Agarwal, A. K. Dixit, D. P. Tiwari, T. N. Dhole and A. Ayyagari, “Role of Yeasts as Nosocomial Pathogens & Their Susceptibility to Fluconazole & Amphotericin B,” Indian Journal of Medical Research, Vol. 110, 1999, pp. 11-17.
[15] Y. Miyakawa, K. Kagaya and Y. Fukazawa, “Production and Characterization of Agglutinating Monoclonal Antibodies against Predominant Antigenic Factors for Candida albicans,” Journal of Clinical Microbiology, Vol. 23, No. 5, 1986, pp. 881-886.
[16] H. Kobayashi, N. Shibata, H. Mitobe, Y. Ohkubo and S. Suzuki, “Structural Study of Phosphomannan of Yeast-Form Cells of Candida albicans J-1012 Strain with Special Reference to Application of Mild Acetolysis,” Archives of Biochemistry and Biophysics, Vol. 272, No. 2, 1989, pp. 364-375. doi:10.1016/0003-9861(89)90230-0
[17] H. Kobayashi, J. Suzuki, S. Tanaka, Y. Kiuchi, H. Oyamada, N. Iwadate, H. Suzuki, N. Shibata, S. Suzuki and Y. Okawa, “Structure of a Cell Wall Mannan from the Pathogenic Yeast, Candida catenulata: Assignment of 1H Nuclear Magnetic Resonance Chemical Shifts of the Inner α-1,6-Linked Mannose Residues Substituted by a Side Chain,” Archives of Biochemistry and Biophysics, Vol. 341, No. 1, 1997, pp. 70-74. doi:10.1006/abbi.1997.9939
[18] M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith, “Colorimetric Method for Determination of Sugars and Related Substances,” Analytical Chemistry, Vol. 28, No. 3, 1956, pp. 350-356. doi:10.1021/ac60111a017
[19] O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall, “Protein Measurement with the Folin Phenol Reagent,” Journal of Biological Chemistry, Vol. 193, No. 1, 1951, pp. 265-275.
[20] B. N. Ames and D. T. Dubin, “The Role of Polyamines in the Neutralization of Bacteriophage Deoxyribonucleic Acid,” Journal of Biological Chemistry, Vol. 235, No. 3, 1960, pp. 769-775.
[21] H. Kobayashi, N. Shibata, M. Watanabe, M. Komido, N. Hashimoto, K. Hisamichi and S. Suzuki, “Mild Acetolysis and NMR Studies of the D-Mannan of Saccharomyces cerevisiae X2180-1A Wild-Type Strain,” Carbohydrate Research, Vol. 231, 1992, pp. 317-323. doi:10.1016/0008-6215(92)84028-Q
[22] H. Kobayashi, H. Oyamada, N. Iwadate, H. Szuki, H. Mitobe, K. Takahashi, N. Shibata, S. Suzuki and Y. Okawa, “Structural and Immunochemical Characterization of β-1,2-Linked Mannobiosyl Phosphate Residue in the Cell Wall Mannan of Candida glabrata,” Archives of Microbiology, Vol. 169, No. 3, 1998, pp. 188-194. doi:10.1007/s002030050559
[23] N. Shibata, A. Suzuki, Y. H. Kobayashi and Y. Okawa, “Chemical Structure of the Cell-Wall Mannan of Candida albicans Serotype A and Its Difference in Yeast and Hyphal Forms,” Biochemical Journal, Vol. 404, No. 3, 2007, pp. 365-372. doi:10.1042/BJ20070081
[24] H. Oyamada, Y. Ogawa, N. Shibata, Y. Okawa, S. Suzuki and H. Kobayashi, “Structural Analysis of Cell Wall Mannan of Candida sojae, a New Yeast Species Isolated from Defatted Soybean Flakes,” Archives of Microbiology, Vol. 189, No. 5, 2008, pp. 483-890. doi:10.1007/s00203-007-0339-1
[25] N. Shibata and Y. Okawa, “Enzymatic Synthesis of New Oligosaccharides using Mannosyltransferases from Candida Species and their NMR Assignment,” Biological & Pharmaceutical Bulletin, Vol. 33, No. 5, 2010, pp. 895-899. doi:10.1248/bpb.33.895

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.